pLenti-UBD-sgRNA

Cat. No.: P751478

Spezifikationen

Product Name

pLenti-UBD-sgRNA

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at -80°C

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Stored at -20 ℃, valid for at least 6 months;Stored at -80 ℃, valid for at least one year.

Application Protocols

No tested applications or protocols are provided for this SKU.

• Manufacturer Applications (verbatim): Not provided in the listing.
• General note: Implementation of any sgRNA/lentiviral workflows should follow institutional approvals and biosafety requirements. Consult the vector map/sequence and the SDS/CoA for item-specific guidance.

For detailed, stepwise procedures or recommended conditions, request the official protocol sheet specific to this SKU.

Biological Roles

This product name suggests an sgRNA expression vector associated with “UBD.” Item-specific biological targeting details are not provided.

• General context (literature):
  • sgRNA vectors enable CRISPR systems to address specific genomic loci defined by the sgRNA spacer sequence. The biological role is determined entirely by that spacer and the effector nuclease (e.g., Cas variants).
  • If “UBD” refers to the ubiquitin D context or a promoter label, note that ubiquitin-related pathways govern protein turnover via the ubiquitin–proteasome system; any specific gene or promoter usage must be confirmed from the vector map/documentation.

No target gene, promoter identity, or spacer sequence is specified for this item; consult the CoA/Spec Sheet for definitive biological context.

Buffer Applications

Buffer systems are not typically a primary feature for a plasmid listing. However, general considerations apply (literature):

• Storage buffers: Plasmid DNA is commonly stored in nuclease-free water or TE buffer to enhance stability. Exact buffer for this SKU is not specified; refer to CoA/Spec Sheet.
• Working solutions: Maintain ionic conditions compatible with downstream enzymes or delivery reagents; avoid divalent cations if nuclease contamination is a concern.

No item-specific buffer recipes or pH ranges are provided for this product.

Green Alternatives

While plasmid DNA itself does not raise typical green chemistry solvent concerns, upstream/downstream workflows can be made more sustainable:

• Greener choices (general, literature):
  • Use non-ethidium bromide DNA stains (e.g., SYBR Safe-type) to reduce mutagenic waste.
  • Prefer aqueous, room-temperature workflows and mini-prep kits with reduced plastic and greener buffers where possible.
  • Consolidate shipments to reduce dry ice consumption; ensure proper long-term storage to minimize reshipments.
• Tradeoffs:
  • Non-ethidium stains may alter detection sensitivity; validate for your gel system.
  • Low-plastic workflows may increase manual handling time; balance sustainability with data quality.

Sustainability note: This SKU ships on dry ice + cold packs to preserve integrity; coordinate delivery to avoid re-shipments and reduce cold-chain impact.

Pharmaceutical Uses

This product is designated “For research use only.” It is not an excipient or an approved component for pharmaceutical manufacture or clinical use.

• Formulation/CMC context: Not applicable—no pharmacopeial monograph or GMP status is indicated.
• Typical research-only roles (literature): Plasmid vectors are used in discovery biology, target validation, and screening platforms. Any translational or manufacturing use would require dedicated GMP-grade materials and regulatory clearance, which are not implied for this SKU.

Physical Properties

Item-specific physicochemical data are not provided for this plasmid product.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight/size: Not specified for this item; refer to CoA/Spec Sheet. (Plasmids commonly range ~6–15 kb, literature.)
• pH of storage buffer: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Plasmid DNA is water-soluble; readily soluble in aqueous buffers (TE, nuclease-free water) (literature).
• A260/A280 ratio: Typical purity assessment 1.8–2.0 for DNA (literature; not a spec for this SKU).
• Extinction coefficient: Sequence-dependent; not specified for this item.
• Melting behavior: DNA duplex denaturation depends on GC content, ionic strength, and length (literature); item-specific Tm not provided.

