pLenti-ARID2-sgRNA

Cat. No.: P743889

Specifications

Product Name

pLenti-ARID2-sgRNA

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at -80°C

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Stored at -20 ℃, valid for 3 months;Stored at -80 ℃, valid for at least one year.

Application Protocols

Item-specific (from Product Data)

• Tested applications, recommended conditions, dilutions, and controls: Not specified for this item; refer to CoA/Spec Sheet.

Important

• Because no application parameters are provided in the Product Data, users should consult the CoA/Spec Sheet for SKU P743889 and adapt general CRISPR/lentiviral best practices to their specific cell model and assay. No protocol is implied by this listing.

Biological Roles

Scope note

• The following summarizes general biology related to ARID2 and CRISPR sgRNA research tools. It is not item-specific and does not imply any medical or clinical use.

General/literature overview (not item-specific)

• ARID2 (AT-rich interactive domain-containing protein 2) encodes a subunit of the SWI/SNF (BAF/PBAF) chromatin-remodeling complexes, which utilize ATP hydrolysis to reposition nucleosomes and regulate access of transcriptional machinery to DNA.
• Functional roles: Regulation of transcriptional programs, coordination of enhancer/promoter accessibility, and participation in DNA damage response pathways have been reported in the literature.
• Research utility of sgRNAs targeting ARID2: Enable interrogation of chromatin remodeling dependencies, transcriptional circuitry, and epigenetic regulation by generating targeted indels via CRISPR-Cas9.
• Readouts: Transcriptomic shifts (RNA-seq), chromatin accessibility (ATAC-seq), histone modification landscapes (ChIP-seq), and functional phenotyping in diverse cell types can elucidate ARID2-linked mechanisms.

Caveats (general)

• Outcomes depend on guide sequence, cell context, and allelic status. Off-target editing and clonal selection biases should be assessed using orthogonal validation strategies.

Note

• Target specificity and guide sequence for this catalog item are not provided; consult the CoA/Spec Sheet to verify target region, PAM context, and any backbone features.

Buffer Applications

Not typically applicable

• This product is a nucleic acid construct used in molecular biology workflows rather than a buffering agent. Use standard nuclease-free aqueous buffers (e.g., Tris-based) appropriate for DNA handling and cell culture.

Practical notes (general; not item-specific)

• DNA handling: Resuspend and store in nuclease-free water or low-salt Tris buffers; avoid divalent cations that can promote nuclease activity unless required by downstream enzymes.
• Cell culture: Ensure any buffer excipients are compatible with the transfection/transduction method and cell viability. Filter-sterilize buffers (0.22 µm) for aseptic work.

Green Alternatives

Applicability note

• As a nucleic acid research reagent, conventional solvent/energy-intensity comparisons used for small-molecule chemistry are not directly applicable.

General/literature considerations for greener molecular biology (not item-specific)

• Delivery modality choices: Where feasible, consider non-viral delivery (electroporation, lipid nanoparticles, RNP delivery of Cas9+sgRNA) to reduce generation of viral biohazard waste and associated decontamination steps.
• Waste minimization: Use miniaturized transfection/transduction volumes; consolidate plasmid prep steps to reduce plastic consumption; select kits with recyclable components.
• Energy and cold-chain: Plan experiments to minimize repeated freezer door openings; aliquot once to reduce freeze–thaw cycles and dry-ice shipments.
• Safer reagents: Prefer enzyme/buffer systems free of hazardous preservatives; avoid phenol/chloroform extractions when column alternatives suffice.

Trade-offs

• Non-viral methods may reduce biosafety burden but can lower delivery efficiency in hard-to-transfect cells; lentiviral methods improve uptake at the cost of higher containment and waste treatment requirements.

Note: No item-specific environmental metrics are provided for SKU P743889.

Pharmaceutical Uses

Not applicable for this catalog item

• pLenti-ARID2-sgRNA is provided for research use only (per Product Data). It is not manufactured or qualified for pharmaceutical formulation, clinical, or diagnostic applications.

