PI5P4Kγ-IN-1 - Moligand™, 10 mM in DMSO , CAS No.2419358-87-3

CAS: 2419358-87-3 Cat. No.: P1497979 Summenformel: C₂₀H₂₀N₄O Molekulargewicht: 332.40

GRADE & PURITY Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Übersicht

PI5P4Kγ-IN-1 (compound 2) is a selective PI5P4Kγ inhibitor. PI5P4Kγ-IN-1 can be used to signal mTORC1 in MCF-7 breast cancer cells and further characterize PI5P4Kγ in the cells.

Specifications

Spezifikationen & Reinheit

Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Verschickt in

Dry ice packs + Cold packs

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

Moligand™

Namen und Kennungen

Molekulargewicht

332.40

Application Protocols

Only provide protocols from product data. For this item, no tested applications or protocols are specified.

• Recommended assay types: Not specified for this item; refer to CoA/Spec Sheet.
• Suggested working concentrations or dilutions: Not specified for this item; refer to CoA/Spec Sheet.
• Positive controls/standards: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (non-binding, generic for small-molecule probes):

• Prepare single-use DMSO aliquots at defined concentration (e.g., 10 mM). Thaw immediately before use, dilute into assay buffer, and discard remainder.
• Include vehicle controls matching final DMSO content. Consider dose–response with adequate replicates and incorporate orthogonal assays to confirm on-target effects.

Biological Roles

General pathway context (literature/generic; not item-specific claims):

• PI5P4Kγ (phosphatidylinositol-5-phosphate 4-kinase gamma; also PIP4K2C) is a lipid kinase that phosphorylates PI(5)P to produce PI(4,5)P2, contributing to the cellular phosphoinositide pool. It participates in membrane dynamics, signaling, and metabolic regulation.
• The PI5P4K family (α/β/γ) influences processes such as endomembrane trafficking, stress responses, and nuclear signaling, with isoform-specific cellular localizations and regulatory roles.
• Small-molecule inhibitors of PI5P4Kγ are used as chemical probes to interrogate isoform-specific function, dissect compensatory mechanisms among PI5P4K isoforms, and study lipid signaling crosstalk.

Research implications:

• Chemical inhibition can complement genetic perturbation to evaluate acute vs adaptive effects on phosphoinositide metabolism.
• Downstream readouts may include changes in PI(5)P/PI(4,5)P2 levels, actin dynamics, vesicular trafficking markers, and transcriptional responses.

Note: No biological potency, selectivity profile, or cellular phenotypes are specified for this item. Users should consult primary literature and generate internal controls (e.g., inactive analogs, rescue experiments) to attribute observed effects to PI5P4Kγ modulation.

Buffer Applications

Not typically applicable. PI5P4Kγ-IN-1 is a small-molecule research probe rather than a buffering agent. For assay work, standard biological buffers (e.g., HEPES, PBS, Tris) are chosen based on the biology under study.

General guidance (generic):

• Prepare DMSO stocks and dilute into the chosen assay buffer, keeping the final DMSO percentage within validated limits for your system.
• Consider adding carrier proteins (e.g., 0.1% BSA) or nonionic surfactants (e.g., 0.01% Tween-20) only if needed to prevent nonspecific adsorption; validate for your assay. Item-specific compatibility is not provided—determine empirically.

Green Alternatives

Because PI5P4Kγ-IN-1 is a research tool compound rather than a process solvent or bulk reagent, the concept of greener alternatives focuses on solvent handling and assay formats rather than substitution of the active itself.

Greener handling considerations (general):

• Prefer aqueous-compatible workflows that minimize DMSO content while maintaining solubility and assay performance.
• Use miniaturized assay volumes (e.g., 96/384/1536-well formats) to reduce chemical use and waste generation.
• Implement solvent recycling for wash steps and analytical runs where feasible.

Solvent comparison for stock solutions (generic; not item-specific):

• DMSO: High solvency, widely accepted in bioassays; moderate environmental impact; ensure capped, low-evaporation handling.
• Ethanol: Potentially greener but often less effective for hydrophobic inhibitors and may perturb biological assays.

Waste minimization:

• Plan aliquots to avoid freeze–thaw cycles and discard; right-size aliquots for single-use to reduce waste.

Note: No greener “alternative inhibitor” can be recommended without item-specific structure and performance criteria. Select the probe based on scientific fit; implement green lab practices around its use.

Pharmaceutical Uses

Not applicable for this catalog item. PI5P4Kγ-IN-1 is supplied strictly for research use only and is not intended for human or veterinary use, drug substance manufacture, or as an excipient.

