GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)See COA
Carrier Free,Recombinant,ExactAb™,Azide Free,Validated,High Performance,≥95%(SDS-PAGE),See COA
Biochemische und physiologische Mechanismen
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide co
Wirtsspezies
Rat
Spezifität
CD4
Immunogen
BALB/c mouse thymocytes
Positive Kontrolle
Flow: C57BL/6 Mouse spleen cells
Konjugation
Unconjugated
Note
Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Lichterketten-Typ
kappa
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein A purified
Konzentration
See COA
Hinweis
This product can replace Ab094744.
Lagerung und Versand
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Flow Cytometry analysis of C57BL/6 Mouse splenocytes labelling CD4 with Recombinant CD4 Antibody (Ab222901) (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Ab141774) (left panel) and detected with AF 647 - conjugated affinipure goat anti-Rat IgG.
Flow Cytometry analysis of C57BL/6 Mouse splenocytes labelling CD4 (red) with Recombinant CD4 Antibody (Ab222901) at 0.2 μg/mL for 1 hour at 4°C and detected with AF 647 - conjugated affinipure goat anti-Rat IgG. Blue - Rat IgG Isotype Control Antibody (Ab141774). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD4 Antibody (Ab222901) - SEC
The purity of Recombinant CD4 Antibody (Ab222901) is more than 95% verified by HPLC.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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