General information (not item-specific)

• Similar nucleic acid tools may be used in preclinical research to study gene function or manufacturing workflows for cell engineering research, but translation to GMP-grade materials requires rigorous requalification (identity, purity, endotoxin/bioburden, adventitious agents) and extensive documentation.

Always consult your quality unit and obtain appropriate GMP-grade materials for any clinical or manufacturing use. This catalog item is for research use only.

Physical Properties

Item-specific (from Product Data)

• Physical form/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight, MW distribution: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/solvent: Not specified for this item; refer to CoA/Spec Sheet.

General/literature values and practical notes (not item-specific)

• State: DNA constructs are typically supplied as aqueous solutions or lyophilized solids in nuclease-free buffers (e.g., 10 mM Tris-HCl, 0.1–1 mM EDTA, pH 7.5–8.5) or water.
• Solubility: Highly soluble in nuclease-free water and standard TE-type buffers; insoluble in organic solvents.
• Thermal behavior: No meaningful BP/MP; DNA denatures upon heating and degrades with nucleases and extreme pH.
• UV properties: DNA exhibits a characteristic absorbance maximum at ~260 nm (literature), with A260/A280 ~1.8 indicating relatively pure DNA.
• pKa/logP/refractive index: Not applicable for macromolecular nucleic acids.

Note: Treat all numerical specifications as “Not specified for this item” unless explicitly listed on the CoA/Spec Sheet for SKU P743889.

Quality and Grades

Item-specific (from Product Data)

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

General guidance on nucleic acid reagent quality (not item-specific)

• Sequence identity: For sgRNA vectors, correct guide sequence and scaffold integrity are typically verified by Sanger sequencing using flanking primers. Some suppliers also verify the backbone by restriction mapping or long-read sequencing.
• Endotoxin level: For cell culture applications, low endotoxin DNA (≤10 EU/µg, literature) is often preferred to minimize cytotoxicity during transfection. Value not specified for this item.
• Concentration and purity: Quantified by UV (A260) or fluorometry (dsDNA dye). A260/A280 ~1.8 and A260/A230 >2.0 are typical indicators of acceptable purity (literature). Not specified for this item.
• Contaminants: RNase/DNase-free handling expected; residual solvents/salts should be minimized. Specific limits are not provided for this item.
• Stabilizers: Plasmid DNA is often supplied in Tris/EDTA for stability; the presence/absence of any stabilizer for this item is not specified.

How to use this information

• For regulated work or high-sensitivity applications, request the CoA/Spec Sheet for SKU P743889 to confirm sequence, backbone, antibiotic marker, endotoxin level, and formulation.

Reaction and Applications

Item-specific (from Product Data)

• Manufacturer Applications: Not specified beyond the product name; refer to CoA/Spec Sheet.

General/literature applications of pLenti sgRNA constructs (not item-specific)

• CRISPR-Cas9 genome editing: Delivery of a gene-specific sgRNA (here, name suggests ARID2) to guide Cas9 to a target locus for knockout/knockdown studies.
• Stable cell line generation: Lentiviral delivery enables integration into host genomes for durable sgRNA expression, supporting long-term studies or pooled screens.
• Pooled or arrayed functional genomics: Use in loss-of-function screens with NGS-based deconvolution of guide representation.
• Complementary systems: Compatible with cell lines already expressing Cas9, or co-delivery with a Cas9 expression vector. Inducible Cas9/sgRNA systems can provide temporal control (literature).

Practical considerations (general; not item-specific)

• Guide design: Efficiency and specificity depend on guide sequence, PAM availability, chromatin context, and off-target propensity; validate edits by amplicon sequencing or TIDE/ICE analysis.
• Controls: Include non-targeting and positive-control sgRNAs; incorporate rescue experiments where feasible.
• Delivery: Choose transfection vs. lentiviral transduction based on cell type; primary and non-dividing cells often require lentivirus.
• Selection/enrichment: Many sgRNA vectors include antibiotic resistance or fluorescent reporters to enrich transduced cells; confirm marker and selection conditions from the item’s CoA/Spec Sheet.
• Downstream assays: qPCR for indel quantification, Western blot for protein loss, and phenotypic assays tailored to ARID2 pathways as applicable.