General remarks (non-clinical, non-therapeutic):

• In preclinical research, such tool compounds can support target validation and mechanism studies that inform discovery decisions. Any translation to formulation development would require comprehensive CMC characterization and regulatory-grade materials, which are beyond the scope of this research reagent.

Item-specific pharmacopeial status, residual solvents, elemental impurities, and stability-indicating methods: Not specified for this item; refer to CoA/Spec Sheet if available.

Physical Properties

Item-specific data:

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not applicable/unknown for a non-volatile solid; specific value not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, logP, pKa, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/generic for screening compounds; not item-specific specifications):

• Many kinase-focused small molecules are supplied as solids intended for dissolution in anhydrous DMSO at 10–50 mM to create screening stocks; aqueous solubility varies widely and often requires cosolvent (DMSO) or surfactant.
• Hygroscopicity, polymorphism, and ionization state can affect handling and solubility; verify using the item’s CoA before preparing solutions.

Quality and Grades

Item-specific grade:

• Grade/Purity: Moligand™ (as listed). Specific purity metrics and stabilizers: Not specified for this item; refer to CoA/Spec Sheet.

What Moligand™ typically denotes (general, catalog-wide context):

• Moligand™ items are curated, research-grade small molecules intended for target engagement, screening, and chemical biology. They are typically quality-controlled for identity (e.g., NMR/HRMS/LC-MS) and purity suitable for discovery workflows. Exact QC panels and thresholds are item-specific and provided on the CoA.

Implications for use:

• Chromatographic behavior, salt form, residual solvent levels, and water content can influence assay performance. Since these are not specified here, confirm with the CoA if your application requires defined limits (e.g., quantitative biophysics, crystallography).
• Low-UV impurity control may be important for plate-based readouts; if relevant, verify HPLC traces on the CoA.

Traceability:

• Reference SKU P1497979 and CAS 2419358-87-3 in documentation. Retain the batch CoA for auditability and method validation.

Reaction and Applications

This product is a bioactive small molecule intended for research use as a chemical probe/inhibitor. It is not commonly used as a synthetic reagent or catalyst.

General applications in research (generic to PI5P4K pathway chemical biology; not item-specific claims):

• Tool compound use in target validation: Modulation of PI5P4Kγ activity in cell-based assays to probe lipid signaling pathways and downstream phenotypes.
• Pathway dissection: Combination with genetic approaches (e.g., siRNA/CRISPR) to confirm on-target effects via orthogonal validation.
• Chemical proteomics (when applicable): Affinity or photoaffinity derivatives of inhibitors can be used to assess target engagement; applicability depends on structure—consult the CoA and literature.

Assay formats (generic):

• Biochemical kinase/lipid kinase activity assays, lipid substrate turnover measurements, and cellular readouts sensitive to phosphoinositide levels.

Note: No specific potency, selectivity, or application data are provided for this item. Refer to the CoA/Spec Sheet and primary literature for detailed protocols and performance characteristics.

Reaction Conditions

Not applicable. PI5P4Kγ-IN-1 is not supplied for use as a synthetic reagent or catalyst; therefore, standard synthetic reaction conditions are not relevant.

Assay handling conditions (generic, non-binding guidance for small-molecule probes):

• Stock preparation: Often dissolved in anhydrous DMSO at 10–50 mM, filtered if needed. This is general guidance only; item-specific solubility is not provided.
• Working concentrations: Determined empirically based on biochemical or cellular assay sensitivity; begin with a wide range (e.g., nanomolar to low micromolar) while maintaining acceptable DMSO levels.
• Incubation: Equilibration times vary by target and assay format; pilot time-course experiments (e.g., 15 min to several hours) are recommended to identify steady-state effects.

Note: No item-specific activity, kinetics, or stability data are provided. Consult primary literature and generate internal controls.

Safety and Handling

Item-specific hazard data:

• GHS Classification: Not specified for this item; refer to SDS.
• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (not item-specific; follow institutional SOPs):

• Handle in a chemical fume hood wearing appropriate PPE: lab coat, nitrile gloves, and safety glasses. Avoid inhalation of dust or contact with skin/eyes.
• Avoid generating dust/aerosols. Use micro-spatulas and antistatic measures for milligram-scale weighing.
• Incompatibilities: Avoid strong oxidizers and strong acids/bases until compatibility is known. Keep away from sources of ignition for organic solids.
• First aid (overview; consult SDS for specifics): Inhalation—move to fresh air; Skin—wash with soap and water; Eyes—rinse cautiously with water for several minutes; Ingestion—rinse mouth, seek medical advice.
• Waste: Dispose as organic chemical waste per local regulations; do not discharge to drains.
• Special risks: None specified for this item. As with many bioactive small molecules, minimize exposure and prevent environmental release. Always defer to the product SDS for authoritative guidance.

