Potato Dextrose Broth (USP) - BioReagent

Cat. No.: P1528510

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Übersicht

For cultivation of molds and yeasts. Preparation method: Weigh 25.0 g of this product in 1 L of distilled or deionized water (the volume may be scaled up or down proportionally), heat to boiling until completely dissolved, dispense, autoclave at 121°C for 15 minutes, and set aside for use.

Specifications

Spezifikationen & Reinheit

BioReagent

Storage

Room temperature

Verschickt in

Normal

Note

BioReagent

Product Line

Biologische Produkte

Application Protocols

General, literature-based protocol for preparing and using Potato Dextrose Broth (USP). Always follow the instructions on the product label and your institutional SOPs.

• Preparation

  1. Weigh the manufacturer-specified mass of dehydrated medium per liter (check label; literature-typical is ~24 g/L).
  2. Add to purified water and mix until dissolved. Adjust volume to final.
  3. Dispense into suitable vessels (e.g., flasks or bottles with loose caps for autoclaving).
  4. Sterilize at 121 °C for 15 min. Allow to cool to room temperature.
  5. If needed, aseptically add supplements (e.g., antibiotics) after cooling to 45–50 °C.

• Inoculation and incubation

  • Inoculate with a pure culture using sterile technique.
  • For yeasts: Incubate 25–30 °C with shaking (150–200 rpm) to ensure aeration.
  • For filamentous fungi: Incubate 20–30 °C; shaking speed influences pellet vs. dispersed mycelial growth.
  • Monitor turbidity, dry weight, or CFU as required by your method.

• Quality checks

  • Verify pH of prepared medium and clarity before use.
  • Perform growth promotion testing with relevant strains when used in regulated assays.

Item-specific preparation details and acceptance criteria are Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

Potato Dextrose Broth (USP) itself has no intrinsic biological role as a molecule; rather, it serves as a nutritional environment to support microbial metabolism and growth (literature).

• Carbon and energy source: Dextrose (D-glucose) is rapidly utilized via glycolysis and fermentation pathways by yeasts and many fungi, supporting biomass accumulation and, in some species, metabolite production.
• Complex nutrients from potato infusion: Provide amino acids/peptides, vitamins (e.g., B-complex traces), and minerals that facilitate enzyme cofactor availability and cell wall biosynthesis.
• pH context: The characteristically acidic pH (~5–6) suppresses many bacteria while remaining favorable to fungal growth. This is nutritional selectivity rather than antibiotic selection (literature).
• Morphology effects: Nutrient balance and carbon source concentration can influence mycelial vs. pellet growth in filamentous fungi and budding vs. pseudohyphal tendencies in some yeasts (organism-dependent; literature).

Use considerations:

• For targeted physiological studies (e.g., secondary metabolite profiling), researchers may start cultures in PDB to establish biomass, then shift to defined media to control variables.
• Supplementation (e.g., agar for solid media, antibiotics, or specific trace elements) may be applied post-sterilization to steer growth or suppress contaminants (compatibility to be validated in-house).

Buffer Applications

This product is not a buffering agent or a defined buffer system. It is a complex nutrient medium with an inherently mildly acidic pH.

• Buffer capacity: Limited and variable due to undefined composition; not intended for pH stabilization over titration ranges (literature).
• Typical pH of 1× broth: Approximately 5.1–5.6 at 25 °C depending on formulation (literature; not item-specific).

If a defined buffering environment is required (e.g., for enzyme assays or controlled pH fermentation), prepare PDB with an overlay of a defined buffer (such as citrate–phosphate for pH ~5–6) or use a fully defined medium. Always confirm organism tolerance and adjust sterilization protocols accordingly.

Green Alternatives

Although Potato Dextrose Broth (USP) is not a solvent or reagent in the conventional green-chemistry sense, laboratory sustainability considerations still apply.

