GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Application
IF/ICC, WB
Host species
Kaninchen
Reactive species
Affe, Allerdings, Huhn, Kuh, Maus, Mensch, Rat, Schwein
Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.
Wirtsspezies
Kaninchen
Spezifität
NIP7
Immunogen
A synthetic peptide derived from human NIP7
Positive Kontrolle
WB: A549 and HEK293 cell lysates. IF/ICC: A549 cells.
Konjugation
Unconjugated
Note
Carrier Free, ExactAb™, Validated
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Immunogen affinity purified
Konzentration
1.0 mg/mL
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
NIP7 Antibody (Ab118094) - Western Blot All lanes: NIP7 Antibody (Ab118094) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 100.0 s
NIP7 Antibody (Ab118094) - IF/ICC Immunofluorescent analysis of NIP7 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with NIP7 Antibody (Ab118094) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Anwendung
Anwendung
Dilution info
WB
1/500-1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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