Solvent Selection

Item-specific solubility data are not provided for this product; consult the CoA/Spec Sheet. The guidance below is general for small-molecule inhibitor stocks and is not a specification.

General solvent strategy (literature/generic):

• Primary solvent: Anhydrous DMSO is commonly used to prepare concentrated stocks (e.g., 10–50 mM), owing to broad solubilizing power for heteroaromatic, moderately lipophilic compounds.
• Secondary solvents/co-solvents: If required, DMF or ethanol can assist initial dissolution before dilution into assay buffer containing a small percentage of DMSO.
• Aqueous buffers: Direct dissolution into water or PBS often gives low solubility; instead, prepare a DMSO stock and dilute into buffer, keeping final DMSO below assay-tolerated thresholds (typically 0.1–1% v/v; determine experimentally).

Comparison (generic):

• DMSO vs DMF: DMSO generally preferred for biocompatibility in screening; DMF is more hygroscopic and less favored for biological assays.
• DMSO vs ethanol: Ethanol can disrupt some protein assays; use sparingly and validate compatibility.

Practical tips:

• Warm gently (up to ambient/room temperature) and vortex/sonicate to aid dissolution; avoid prolonged heating. Filter (0.22 µm) if particulate persists after dilution.

Storage and Reconstitution

Item-specific instructions (from Product Data):

• Storage Conditions: Store at -80°C.
• Shipped In: Dry ice packs + Cold packs.
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Stability notes:

• Long-term storage at -80°C is recommended to minimize degradation and ensure batch consistency. Protect from light and moisture. Avoid repeated freeze–thaw cycles by preparing single-use aliquots.

Reconstitution (general guidance; not item-specific specifications):

• Solvent: Anhydrous DMSO is commonly used for small-molecule inhibitors. If the item-specific solubility or salt form requires alternatives, consult the CoA.
• Procedure: Allow vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation. Weigh quickly, dissolve in dry solvent with vortex/brief sonication, and sterile-filter the working solution if sterility is required for cell-based assays.
• Aliquoting: Dispense into inert, low-bind microtubes (amber if light-sensitive) and refreeze at -80°C. Record concentration and date.

Specifications not provided for this item (refer to CoA/Spec Sheet):

• Exact solubility limits in DMSO/water, pH stability, extinction coefficient, and solution stability over time.

Research use note: For research use only.

Structure and Identity

Overview: PI5P4Kγ-IN-1 is cataloged as a small-molecule inhibitor tool compound targeting phosphatidylinositol-5-phosphate 4-kinase gamma (PI5P4Kγ), intended for research use.

Item-specific identifiers (from Product Data):

• CAS: 2419358-87-3
• SKU: P1497979
• Grade: Moligand™
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural features:

• Item-specific 2D structure and functional groups: Not specified for this item; refer to CoA/Spec Sheet.

General context (literature/generic):

• PI5P4Kγ inhibitors are commonly heteroaromatic, drug-like small molecules designed to engage lipid kinase ATP-binding or allosteric regions. They frequently contain H-bond donors/acceptors and aromatic scaffolds to optimize kinase pocket interactions. This is general information and not specific to this item without the provided structure.

Synthetic Utility

This product is intended as a bioactive probe rather than a synthetic building block, so it is not typically employed as a reagent or intermediate in synthesis.

General considerations (generic):

• If derivatization is desired for chemical biology (e.g., biotinylation, photoaffinity, or fluorophore tagging), feasibility depends entirely on the presence of modifiable positions that preserve target engagement—information not provided here. Users should consult the compound’s structure (CoA) and SAR literature before attempting probe derivatization.
• Stable isotopologues or handle-equipped analogs are often used for chemoproteomics and target engagement assays; these would be separate items with defined structures and are not implied by this listing.

Target Specificity

Only provide information from product data. For this item, specific target-binding data (e.g., Ki, IC50, selectivity vs isoforms/kinases, binding mode) are not provided.

• Claimed target or isoform specificity: Not specified for this item; refer to CoA/Spec Sheet.
• Off-target profile or counterscreens: Not specified for this item; refer to CoA/Spec Sheet.
• Mechanism (ATP-competitive vs allosteric): Not specified for this item; refer to CoA/Spec Sheet.

Note: While the product name suggests PI5P4Kγ engagement, definitive specificity data must be confirmed from the CoA, SDS, or primary literature for the exact compound/batch.

Häufig gestellte Fragen

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2419358-87-3, the molecular formula is C20H20N4O, and the molecular weight is 332.40 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.