NVX-207 - 10mM in DMSO , CAS No.745020-66-0

CAS: 745020-66-0 Cat. No.: N655855 Summenformel: C₃₆H₅₉NO₆ Molekulargewicht: 601.86 PubChem CID: 9960374

QUALITÄT & REINHEIT 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Übersicht

NVX-207, a Betulinic acid-derived anti-cancer compound, shows anti-tumor activity (mean IC 50 =3.5 μM) against various human and canine cell lines. NVX-207-induced apoptosis is associated with activation of the intrinsic apoptotic pathway via cleavage of caspases -9, -3, -7 and of PARP.

In Vitro

NVX-207 induces cell death via apoptosis. NVX-207 has a high cytotoxicity with IC 50 values ranging from 7.6-8.5 µM, in the three analyzed malignant glioma cell lines. NVX-207 leads to PARP cleavage and to a decrease in Survivin expression levels under normoxic and hypoxic conditions. NVX-207 (20 µM) causes a significantly high rate of necrosis of glioma cell lines. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Intravenous application of NVX-207 in mice is well tolerated . MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay

Cytotoxic activities are evaluated using the Sulforhodamine-B (SRB) assay. Exponentially growing cells are seeded into 96-well plates at cell densities to prevent confluence for 96 h. After 24 h, the cells are treated using a dilution series of the compounds for 72 h under normoxic or hypoxic conditions. After treatment, the adherent cells are fixed using 10% TCA at 4°C for 1 h; the cells are washed with ice-cold water and are dyed using 100 µL of 4.4% SRB solution for 10 min. After staining, the plates are washed with 1% acetic acid and air-dried overnight. Three hundred microliters of 20 mM Tris base solution is added, and the absorbance is measured at 540 nm using a 96-well plate reader. The IC 50 values indicate the concentrations of the compound that cause 50% cell inhibition. The data are obtained in three independent experiments. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Spezifikationen

Spezifikationen & Reinheit

10mM in DMSO

Biochemische und physiologische Mechanismen

NVX-207, a Betulinic acid-derived anti-cancer compound, shows anti-tumor activity (mean IC 50 =3.5 μM) against various human and canine cell lines. NVX-207-induced apoptosis is associated with activation of the intrinsic apoptotic pathway via cleavage of

Storage

Store at -80°C

Verschickt in

Dry ice packs + Cold packs

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Namen und Kennungen

Isomere SMILES

CC(=C)[C@@H]1CC[C@]2([C@H]1[C@H]3CC[C@@H]4[C@]5(CC[C@@H](C([C@@H]5CC[C@]4([C@@]3(CC2)C)C)(C)C)OC(=O)C)C)C(=O)OCC(CO)(CO)N

Alternative CAS-Nummer

745020-66-0

PubChem CID

9960374

MeSH-Eintrag

2-amino-3-hydroxy-2-hydroxymethylpropyl 3-acetylbetulinoate;NVX-207

Molekulargewicht

601.86

Application Protocols

• Item-specific (from Product Data)

  • Tested applications, assay formats, or recommended concentrations: Not specified for this item.

• General guidance for small-molecule screening (informational only)

  • Prepare a fresh DMSO stock (e.g., 10 mM), sterile-filter if compatible, and store aliquots at −80 °C. Thaw on ice, minimize exposure to ambient humidity, and avoid repeated freeze–thaw.
  • Perform an initial cytotoxicity or viability titration in the relevant cell system to establish non-toxic solvent/compound ranges.
  • For enzyme assays, include appropriate blanks (substrate-only, inhibitor-only) and verify linearity of the signal in the presence of vehicle.
  • Document exact lot number, preparation date, and storage conditions in ELN/LIMS for reproducibility.

Refer to the SDS and CoA for any item-specific instructions when available.

Biological Roles

Item-specific biological function or target annotation for NVX-207 is not provided in this listing.

• Item-specific (from Product Data)

  • No biological target, pathway, or MoA data specified; Research Use Only.

• Literature guidance (to be verified by the user)

  • Use the CAS (745020-66-0) or PubChem CID (9960374) to retrieve peer-reviewed reports, if any, describing cellular targets, binding affinities, or phenotypic effects. Confirm the exact chemical form (free base/salt, polymorph) matches the literature when comparing activity.

• Experimental best practices (general)

  • Establish compound purity and identity before biological testing (HPLC, LC–MS, 1H NMR where feasible).
  • Determine solubility and stability in relevant media (culture medium with/without serum, assay buffers) and perform time-course integrity checks.
  • Evaluate nonspecific effects: aggregation, redox cycling, membrane disruption, or fluorescence interference (if optical readouts are used).
  • Include appropriate controls: vehicle, positive controls (known actives for the pathway), and orthogonal assays to discriminate on-target from off-target effects.

No clinical or therapeutic claims are made. This material is furnished strictly for laboratory research.

Buffer Applications

NVX-207 is a small organic compound and is not itself a buffering agent.

• Item-specific (from Product Data)

  • No buffer roles or recipes specified.

• Practical notes (general)

  • If NVX-207 must be formulated in aqueous buffer for biological assays, select the buffer based on the target system (e.g., PBS, HEPES, Tris) and verify compound solubility and stability across the working pH. Titrate pH only after the compound is fully dissolved (often via a co-solvent such as DMSO) to avoid precipitation.
  • Monitor adsorption to plastics and precipitation upon dilution. Keep final DMSO ≤0.1–0.5% v/v in cell-based assays when possible to minimize solvent effects.

For guidance on solvent and formulation, see the Solvent Selection and Storage & Reconstitution sections.

Green Alternatives

Because the structure and solvent preferences for NVX-207 are not specified, “greener” considerations focus on dissolution media and handling practices rather than replacing the active compound itself.

• Solvent choices (general guidance)

  • Prefer bioassay stocks in water or buffer when chemically feasible (adjust pH/ionic strength), minimizing DMSO content in final assay wells to ≤0.1–0.5% v/v.
  • When organic solvents are required, consider greener options compared with traditional choices:

  | Use case | Conventional | Greener alternative (general) | Notes | |---|---|---|---| | Stock solutions | DMSO, DMF, NMP | DMSO (retain), 2-MeTHF (for extractions), Cyrene in limited contexts | DMSO remains common for stocks; DMF/NMP have higher regulatory burdens. | | Workup/extraction | DCM, toluene, EtOAc | EtOAc, 2-MeTHF, MTBE | Reduce halogenated solvent use where feasible. | | Crystallization | ACN, toluene | EtOAc/hexanes, IPA/water | Tailor to compound polarity and stability. |

• Operational steps

  • Scale solutions to immediate need to cut solvent waste; use micro-volumes and sealed 96-well plates for screening.
  • Employ solvent recovery where practical and segregate halogenated from non-halogenated waste.

Note: Solvent substitutions must be validated for compound stability and assay performance.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or manufacturing application is provided for this item.

• Item-specific (from Product Data)

  • Pharmaceutical use: Not specified; Research Use Only.

• General context (non-clinical)

  • Compounds in screening libraries like NVX-207 are typically utilized in discovery research (target validation, SAR), not as formulated drug substances or excipients. Any extrapolation to formulation development requires comprehensive preformulation studies (solubility, pKa, polymorphism, stability) and is outside the scope of this catalog listing.

• Compliance note

  • This product is not intended for human or veterinary use, clinical diagnostics, or as an active pharmaceutical ingredient. No therapeutic claims are made.

Physical Properties

Item-specific values are not available in the provided product data.

• Item-specific (from Product Data)

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

• Literature/computed properties (not item specifications)

  • Boiling point, melting point, density, refractive index, logP/logD, pKa, UV-Vis λmax, and solubility parameters: Not provided here. Retrieve from literature or compute from the registered structure (CAS 745020-66-0; CID 9960374). Treat all such values as approximate until verified for your supplied form.

• Practical guidance (general chemistry knowledge)

  • If the solid/liquid state is unknown, perform a small-scale thermal profile: DSC/MP determination or simple capillary melt test to inform handling and storage.
  • Establish a solubility panel empirically at small scale (1–5 mg): DMSO, DMF, acetonitrile, ethanol, isopropanol, methanol, ethyl acetate, acetone, toluene, water, and PBS (if aqueous use is intended). Record clarity time, precipitation upon dilution, and stability at room temperature and 37 °C.
  • For spectroscopic work, acquire UV-Vis and FTIR quickly to flag chromophores or labile groups. If instability is suspected, run an LC–MS time-course (0, 2, 24 h) at intended assay conditions to quantify degradation.

Note: Do not treat any literature property as a product specification; verify against the lot CoA.

Quality and Grades

• Item-specific (from Product Data)

  • Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

• How to interpret grades for screening compounds (general guidance)

  • Bioactive screening compounds are commonly offered at ≥95% purity by HPLC with supporting chromatograms and LC–MS. However, this is not guaranteed for this item unless stated on its CoA.
  • If the compound can exist as multiple stereoisomers/tautomers, confirm isomeric purity and form. Residual solvent levels, water content (Karl Fischer), and inorganic residue may be reported on the CoA.
  • For analytical work (e.g., cellular assays, target engagement), low non-volatile residue and well-defined identity (HRMS/NMR) are desirable. For synthetic use, impurity profiles (e.g., residual halides, metals) may be more critical.

