Recombinant Bub1 Antibody - Primärer Antikörper, specific to BUB1, Rabbit IgG

Cat. No.: Ab091854

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human Bub1 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Specifications

Product Name

Recombinant Bub1 Antibody - Primärer Antikörper, specific to BUB1, Rabbit IgG

Synonyme

Bub1 | BUB1 budding uninhibited by benzimidazoles 1 homolog | BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) | BUB1_HUMAN | BUB1A | BUB1L | Budding uninhibited by benzimidazoles 1 (yeast homolog) | Budding uninhibited by benzimidazoles 1 hom

Spezifikationen & Reinheit

Recombinant, ExactAb™, Validated, See COA

Wirtsspezies

Kaninchen

Spezifität

BUB1

Immunogen

A synthesized peptide derived from human Bub1 (AA 1-200)

Positive Kontrolle

WB: K562 and HeLa cell lysates.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant Bub1 Antibody (Ab091854)

Recombinant Bub1 Antibody (Ab091854) - Western Blot
All lanes: Recombinant Bub1 Antibody (Ab091854) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 122 kDa
Observed band size: 128 kDa
Exposure time: 61.7 s

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

Zugehörige Ziele (menschlich)

BUB1 Tchem Mitotic checkpoint serine/threonine-protein kinase BUB1 (0 Activities)

Discover Target: BUB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen