QUALITÄT & REINHEITCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.KO Validation?Knockout-validated — antibody specificity confirmed using knockout cells/tissue. Use when you need strong evidence the antibody binds only its target.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
Carrier Free, KO Validation, ExactAb™, Azide Free, Validated, See COA Azide Free,Carrier Free,ExactAb™,KO Validation,Validated für empfindliche chromatographische und analytische Workflows, die minimale Basislinieninterferenzen erfordern.
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Lagerung & Versand
Store at -20°C,Avoid repeated freezing and thawing Versand Ice chest + Ice pads. Genaue Spezifikationen finden Sie im chargenspezifischen COA.
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Qualitätsdokumente
SDS, COA, Datenblatt und Spezifikationsblatt stehen zum Download bereit. Chargenspezifische COA über Chargennummernsuche abrufbar.
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Literaturbeleg
In 0 peer-reviewten Publikationen zu Chromatographie, organischer Synthese und Kreuzkupplungsreaktionen zitiert.
Übersicht
Rabbit anti Human PSMD9 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on. Application:
WB: 0.01-5 µg/mL IHC: 5-20 µg/mL Flow Cytometry: 5-20 µg/mL per 10^6 cells in 100 µl volume
IF/ICC: 2.5 µg/mL Protein Function: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
Spezifikationen
Product Name
PSMD9 Antibody - Primärer Antikörper, specific to PSMD9, Rabbit IgG
Carrier Free, KO Validation, ExactAb™, Azide Free, Validated, See COA
Wirtsspezies
Kaninchen
Spezifität
PSMD9
Immunogen
Recombinant protein of human PSMD9 (AA 2-223).
Positive Kontrolle
WB: A549, HeLa, Hep G2, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human liver, liver cancer, glioma, lung cancer, breast cancer, prostate tissues. Flow Cytometry: Human SiHa cells. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
Azide Free, Carrier Free, ExactAb™, KO Validation, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Antigen affinity purified + Protein A purified
Konzentration
See COA
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
PSMD9 Antibody (Ab123714) - Western Blot All lanes: PSMD9 Antibody (Ab123714) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 23, 28 kDa Exposure time: 23.7 s
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Lung cancer Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - Flow Cytometry Human SiHa cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100, then stained with 20 µg/mL PSMD9 Antibody (Ab123714) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.
PSMD9 Antibody (Ab123714) - IF/ICC IF analysis of PSMD9 Antibody (Ab123714) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PSMD9 Antibody (Ab123714) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
IHC
5-20 µg/mL
WB
0.01-5 µg/mL
Flow Cytometry
5-20µg/mL per 10^6 cells in 100 µl volume
IF/ICC
2.5 µg/mL
Dokumentation
📋 Sicherheitsdatenblatt (SDS)
Umfassendes Dokument zu Gefahren, Handhabung, Lagerung und Einhaltung regulatorischer Vorschriften.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Bewertungen
Kundenbewertungen
Häufig gestellte Fragen
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What does KO Validation mean?
KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PSMD9 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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