All quantitative specifications (concentration, buffer composition, residual salts/solvents, endotoxin level, host strain) are not specified for this item; refer to CoA/Spec Sheet.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• What to expect for high-quality plasmid reagents (general, literature):
  • Defined vector map and sequence, antibiotic resistance marker identity, and total plasmid length (kb).
  • Purity indicators such as A260/A280 ~1.8–2.0 and minimal genomic RNA/DNA contamination.
  • Supercoiled fraction is often preferred for cloning and high-efficiency transfection; percentage not specified for this item.
  • Endotoxin levels: For sensitive cell types, low-endotoxin prep is preferred; item-specific endotoxin is not specified.
• Documentation:
  • Request the Certificate of Analysis (CoA) or Spec Sheet for this SKU for: concentration, buffer composition, sequence/map, antibiotic marker, host strain, and QC tests performed (restriction digest profile, sequencing spot-check, sterility).
• Stabilizers/additives: Not specified for this item; refer to CoA/Spec Sheet. Any RNase/DNase-free buffer components (e.g., Tris, EDTA) should be disclosed in the documentation if applicable.

Reaction and Applications

No manufacturer application notes were provided for this item. The following are general, high-level roles of lentiviral sgRNA plasmids (literature), without procedural detail:

• sgRNA expression cassette: Supports expression of a CRISPR single guide RNA for targeted genome editing or interference when co-delivered with appropriate CRISPR effector systems.
• Lentiviral backbone utility: Lentiviral transfer vectors are widely used for stable delivery of RNA expression cassettes into dividing and non-dividing mammalian cells (subject to institutional biosafety approval). This SKU’s specific backbone features are not provided.
• Cloning and sequence modification: sgRNA spacer insertion is typically accomplished by standard cloning techniques (e.g., annealed oligos into a defined site), but the exact method depends on the vector map; consult the product’s sequence documentation.

Important: This section is for conceptual context only. Do not undertake genetic modification without proper approvals, training, and compliance. For this SKU’s exact application scope and steps, refer to the official vector map, sequence, and CoA/Spec Sheet.

Reaction Conditions

Detailed experimental conditions are not provided for this SKU and are beyond the scope of this listing. The following high-level considerations are general (literature) and are not procedural instructions:

• DNA handling: Maintain nuclease-free conditions and avoid repeated freeze–thaw.
• Enzymatic steps (if applicable): Reaction buffers and temperatures are enzyme-specific; consult the enzyme manufacturer’s recommendations when conducting standard cloning or verification steps. No item-specific conditions are provided.
• Delivery to cells: Any use involving lentiviral systems requires institutional approvals and appropriate biosafety level containment; follow institutional policies. No protocols are provided here.

Safety and Handling

• GHS/SDS: Not specified for this item; refer to SDS for authoritative hazard classification, pictograms, and H-statements.
• Signal word / H-statements / pictograms: Not specified for this item; refer to SDS.
• General risk profile (literature): Purified plasmid DNA is generally considered non-hazardous; handle using standard molecular biology aseptic practices. If used to generate lentiviral particles, that activity typically requires institutional approval and BSL-2 containment; follow local biosafety guidelines.
• Handling:
  • Use clean, nuclease-free tools and PPE (lab coat, gloves, eye protection) to prevent contamination and exposure.
  • Avoid repeated freeze–thaw cycles to maintain DNA integrity.
• Incompatibilities:
  • Avoid nucleases (DNase), microbial contamination, extreme pH (<6 or >9 without suitable buffer), and high temperatures.
  • Avoid oxidizing/chaotropic contaminants that degrade DNA (e.g., residual bleach in vessels).
• First aid (overview; defer to SDS):
  • Skin/eye contact with buffer: Rinse with water. If irritation persists, seek medical advice.
  • Ingestion/inhalation: Unlikely exposure in normal use; seek medical advice per SDS.
• Waste: Dispose of DNA-containing waste per institutional biosafety procedures. Decontaminate surfaces with appropriate DNA-degrading solutions where required.