• Recommendations

  • Request and review the CoA/Spec Sheet for your lot for: assay (HPLC/UPLC), identity (1H NMR/HRMS), and any stabilizers/additives.
  • If UV transparency or LC baseline is important, verify UV cutoff/absorbance—Not specified for this item; refer to CoA/Spec Sheet.
  • For regulated workflows, document vendor qualifications and traceability (lot number, expiry/ret-test date, storage history).

Reaction and Applications

This product is positioned as a small-molecule screening compound rather than a synthetic reagent; no manufacturer application notes are supplied.

• Item-specific (from Product Data)

  • Manufacturer Applications: Not specified.

• General research uses (general knowledge)

  • Such compounds are typically utilized in: target validation screens, phenotypic assays, mechanism-of-action studies, and SAR benchmarking. Without target annotation for NVX-207 in this listing, users should consult literature via CAS 745020-66-0 or CID 9960374 to identify any reported bioactivity and then design assays accordingly.

• If employing in chemical biology

  • Establish dose–response curves (8–12 points) with appropriate vehicle controls.
  • Confirm compound integrity under assay conditions by LC–MS, and monitor for aggregation artifacts (e.g., using detergent controls or DLS).
  • If covalent reactivity is suspected from literature, include nucleophile scavengers or time-dependence controls.

• Not typically applicable here

  • Named synthetic reactions (e.g., cross-couplings, condensations) are not described for this catalog item. If you intend to use NVX-207 as a building block, first verify functional handles present in the structure (refer to literature) and consider protecting-group and compatibility constraints.

Reaction Conditions

No reaction conditions are specified for NVX-207, and this product is not supplied as a synthetic reagent in this listing.

• Item-specific (from Product Data)

  • Reaction guidance: Not specified.

• General considerations (only if structure is retrieved externally)

  • Choose solvents and temperatures based on the compound’s functional groups and stability. For example, amide couplings typically proceed in DMF/DCM with carbodiimides or uronium reagents; cross-couplings often use Pd catalysts in polar aprotic media. These are general examples and are not specific to NVX-207.
  • Conduct small-scale trials and monitor by LC–MS/NMR. Protect from moisture/oxygen/light if sensitive motifs are present (confirm from structure/SDS).

In the context of this catalog entry, prioritize the Solvent Selection and Storage & Reconstitution sections for practical handling rather than reaction setup.

Safety and Handling

Safety information provided for this listing is minimal; handle as a potentially hazardous small molecule.

• Item-specific (from Product Data)

  • GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
  • Storage: Store at −80 °C.
  • Shipping: Dry ice packs + cold packs.

• General laboratory precautions (defer to SDS as authoritative)

  • PPE: Lab coat, safety glasses, and suitable chemical-resistant gloves (e.g., nitrile). Use in a chemical fume hood to avoid inhalation of dusts/vapors/aerosols.
  • Avoid contact with skin, eyes, and clothing. Prevent breathing dust/fume. Wash thoroughly after handling.
  • Incompatibilities: Unknown for this item. Until defined by SDS, avoid strong oxidizers/reducers, strong acids/bases, and reactive metals. Keep away from ignition sources if solvent is flammable during preparation.
  • First aid (general): If inhaled—move to fresh air; if on skin—wash with soap and water; if in eyes—rinse cautiously with water for several minutes; if ingested—rinse mouth, do not induce vomiting; seek medical attention. Bring SDS.

• Risk management

  • Assume potential light/moisture/oxygen sensitivity; minimize exposure during weighing and solution prep. Use amber vials where appropriate.
  • For bioassays, prepare fresh aliquots and limit freeze–thaw cycles (see Storage & Reconstitution). Dispose of waste according to institutional and local regulations.

Always consult the product-specific SDS and CoA for definitive hazard and handling guidance.

Solvent Selection

With structure not provided in the listing, choose solvents empirically, starting from high-solvating polar aprotics and moving outward.

• Item-specific (from Product Data)

  • No solubility specifications are provided; refer to CoA/Spec Sheet.

• General recommendations (general chemistry knowledge)

  • Primary stock solutions for bioassays: DMSO (anhydrous) is the first-line solvent for many small molecules; typical stock 10–50 mM. Confirm stability at room temperature and after freeze–thaw.
  • Alternative strong solvents: DMF, NMP, or acetone if DMSO insoluble or unstable.
  • Semi-polar organics: ACN, EtOH, MeOH, i-PrOH, EtOAc can be leveraged depending on functionality.
  • Aqueous work: Assess direct water or buffer solubility after pH adjustment (pH 2–10), using co-solvent strategies (≤1–2% DMSO or 5–20% ethanol) and surfactants only if compatible with the assay.

