Pyruvate oxidase, CAS No.9001-96-1

CAS: 9001-96-1 Cat. No.: P774057 EC Number: 232-644-5

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥15 U/mg enzyme powder; ≥50 U/mg protein

Accession #

P37063

Bioactivity

≥15 U/mg enzyme powder; ≥50 U/mg protein

Overview

Source: Microorganism 
Isoelectric point: 5.0 
Michaelis constant: 5.3×10^-4 M (Pyruvate) 
Optimum pH: 7.0-7.5                     Fig. 1
Optimum temperature: 40℃~50℃                     Fig. 3
pH Stability: 6.0~7.0 (37℃, 60min)                     Fig. 2
Thermal stability: <45℃ (pH 6.5, 30min)                     Fig. 4
Inhibitors: Cu2+, Ni2+, Zn2+, NaN3, Proclin, SDS, Triton X-100 
Effect of various chemicals:                     Table 1
Reaction:

Reaction:
  

Specifications

Product Name

Pyruvate oxidase, CAS No.9001-96-1

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥15 U/mg enzyme powder; ≥50 U/mg protein

Bioactivity

≥15 U/mg enzyme powder; ≥50 U/mg protein

Accession #

P37063

Predicted molecular weight

68 kDa (SDS-PAGE)

CAS

9001-96-1

Enzyme Commission Number

EC 1.2.3.3

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥15 U/mg enzyme powder; ≥50 U/mg protein

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (5 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit will convert one micromole of pyruvate to acetylphosphate per min at pH 6.7at 37℃.