GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.5 μL/test
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b
Wirtsspezies
Maus
Spezifität
Human CD3ε
Immunogen
Human thymocytes
Positive Kontrolle
Flow Cytometry: Human peripheral blood lymphocytes
Konjugation
APC
Anregung (nm)
Red Laser(633nm)
Emission (nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
Note
ExactAb™, Recombinant, Validated
Lichterketten-Typ
kappa
Verfahren zur Reinigung
Protein A purified
Konzentration
5 μL/test
Product Line
Biologische Produkte
Lagerung und Versand
Storage
Store at 2-8°C,Protected from light,Do not freeze
Verschickt in
Wet ice,Do not freeze
Stabilität und Lagerung
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (APC) (Ab230565) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (APC) (Ab180309).
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (APC) (Ab230565) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.
Anwendung
Anwendung
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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