GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Suitable for electrophoresis?Electrophoresis grade — low ionic/UV impurities for clean gel runs. Use in agarose/PAGE electrophoresis where impurities distort bands.for PAGE?PAGE grade — purity suited to polyacrylamide gel electrophoresis. Use for protein/nucleic-acid PAGE where reagent purity affects resolution.for Western blot?Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low.5×
BioReagent, for PAGE, for western blot, suitable for electrophoresis, 5× BioReagent,for PAGE,for Western blot,Suitable for electrophoresis for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
This product is designed for protein sample preparation in SDS-PAGE electrophoresis. Its novel formulation ensures a neutral pH after boiling, preventing protein degradation. It features fast thawing from –20°C and eliminates unpleasant odors by replacing traditional reducing agents (e.g., β-mercaptoethanol or DTT) with an advanced odorless alternative. Key Features 1.Prevents Degradation: Neutral pH after boiling avoids protein degradation, ideal for phosphorylated proteins. 2.Rapid Settlement: Samples sink quickly to the bottom of wells without floating. 3.Fast Thawing: Rapid melting from –20°C without viscosity. 4.Odorless: Contains a novel reducing agent—no β-mercaptoethanol or DTT, no unpleasant smell. Protocol 1.Add 1 volume of loading buffer to 4 volumes of protein sample (1:4 ratio) and mix thoroughly. 2.Heat the mixture in a boiling water bath for 5–10 minutes. 3.Centrifuge at high speed for 5 minutes. Use the supernatant for electrophoresis. 3.1Note: If no insoluble debris is observed after adding loading buffer, brief centrifugation is sufficient to collect the mixture. 3.2If condensation forms on the tube lid after heating, gently flick the tube to mix condensation with the sample before centrifugation to ensure uniform loading concentration. Precautions 1.Gels loaded with this buffer may exhibit yellowing or streaks in silver staining due to the reducing agent. 2.Not suitable for non-denaturing gel electrophoresis as it contains denaturing agents. 3.Wear a lab coat and disposable gloves for safety. 4.For research use only.
Specifications
Synonyms
SDS-PAGE Sample Loading Buffer
Specifications & Purity
BioReagent, for PAGE, for western blot, suitable for electrophoresis, 5×
Stability And Storage
Store at 2-8℃ short term (3 months). Store at -20℃ long term (12 months).
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, for PAGE, for Western blot, Suitable for electrophoresis
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
