PPP2R4 Antibody - Primärer Antikörper, specific to PPP2R4, Rabbit IgG

Cat. No.: Ab122864

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human PPP2R4 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.

Specifications

Product Name

PPP2R4 Antibody - Primärer Antikörper, specific to PPP2R4, Rabbit IgG

Synonyme

subunit B'' antibody | KIAA0044antibody | MGC2184antibody | Phosphotyrosyl phosphatase activator antibody | PP2A antibody | PP2A phosphatase activatorantibody | PP2A subunit B' antibody | PP2A subunit B' isoform PR53 antibody | PP2A subunit B' PR53 isofor

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Wirtsspezies

Kaninchen

Spezifität

PPP2R4

Immunogen

A synthetic peptide derived from human PTPA (AA 347-358)

Positive Kontrolle

WB: Hep G2, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human thyroid cancer tissue. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: PPP2R4 Antibody (Ab122864)

PPP2R4 Antibody (Ab122864) - Western Blot
All lanes: PPP2R4 Antibody (Ab122864) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 41 kDa
Observed band size: 43 kDa
Exposure time: 4.1 s

Western Blot: PPP2R4 Antibody (Ab122864)

PPP2R4 Antibody (Ab122864) - Western Blot
All lanes: PPP2R4 Antibody (Ab122864) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 41 kDa
Observed band size: 36, 43 kDa
Exposure time: 1.0 s

IHC: PPP2R4 Antibody (Ab122864)

PPP2R4 Antibody (Ab122864) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PPP2R4 Antibody (Ab122864). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: PPP2R4 Antibody (Ab122864)

PPP2R4 Antibody (Ab122864) - IF/ICC

IF analysis of PPP2R4 Antibody (Ab122864) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PPP2R4 Antibody (Ab122864) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

Zugehörige Ziele (menschlich)

PTPA Tchem Serine/threonine-protein phosphatase 2A activator (0 Activities)

Discover Target: PTPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PPP2R4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.