Recombinant CD19 Antibody (PE) - Primärer Antikörper, specific to Mouse CD19

Cat. No.: Ab213880

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Maus

Isotype

Rat IgG2a

Clone Number

P99G09

Conjugation

PE

Übersicht

Recombinant CD19 Antibody, clone P99G09, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD19 Antibody (PE) - Primärer Antikörper, specific to Mouse CD19

Synonyme

Antibody deficiency due to defect in CD19 antibody | Antibody deficiency due to defect in CD19, included antibody | AW495831 antibody | B lymphocyte antigen CD19 antibody | B lymphocyte surface antigen B4 antibody | B-lymphocyte antigen CD19 antibody | B-

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Wirtsspezies

Rat

Spezifität

Mouse CD19

Immunogen

Mouse CD19-expressing K562 human erythroleukemia cells

Positive Kontrolle

Flow Cytometry: C57BL/6 mouse splenocytes

Konjugation

PE

Anregung (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Note

ExactAb™, Validated

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein A purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: Recombinant CD19 Antibody (PE) (Ab213880)

Recombinant CD19 Antibody (PE) (Ab213880) - Flow Cytometry
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD19 Antibody (PE) (Ab213880) at 0.04μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: Recombinant CD19 Antibody (PE) (Ab213880)

Recombinant CD19 Antibody (PE) (Ab213880) - Flow Cytometry
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD19 Antibody (PE) (Ab213880) at 0.04μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

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CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)

Discover Target: CD19

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)