• Resource efficiency (literature): Prepare only the volume needed to minimize waste; unused sterile medium has limited shelf-life. Consider small-batch preparation or sterile filtration into aliquots to reduce discard volumes.
• Energy use: Autoclaving is energy-intensive. Optimize cycle loads and scheduling to run full loads. Where permitted, consider validated lower-temperature sterilization methods (e.g., filtration) for heat-sensitive supplements.
• Alternative media (functional comparison; literature):
  • Sabouraud Dextrose Broth (SDB): Higher dextrose/peptone content; often similar use for fungi/yeasts; slightly different selectivity and pH.
  • Malt Extract Broth (MEB): Rich in maltose and complex nutrients from malt; commonly used for fungi that prefer maltose-based carbon sources.
  • Yeast Extract–Peptone–Dextrose (YPD) Broth: Optimized for yeasts; very rapid growth but may be less suitable for certain molds.

Trade-offs:

• PDB’s simpler composition (glucose + potato infusion) can limit byproduct formation relative to peptide-rich media, aiding certain metabolite studies.
• Alternative broths may improve growth for specific taxa but can introduce different background nutrients that complicate downstream analyses. Selection should be organism- and application-driven.

Pharmaceutical Uses

No therapeutic or clinical claims apply. In pharmaceutical and personal-care quality control laboratories, Potato Dextrose Broth (USP) is commonly referenced as a compendial culture medium (literature) for:

• Growth promotion testing (GPT) to verify media performance with specified challenge organisms.
• Microbial enumeration of yeasts and molds in non-sterile products and raw materials under pharmacopeial methods.
• Environmental monitoring programs where recovery of fungal bioburden is required.

Notes:

• The “USP” designation in the product name indicates relevance to pharmacopeial methods; however, specific conformity testing for this catalog item is Not specified for this item; refer to CoA/Spec Sheet.
• Laboratories operating under GMP/GLP should perform incoming qualification and suitability tests (e.g., pH, clarity, GPT) on each lot and document results per SOPs.
• When used in regulated contexts, follow the exact medium preparation (mass per liter, pH, sterilization) and incubation parameters specified in the applicable pharmacopeial chapter and your validated method.

Physical Properties

As a dehydrated, undefined culture medium, Potato Dextrose Broth (USP) does not have single-compound physical constants. Where useful, typical literature characteristics of prepared medium are provided with clear labeling.

• Appearance (powder): Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Freely soluble in water; intended to be fully dissolved to make liquid culture medium (literature).
• pH of ready-to-use broth (1×): Typically mildly acidic, often about pH 5.1–5.6 at 25 °C depending on supplier and preparation (literature; not item-specific).
• Osmolality/ionic strength: Not standardized; varies with formulation (literature).
• Density, melting/boiling point, refractive index, logP, pKa: Not applicable to this complex mixture; Not specified for this item; refer to CoA/Spec Sheet.
• Typical preparation concentration: Many USP-aligned PDB formulations use approximately 24 g/L in purified water to yield 1× broth (literature; check label for exact mass per liter).

Practical notes (literature):

• Dissolves readily in room-temperature water with gentle warming/stirring if needed; avoid overheating or prolonged boiling to minimize Maillard browning.
• The acidic pH helps select for fungi by slightly inhibiting many bacteria; however, PDB is not intrinsically selective without added antibiotics or acidifiers.

Quality and Grades

• Item grade: BioReagent (as provided in Product Data). This denotes suitability for biological research workflows, including preparation of microbiological media, where consistent performance and low bioburden are important. It does not imply pharmaceutical excipient status for therapeutic use.
• USP designation: The product name includes (USP), indicating alignment with United States Pharmacopeia usage of Potato Dextrose Broth in compendial microbiological methods (e.g., growth promotion testing, microbial enumeration of fungi/yeasts). Note: Specific compliance testing for this lot is Not specified for this item; refer to CoA/Spec Sheet.
• Media performance: For culture media, quality is typically demonstrated by parameters such as pH of the prepared medium, clarity, and growth promotion testing with specified challenge organisms (e.g., Candida, Aspergillus, Saccharomyces) under defined conditions (literature). For this catalog entry, item-specific acceptance criteria are Not specified; refer to CoA/Spec Sheet.
• Additives/stabilizers: None stated. Not specified for this item; refer to CoA/Spec Sheet.
• Impurities/bioburden: Media-grade specifications often include low background microbial contamination and lot-to-lot consistency; exact limits are Not specified for this item; refer to CoA/Spec Sheet.