Solvent Selection

This product is an aqueous biological macromolecule (DNA). Organic solvent selection is not typically applicable.

• Compatibility (general, literature):
  • DNA is soluble and stable in nuclease-free water or low-salt buffers (e.g., TE). Alcohols (ethanol/isopropanol) are used for precipitation, not storage.
  • Avoid strong chaotropes or organic solvents for routine handling; they can denature or precipitate DNA.
• If downstream workflows require mixing with transfection reagents or buffers, follow the reagent vendor’s compatibility guidance.

For solvent-focused comparisons (e.g., HPLC, spectroscopy), this section is not applicable to a plasmid DNA product.

Storage and Reconstitution

• Storage conditions (item-specific): Store at -80°C.
• Shipping (item-specific): Shipped on dry ice packs + cold packs.
• Formulation and reconstitution: Not specified for this item; refer to CoA/Spec Sheet for buffer composition, concentration, and any reconstitution steps if supplied lyophilized.
• Handling recommendations (general, literature):
  • Thaw on ice and mix gently to avoid shearing; aliquot upon first thaw to minimize freeze–thaw cycles.
  • Use nuclease-free tubes and tips. Avoid repeated access to the stock vial to limit contamination risk.

If long-term storage at -20°C is considered, verify compatibility with the supplied buffer and the manufacturer’s guidance; default for this item remains -80°C as specified.

Structure and Identity

This product is a lentiviral CRISPR support reagent named pLenti-UBD-sgRNA. It is expected to be a circular double‑stranded DNA plasmid or lentiviral vector backbone designed to express a single guide RNA (sgRNA), nominally under a promoter associated with “UBD” nomenclature. Item‑specific sequence-level details are not provided.

• Item-specific identifiers:
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not applicable to plasmid DNA; not specified for this item.
  • SMILES: Not applicable to macromolecular DNA; not specified for this item.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general, literature):
  • Double-stranded, covalently closed circular DNA (plasmid) or lentiviral transfer vector backbone containing sgRNA expression cassette (literature).
  • Typical elements in sgRNA vectors may include: a small RNA polymerase III promoter (e.g., U6) driving sgRNA, cloning sites for protospacer insertion, antibiotic resistance marker, and bacterial origin of replication (literature). Specific elements for this item are not provided.
• 2D structure description (general):
  • Plasmid maps represent discrete functional modules arranged linearly around a circular backbone (promoter → sgRNA scaffold → selection marker → ori, etc.). Exact map for this SKU is not specified; consult the CoA/Spec Sheet.

Note: Where exact sequence, length (kb), and features are critical, request the vector map and sequence file from the product documentation.

Synthetic Utility

For chemists and molecular biologists, the “synthetic utility” of a lentiviral sgRNA plasmid is in genetic construct assembly rather than small-molecule synthesis.

• Functional modules (general, literature):
  • sgRNA scaffold region accepting a user-defined spacer for locus targeting.
  • Bacterial propagation elements (ori, antibiotic resistance) enabling amplification in E. coli.
  • Lentiviral transfer backbone supporting packaging when combined with compatible helper plasmids under approved conditions.
• Workflow implications:
  • Choice of cloning method (restriction/ligation, Gibson-type assembly, or Golden Gate) depends on the vector’s provided sites; exact sites for this SKU are not specified.
  • Sequence verification by Sanger NGS is standard prior to use.

Item-specific cloning sites, selectable markers, and backbone length are not provided; refer to the CoA/Spec Sheet for precise design.

Target Specificity

• Target gene/sequence: Not specified for this item; refer to CoA/Spec Sheet.
• Species/applicability: Not specified for this item.
• Promoter/driver for sgRNA: Not specified for this item.

Note: For sgRNA vectors, specificity is dictated by the spacer sequence. Obtain the exact spacer and vector context from the product documentation.

Häufig gestellte Fragen

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (pLenti-UBD-sgRNA); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.