Reaction Conditions

Applicability note

• Classical reaction-condition parameters (solvent, catalyst, yield) do not apply to nucleic acid constructs. Below are general literature guidelines for cell-based use of lentiviral sgRNA vectors; these are not item-specific protocols.

General/literature guidance (not item-specific)

• Transfection (for plasmid delivery): Use nuclease-free buffers and a transfection reagent compatible with your cell line; optimize DNA amount and reagent ratio per manufacturer guidance. Maintain cells at 40–80% confluence.
• Lentiviral packaging: Co-transfect packaging cells (e.g., HEK293T) with transfer vector, packaging plasmids, and envelope plasmid; harvest supernatant 48–72 h post-transfection. Perform all steps under appropriate biosafety level.
• Transduction: Add filtered viral supernatant to target cells with polybrene (if compatible) and determine multiplicity of infection (MOI) empirically. Spinoculation can improve delivery for some cell types (literature).
• Selection and validation: Apply selection (e.g., antibiotic) only if the vector encodes a selectable marker; verify editing by genotyping (NGS or Sanger-based indel analysis) and protein-level assays.
• Controls and replicates: Include non-targeting and positive-control guides; use biological replicates to mitigate clonal variance.

Important

• Specific selectable markers, promoters, and guide sequences for SKU P743889 are not specified; consult the CoA/Spec Sheet for exact conditions.

Safety and Handling

Item-specific (from Product Data)

• GHS Classification / H-Statements / Pictograms / Signal Word: Not specified for this item; refer to SDS.
• Storage conditions: Store at −80°C (provided).
• Shipping: Dry ice packs + cold packs (provided).

General safety guidance (not item-specific; defer to your IBC/SDS)

• Biosafety level: Plasmid DNA itself is typically handled at BSL-1. If used to generate recombinant lentiviral particles, BSL-2 or higher containment and institutional biosafety committee (IBC) approval are generally required.
• PPE: Lab coat, gloves, and safety glasses. Use nuclease-free technique to avoid sample degradation; avoid aerosol generation.
• Engineering controls: Handle viral packaging steps in a certified biosafety cabinet. Use sealed rotors for centrifugation of viral supernatants.
• Decontamination: Fresh 0.5–1% sodium hypochlorite (10% household bleach) for surfaces and spills; follow with ethanol/water wipe-down. Dispose of contaminated consumables as biohazard waste per local regulations.
• Incompatibilities: Avoid nucleases (DNase/RNase), extreme pH, and repeated freeze–thaw cycles.
• First aid (overview): Skin/eye contact with buffers—rinse with water. Inhalation of aerosols—move to fresh air. Ingestion—seek medical advice. Always consult the SDS for authoritative instructions.

Note: No hazard statements are specified for this catalog item; always consult the SDS and institutional biosafety policies for definitive guidance.

Solvent Selection

Applicability note

• This product is a nucleic acid construct; conventional organic solvent selection is not typically relevant. Work is performed in aqueous, nuclease-free buffers.

General/literature guidance (not item-specific)

• Preferred media: Nuclease-free water or low-salt buffers (e.g., 10 mM Tris-HCl, pH 7.5–8.5) with optional 0.1–1 mM EDTA for chelation of divalent cations that promote nuclease activity.
• Compatibility: Avoid phenol, chloroform, or alcohols except during purification workflows. High ionic strength can affect transfection efficiency; exchange into the buffer required by your downstream protocol.
• Filtration/sterility: 0.22 µm sterile filtration of buffers is standard for cell culture.
• Additives: For long-term DNA stability during working storage, 1–10 mM Tris and EDTA at low micromolar to millimolar levels are commonly used (literature); confirm compatibility with downstream enzymes and cells.