Practical guidance:

• Use high-purity water (ASTM Type 1 or 2) to realize the intended USP performance characteristics.
• Verify performance locally with representative strains when media are used for regulated or validated assays.

Reaction and Applications

Chemical reaction use is not applicable. Instead, Potato Dextrose Broth (USP) is used as a nutrient medium in microbiology.

Key laboratory applications (literature; general guidance):

• Cultivation and maintenance of fungi and yeasts, supporting robust mycelial growth and sporulation for many filamentous fungi and proliferation of yeasts (e.g., Saccharomyces, Candida).
• Enumeration of yeasts and molds in foods, cosmetics, and environmental samples, frequently in accordance with compendial standards where PDB (or PDA) is referenced.
• Seed cultures for fermentation: As a pre-culture medium to establish inocula before transfer to production or defined media.
• Growth promotion testing (GPT): Verification that media and test conditions support the growth of specified challenge organisms, as described in pharmacopeial chapters (users should confirm method requirements and perform local GPT; item-specific GPT data are not provided here).

Practical tips (literature):

• Use the manufacturer’s labeled mass-per-liter for preparation; typical PDB is around 24 g/L, but verify on the product label.
• Autoclave at 121 °C for ~15 min. Cool to room temperature before inoculation; if adding heat-labile supplements, cool to 45–50 °C first.
• Incubate at organism-appropriate temperatures (often 20–30 °C for molds; 25–30 °C or 30 °C shaking for yeasts).

Reaction Conditions

Interpreted as preparation and use conditions for media (not chemical reactions). Values below are general literature guidance; always follow the label for this item and your institutional SOPs.

• Reconstitution (typical literature): Dissolve approximately 24 g of dehydrated Potato Dextrose Broth per 1 L of purified water. Mix until fully dissolved.
• pH: Literature target for 1× broth is typically ~5.1–5.6 at 25 °C. Adjust only if required by your method.
• Sterilization: Autoclave at 121 °C for 15 minutes (liquid cycle). Avoid excessive time/temperature to limit caramelization or Maillard reactions.
• Aseptic additions: Add heat-labile supplements (e.g., antibiotics, selective agents) after cooling to 45–50 °C through 0.2 µm sterile filtration.
• Incubation (organism-dependent; literature):
  • Yeasts: 25–30 °C; shaking 150–200 rpm for aeration.
  • Filamentous fungi: 20–30 °C; static or low-speed shaking to control pellet size; baffles can increase shear/aeration.
• Expected performance: Rapid turbidity increase for robust strains within 24–72 h; sporulation for some molds may require transfer to solid potato dextrose agar or light exposure (species-specific).

Item-specific preparation mass, pH targets, and performance criteria are Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

This product is a dehydrated culture medium intended for laboratory use only. While typically considered of low chemical hazard, it is designed to support microbial growth and must be handled with appropriate microbiological practices.

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• Primary hazards: Nutrient medium that can sustain growth of fungi/yeasts and opportunistic contaminants. Risk lies primarily in post-reconstitution contamination and aerosol generation.
• PPE: Laboratory coat, gloves, and safety glasses. Use dust control (avoid inhalation) when handling powder. Employ biological safety measures when working with live cultures.
• Engineering controls: Prepare and dispense sterile media in a biosafety cabinet when working with pathogenic or unknown isolates. Use closed containers during incubation and shaking.
• Incompatibilities: Avoid moisture ingress into the dry powder (caking, contamination). Do not mix with oxidizers/strong acids/bases outside of intended preparation. Keep away from sources of microbial contamination before sterilization.
• Sterilization and decontamination (literature): Autoclave prepared media at 121 °C for ~15 min (or per institutional SOP). Decontaminate spent cultures by autoclaving or validated chemical disinfectants before disposal.
• First aid (general): If dust is inhaled, move to fresh air; rinse eyes/skin with water in case of contact. If ingested, rinse mouth with water. Seek medical attention if symptoms persist. Always consult the SDS for authoritative guidance.
• Waste: Dispose according to institutional biosafety procedures and local regulations for biohazardous waste.

