Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

CAS: 9029-91-8 Cat. No.: P1431341 EG-Nummer: 232-855-2

QUALITÄT & REINHEIT Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥3000 U/mL; ~3500 U/mg protein

Accession #

P39646

Bioactivity

≥3000 U/mL; ~3500 U/mg protein

Übersicht

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications. 

Specificity: 

Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Temperature Optima: 25°C

pH Optima: 7.4

Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

PROPERTIES 

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW ~ 36,953) 

- Single major band on isoelectric focusing (pI ~ 5.3) 

2. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 7.4 and up to 25°C. 

3. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension containing 0.5 mg/mL BSA and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use. 

Spezifikationen

Product Name

Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

Synonyme

Acetyl-CoA:orthophosphate acetyltransferase | phosphate acetyltransferase | phosphoacylase | phosphotransacetylase | PTA

Note

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Spezifikationen & Reinheit

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥3000 U/mL; ~3500 U/mg protein

Bioaktivität

≥3000 U/mL; ~3500 U/mg protein

Beitritt #

P39646

Voraussichtliches Molekulargewicht

36,953

CAS

9029-91-8

Enzym Kommissionsnummer

EC 2.3.1.8

Molekültyp

Enzyme

Product Line

Biologische Produkte

Lagerung und Versand

Konzentration

≥3000 U/mL; ~3500 U/mg protein

Storage

Store at 2-8°C

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Definition der Einheit

One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25°C.