Pectate Lyase, CAS No.9015-75-2

CAS: 9015-75-2 Cat. No.: P1420316 EC Number: 232-766-9

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Aspergillus sp.; ~1400 U/mL; ≥180 U/mg protein

Accession #

Q9C2Z0

Bioactivity

≥180 U/mg protein

Overview

High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and analytical testing applications.

Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.

PROPERTIES

1. ELECTROPHORETIC PURITY:

- Single major band on SDS-gel electrophoresis (MW = ~ 45,000)

2. SPECIFICITY:

Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.

3. PHYSICOCHEMICAL PROPERTIES:

pH Optima: 8.0

pH Stability: 6.5-11.0

Temperature Optima: 55°C

Temperature Stability: < 55°C

4. STORAGE CONDITIONS:

The enzyme is supplied as a solution containing 50% glycerol and 0.02% (w/v) sodium azide and should

be stored below -10°C. For assay, this enzyme should be diluted in Tris.HCl buffer (100 mM), pH 8.0.

Swirl to mix the enzyme immediately prior to use.

The enzyme is used for the identification of Pectin in foodstuffs, feed and fruit juices.

Specifications

Product Name

Pectate Lyase, CAS No.9015-75-2

Synonyms

(1→4)-alpha-D-galacturonan lyase | alpha-1, 4-D-endopolygalacturonic acid lyase | endo-alpha-1, 4-polygalacturonic acid lyase | endogalacturonate transeliminase | endopectin methyltranseliminase | pectate transeliminase | pectic acid lyase | pectic acid tra

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Aspergillus sp.; ~1400 U/mL; ≥180 U/mg protein

Biochemical and Physiological Mechanisms

Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal

Bioactivity

≥180 U/mg protein

Accession #

Q9C2Z0

CAS

9015-75-2

Enzyme Commission Number

EC 4.2.2.2

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Aspergillus sp.; ~1400 U/mL; ≥180 U/mg protein

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One Unit of pectate lyase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid per minute from polygalacturonic acid (2.5 mg/mL) in Tris.HCl buffer (50 mM), pH 8.0 at 40°C.