Pullulanase Activity Assay Kit (DNS, Micro Method)

Cat. No.: P1527316

GRADE & PURITY BioReagent

Übersicht

Pullulanase is a hydrolytic enzyme widely distributed in microorganisms, animals, and plants. It specifically cleaves the α‑1,6‑glycosidic bonds at the branch points of starch, glycogen, and their derivatives. This enzyme was initially used in fundamental research on starch structure. Since the 1970s, its applications have gradually expanded to starch deep‑processing fields such as starch syrup production, beer brewing, and alcohol production, marking a transition from laboratory research to industrial‑scale manufacturing. This kit employs the DNS colorimetric method for the determination of pullulanase activity. The principle is as follows: Pullulanase catalyzes the hydrolysis of the substrate pullulan to generate reducing sugars. The reducing sugars react with 3,5‑dinitrosalicylic acid (DNS) under boiling water bath conditions to form a brown‑red amino compound, which exhibits a characteristic absorption peak at 540 nm. Within the established linear range, the increase in absorbance at 540 nm is directly proportional to the amount of reducing sugars generated. By continuously monitoring the rate of increase in absorbance, the pullulanase activity in the sample can be calculated.

Specifications

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Detection instrument

Microplate reader

Detection wavelength

540nm

Lagerung und Versand

Storage

Store at 2-8°C

Verschickt in

Wet ice