GRADE & PURITYSterile-filtered?Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Ready-to-use?Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors.
BioReagent, sterile-filtered, ready-to-use BioReagent,Ready-to-use,Sterile-filtered for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Aladdin IgG Elution Buffer (1x), pH 2.7, is a ready-to-use, amine-based acidic solution designed for the efficient dissociation and recovery of purified antibodies from Protein A, Protein G, Protein A/G, and Protein L affinity supports. This low-pH buffer enables high-yield elution under denaturing conditions and is suitable for a wide range of antibody-antigen or protein-protein affinity purification systems.
Key Features
High-Efficiency Elution: Effectively dissociates bound antibodies from all major IgG-binding proteins under low-pH conditions.
Ready-to-Use: Pre-diluted 1× solution requires no preparation—ideal for fast and consistent workflows.
Broad Compatibility: Suitable for eluting antibodies in both singleplex and multiplex affinity purification workflows.
Consistent Performance: Pre-adjusted to pH 2.7 to ensure reproducible elution profiles across applications.
Versatile Application: Also effective in antigen-antibody or general protein-protein interaction studies.
Applications
Antibody elution in IgG purification using Protein A, G, A/G, or L resins
Ideal for research and development-scale immunoaffinity purification
Compatible with manual and automated chromatography systems
Suitable for elution in most antigen-antibody affinity purification setups
Instructions for Use
After sample binding and washing steps, apply Aladdin IgG Elution Buffer (1×), pH 2.7, to the affinity resin.
Incubate briefly (typically 1-10 minutes) for complete antibody dissociation.
Collect eluate fractions and immediately neutralize using an appropriate buffer (e.g., 1 M Tris-HCl, pH 9.0) to preserve antibody activity.
For optimal performance, combine with Aladdin’s matching IgG Binding Buffers for a seamless workflow.
Notes
Avoid prolonged exposure of antibodies to low-pH conditions to prevent denaturation.
Neutralize eluted fractions promptly for maximum antibody recovery and stability.
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