Solvent Selection

This product is a dehydrated culture medium formulated to be used in water.

• Primary solvent: Water (purified/deionized). The medium is designed to dissolve completely in water to produce a clear to slightly opalescent broth after sterilization (literature).
• Polarity/miscibility: Fully water miscible; not intended for use in organic solvents.
• Practical selection notes (literature):
  • Use ASTM Type 1 or 2 water (low TOC, low ionic content) for consistent pH and optical clarity.
  • Avoid hard or chlorinated tap water which can shift pH and introduce inhibitory ions.
  • If selective pressure against bacteria is required, consider sterile supplementation (e.g., antibiotics) after autoclaving and cooling to 45–50 °C; compatibility should be verified per compound.

Because this is not a reaction solvent or a small-molecule reagent, conventional solvent-selection frameworks (e.g., dielectric constant comparisons) are not applicable. Focus on water quality and sterility for optimal performance.

Storage and Reconstitution

• Storage conditions (as provided): Room temperature. Keep the container tightly closed, dry, and protected from humidity to prevent caking and contamination. Avoid long-term exposure to light and elevated temperatures.
• Shipping (as provided): Normal.

Reconstitution and use (literature guidance):

• Dissolve the manufacturer-specified mass per liter of purified water (label value) to prepare 1× broth. Mix thoroughly to complete dissolution.
• Sterilize by autoclaving at 121 °C for 15 minutes unless your validated method specifies otherwise.
• After sterilization, allow to cool to room temperature. Add heat-labile components aseptically at 45–50 °C if required.

Storage after preparation (literature):

• Short-term storage of sterile, unopened broth at 2–8 °C is possible for a limited period (often days to a few weeks), but performance can decline over time; verify clarity and pH before use.
• Avoid repeated temperature cycling. For long-term needs, prepare fresh medium as required.
• Opened containers of dehydrated powder should be promptly resealed and stored desiccated. Consider aliquoting under dry, clean conditions.

Item-specific shelf-life (powder and prepared broth), pH targets, and mass-per-liter are Not specified for this item; refer to CoA/Spec Sheet. Always follow your institutional SOPs and the product label.

Structure and Identity

Potato Dextrose Broth (USP) is a dehydrated, undefined microbiological culture medium. It is not a single chemical substance but a complex mixture formulated for the cultivation of fungi and yeasts.

• Item type: Complex nutrient medium (undefined composition) containing potato infusion components and dextrose (glucose)
• Molecular formula: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• CAS, InChIKey, SMILES: Not applicable to mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (descriptive):
  • Contains dextrose (glucose) as the principal, readily metabolizable carbon source (literature)
  • Potato infusion/solids supply complex nutrients (amino acids, peptides, vitamins, minerals, and growth factors) that support robust mycelial development and yeast proliferation (literature)
• 2D structure (conceptual): There is no single 2D chemical structure; the product consists of multiple organic and inorganic constituents derived from potato infusion plus crystalline D-glucose (literature).

Note: Because composition is deliberately undefined to support broad fungal nutrition, exact amounts of individual components vary by manufacturer specification. Always consult the product label/CoA for lot-specific identity or composition details.

Synthetic Utility

Not applicable in the sense of chemical synthesis. Potato Dextrose Broth (USP) is a biological culture medium rather than a reagent for organic or inorganic transformations.

For researchers seeking metabolites or biotransformations from fungi/yeasts, PDB may serve as a growth phase medium to generate biomass before transferring to production or reaction media tailored for specific biocatalytic steps (literature). In such workflows, synthetic “utility” is indirect—providing robust cell health to support downstream whole-cell or enzyme-mediated transformations.

Target Specificity

Not applicable. This product is not an antibody, probe, or affinity reagent, and therefore has no target specificity parameters (antigen, epitope, clone, isotype). It is a general-purpose nutrient broth intended to support growth of a wide range of fungi and yeasts (literature).