QUALITÄT & REINHEITRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.5 mg/mL
KLH-conjugated synthetic peptide encompassing a sequence within human ACAA2.
Positive Kontrolle
WB: HEK293, Hep G2, HeLa, NIH/3T3 and C6 cell lysates. IHC: Rat liver tissue. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
ExactAb™, Recombinant, Validated
Isotyp
Rabbit IgG
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Immunogen affinity purified
Konzentration
0.5 mg/mL
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Recombinant ACAA2 Antibody (Ab087071) - Western Blot All lanes: Recombinant ACAA2 Antibody (Ab087071) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 80.0 s
Recombinant ACAA2 Antibody (Ab087071) - IF/ICC Immunohistochemical analysis of Recombinant ACAA2 Antibody (Ab087071) staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Recombinant ACAA2 Antibody (Ab087071) - IF/ICC IF analysis of Recombinant ACAA2 Antibody (Ab087071) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant ACAA2 Antibody (Ab087071) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
IHC
1/50-1/200
IF/ICC
1/200
Dokumentation
📋 Sicherheitsdatenblatt (SDS)
Umfassendes Dokument zu Gefahren, Handhabung, Lagerung und Einhaltung regulatorischer Vorschriften.
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