• Miscibility and polarity considerations (general)

  • Map solubility versus dielectric constant and hydrogen-bonding parameters. Start with micro-scale titrations (1–5 mg) and quantify by UV/LC.

• Practical notes

  • Filter sterilize (0.22 µm PTFE) only if known to be stable and non-adsorptive to filters.
  • Minimize adsorption losses by pre-rinsing plastics or using glass vials for hydrophobic compounds. Record precipitation upon dilution into aqueous media.

Storage and Reconstitution

• Item-specific (from Product Data)

  • Storage conditions: Store at −80 °C.
  • Shipping: Dry ice packs + cold packs to maintain low temperature during transit.

• General best practices for small-molecule stocks (general knowledge)

  • Upon receipt: Allow container to equilibrate to room temperature in a desiccator before opening to prevent condensation. Inspect for visible changes (clumping, discoloration) and document lot info.
  • Aliquoting: If solid, subdivide into single-use portions under dry, inert conditions if feasible. If preparing a solution stock (commonly in anhydrous DMSO), filter if compatible and dispense into amber, airtight vials or 96-well plates with heat seals.
  • Concentrations: Typical master stocks 10–50 mM in DMSO. Confirm solubility empirically; do not force dissolution with heat if stability is unknown.
  • Freeze–thaw: Avoid repeated cycles. Store aliquots at −80 °C; for routine screening, working aliquots may be kept at −20 °C short-term if stability allows (verify by LC–MS/HPLC over time).
  • Light and moisture: Protect from light (foil/amber) and humidity (desiccant). Purge headspace with inert gas for oxygen-sensitive materials.
  • Stability/expiry: Not specified for this item; refer to CoA/Spec Sheet. Establish in-house retest dates based on periodic QC (purity by HPLC, identity by MS).

These practices help preserve integrity for reproducible research use only.

Structure and Identity

Concise identity summary for a small-molecule research compound distributed for screening applications.

• Item-specific (from Product Data)

  • Product name: NVX-207 (SKU: N655855)
  • CAS: 745020-66-0
  • PubChem CID: 9960374
  • InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

• Literature/registry pointers (general)

  • With the CAS and CID above, structural details (systematic name, stereochemistry, tautomeric form) can be retrieved from standard databases (PubChem, Reaxys, CAS Registry). Always verify the exact tautomer/salt/solvate form that matches your lot/CoA.

• Structural description (general guidance)

  • Without the SMILES/InChI for this catalog entry, a precise 2D description cannot be provided here. Consult the SDS/CoA or the cited registries for: functional groups present, ring systems, heteroatoms, formal charge, and stereocenters.
  • If multiple polymorphs, salts, or solvates exist in the literature for NVX-207, confirm which form is supplied by checking the CoA and HRMS/NMR traces (if provided). These details can impact solubility, melting behavior, and assay response.

• Best practice

  • Cross-confirm the lot’s exact identity by matching CAS + InChIKey from the CoA/SDS to your ELN/LIMS entries prior to screening or synthesis.

Synthetic Utility

This listing does not position NVX-207 as a building block or reagent, and no functional-group information is disclosed here.

• Item-specific (from Product Data)

  • Synthetic role: Not specified.

• General guidance if considering synthetic use (general chemistry knowledge)

  • Retrieve the structure (via CAS/CID) and identify reactive handles (halides, aldehydes/ketones, carboxylates, amines, heteroaromatics). Confirm protecting-group compatibility and potential chemoselectivity issues.
  • If multiple heteroatoms or sensitive motifs are present (e.g., nitro, diazo, Michael acceptors), assess stability to bases/acids, nucleophiles, and reductants before planning transformations.
  • Validate each step by LC–MS and, when applicable, chiral HPLC if stereochemical integrity is critical.

• Caution

  • Without verified structure and purity data for this shipped material, repurposing it for synthesis carries risk; obtain a reagent-grade version or confirm specifications via CoA first.

Target Specificity

• Item-specific (from Product Data)
  • Target, pathway, enzyme/receptor selectivity, and binding constants: Not specified for this item; refer to literature using CAS 745020-66-0 or PubChem CID 9960374, and verify against the supplied lot.

No claims of biological target specificity are made in this listing.

Häufig gestellte Fragen

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 745020-66-0, the molecular formula is C36H59NO6, and the molecular weight is 601.86 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.