GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥20U/mg enzyme powder; ≥90U/mg protein
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥20U/mg enzyme powder; ≥90U/mg protein ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
3α-Hydroxysteroid Dehydrogenase catalyze the conversion of 3-ketosteroids into 3α-hydroxy compounds. 3α-Hydroxysteroid dehydrogenase plays an important role in the inactivation of androgen DHT, and can convert DHT into 3α-androstanediol with weak androgen activity, which can be used in the research of hirsutism.
Reaction
Product description
Appearance:
White amorphous powder
Source:
Microorganism
Enzyme Commission Number:
EC 1.1.1.50
CAS Number:
9028-56-2
Storage temperature:
-20℃
Activity:
≥20U/mg enzyme powder; ≥90U/mg protein
Unit definition:
One unit will oxidate one micromole of androsterone per min at pH 8.25 at 25℃.
Properties
Stability:
Stable at -20℃ for at least five years
Molecular weight:
28 kDa (SDS-PAGE)
Isoelectric point:
6.4
Michaelis constant:
3.0×10⁻⁵M (Androsterone)6.0×10⁻⁶M (NAD⁺)
Optimum pH:
10.5
{Fig. 1}
Optimum temperature:
50℃-60℃
{Fig. 3}
pH Stability:
4.5-10.5 (30℃, 20hr)
{Fig. 2}
Thermal stability:
< 50℃ (pH 7.2, 20min)
{Fig. 4}
Inhibitors:
Cu²⁺,Fe²⁺,Zn²⁺,NEM,Proclin,SDS
Effect of various chemicals:
{Table 1}
Table 1. Effect of Various Chemicals on HSD
[The enzyme dissolved in 50mM Tris-HCl buffer, pH 7.5 (10U/ml) was incubated with each chemical at 37℃ for 2hr.]
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Specifications
Product Name
Recombinant 3α-Hydroxysteroid Dehydrogenase (HSD), CAS No.9028-56-2
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥20U/mg enzyme powder; ≥90U/mg protein
Biochemical and Physiological Mechanisms
Along with the 3 alpha-hydroxysteroid dehydrogenase and 3-oxo-reductase activities towards a variety of cis or trans fused A/B ring steroids, it also reduces several xenobiotic carbonyl compounds, including a metyrapone-based class of insecticides, to th
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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