Determine the necessary mass, volume, or concentration for preparing a solution.
BioReagent, sterile-filtered BioReagent,Steril gefiltert für empfindliche chromatographische und analytische Workflows, die minimale Basislinieninterferenzen erfordern.
Store at 2-8°C,Protected from light,Do not freeze Versand Wet ice,Do not freeze. Genaue Spezifikationen finden Sie im chargenspezifischen COA.
SDS, COA, Datenblatt und Spezifikationsblatt stehen zum Download bereit. Chargenspezifische COA über Chargennummernsuche abrufbar.
In 0 peer-reviewten Publikationen zu Chromatographie, organischer Synthese und Kreuzkupplungsreaktionen zitiert.
The fixative stabilizes the cell membrane and preserves the binding of antibodies to antigens on the cell surface. The Permeabilization Buffer creates pores in the fluid, intact cell membrane to allow antibodies to enter the cell. For the detection of intracellular antigens and DNA by flow cytometry, cells are fixed and permeabilized, then incubated with the corresponding antibodies or detection reagents before analysis.
Instructions for Use:
Harvest the cells to be tested and centrifuge at 1000 rpm for 10 minutes. Discard the supernatant.
Add 500 µL of pre-chilled (4°C) fixative.
Incubate at room temperature for 10 minutes.
Centrifuge at 1000 rpm for 10 minutes.
Discard the supernatant. Wash the cells once with PBS, then centrifuge at 1000 rpm for 10 minutes.
Discard the supernatant. Add 1.5 mL of permeabilization agent.
Incubate at room temperature for 10-15 minutes.
Centrifuge at 1000 rpm for 5 minutes.
Discard the supernatant. Resuspend the cells in an appropriate volume of permeabilization agent.
Add the detection antibody and stain for 15 minutes.
Wash the cells once with PBS and centrifuge at 1000 rpm for 5 minutes.
Discard the supernatant. Resuspend the cells in 200 µL of PBS for analysis on the flow cytometer.
Precautions
The Permeabilization Buffer contains 0.05% ProClin 300. Avoid contact with skin, eyes, and mucous membranes during use.
This product is for research use only. Not for use in clinical diagnosis.
Umfassendes Dokument zu Gefahren, Handhabung, Lagerung und Einhaltung regulatorischer Vorschriften.
<<<<<<< HEAD SDS herunterladen → ======= SDS herunterladen → >>>>>>> release248-p3Chargenspezifische Qualitätsdaten. Geben Sie Ihre Chargennummer ein, um das genaue COA abzurufen.
COA nachschlagen →Vollständige Qualitätsmerkmale und Akzeptanzkriterien für diese Qualität.
Spezifikationsblatt ansehen →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Datum | Artikel |
|---|---|---|---|
| Certificate of Analysis | Jul 29, 2026 | M1373920 |
| Empfindlichkeit | Light-sensitive |
|---|
Unser Leitfaden zur Qualitätsauswahl behandelt Reinheit, Stabilisatorstatus und Anwendungseignung für alle Varianten in unserem Katalog.
Sterile-filtered Qualitätsleitfaden ansehen → BioReagent Qualitätsleitfaden ansehen →