If you require organic solvents for associated steps (e.g., precipitation)

• Ethanol/isopropanol: Used for DNA precipitation and wash; completely remove before resuspension to avoid cytotoxicity.
• Glycerol: Sometimes included at small percentages for freeze-thaw resilience of enzymes, not DNA itself.

Always match buffer conditions to your transfection/transduction method and cell type.

Storage and Reconstitution

Item-specific (from Product Data)

• Storage conditions: Store at −80°C.
• Shipping: Dry ice packs + cold packs.
• Formulation/Buffer and concentration: Not specified for this item; refer to CoA/Spec Sheet.

General handling guidance (not item-specific)

• Upon receipt: Verify package integrity and keep frozen. Minimize time at ambient temperature.
• Aliquoting: Thaw once on ice, mix gently (avoid vortexing to prevent shearing), and make single-use aliquots in nuclease-free tubes to avoid repeated freeze–thaw cycles.
• Reconstitution: If supplied lyophilized, reconstitute in nuclease-free water or low-salt Tris buffer (e.g., 10 mM Tris-HCl, pH 7.5–8.5). If supplied in solution, maintain the provided buffer; avoid unnecessary buffer exchanges.
• Short-term storage: 2–8°C for hours to ≤1 day during handling; keep on ice during setup.
• Long-term storage: −80°C. Avoid frost-free freezers and repeated freeze–thaw.
• Nuclease control: Use RNase/DNase-free plastics and reagents; clean work area to prevent contamination.

Documentation

• For exact buffer composition, DNA concentration, antibiotic marker, and sequence information, consult the CoA/Spec Sheet for SKU P743889.

Research use note

• For research use only (per Product Data). Not for human or veterinary use.

Structure and Identity

Item-specific (from Product Data)

• Product: pLenti-ARID2-sgRNA (lentiviral sgRNA construct)
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• CID: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not applicable to nucleic acid constructs; not assigned.
• SMILES: Not applicable to nucleic acid constructs; not assigned.
• Molecular formula / MW: Not specified for this item; refer to CoA/Spec Sheet.

General/literature description (for context; not item-specific)

• Molecule type: DNA plasmid or lentiviral transfer vector encoding a single-guide RNA (sgRNA) targeting the ARID2 locus.
• Typical architectural features of pLenti sgRNA vectors: 5′/3′ LTRs (self-inactivating in many modern designs), a U6 or H1 RNA polymerase III promoter driving the sgRNA, an sgRNA scaffold (tracrRNA fusion), and often a selection marker (e.g., puro) and/or reporter cassette. Specific elements for this item are not provided.
• 2D “structure” in words: circular double-stranded DNA vector (plasmid) comprising modular transcriptional cassettes and lentiviral backbone elements; includes a short (~20 nt) guide region specifying genomic target (sequence not provided).

Synthetic Utility

Not typically applicable

• As a biological genetic tool, pLenti-ARID2-sgRNA is not used as a reagent in small-molecule synthesis or classic organic transformations.

General molecular biology utility (not item-specific)

• Cloning workflows: Vectors of this class can be subcloned, re-cloned with alternative guides, or combined with donor templates for HDR studies using standard cloning techniques (restriction/ligation, Gibson assembly, Golden Gate). Specific cloning sites and sequences for this item are not provided.
• Functional studies: Useful for perturbation experiments to map regulatory networks and chromatin remodeling dependencies.

For chemical synthesis applications, refer to appropriate small-molecule building blocks or catalysts rather than nucleic acid constructs.

Target Specificity

Item-specific (from Product Data)

• Antigen/Target name: Not specified for this item; refer to CoA/Spec Sheet.
• Epitope/Guide sequence: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity / genome build alignment: Not specified for this item; refer to CoA/Spec Sheet.
• Clone/Isotype (if antibody): Not applicable.

Note

• The product name suggests an sgRNA directed toward the ARID2 locus; however, definitive target sequence, strand, PAM context, and genome build are not provided in the Product Data and must be confirmed from the CoA/Spec Sheet before use.

Häufig gestellte Fragen

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.