GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)See COA
Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Host species
Rat
Specificity
CD8 beta
Immunogen
Mouse thymocytes
Positive Control
Flow: C57BL/6 mouse splenocytes
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Flow Cytometry analysis of C57BL/6 mouse splenocytes cells labelling CD8b with Recombinant CD8b Antibody (Ab222903) (right panel) at 1.0 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Ab141774) (left panel) and detected with AF 647 - conjugated affinipure goat anti - Rat IgG.
Flow Cytometry analysis of C57BL/6 mouse splenocytes cells labelling CD8b (red) with Recombinant CD8b Antibody (Ab222903) at 1.0 μg/mL for 1 hour at 4°C and detected with AF 647 - conjugated affinipure goat anti - Rat IgG. Blue - Rat IgG Isotype Control Antibody (Ab141774). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD8b Antibody (Ab222903) - SEC
The purity of Recombinant CD8b Antibody (Ab222903) is more than 95% verified by HPLC.
Application
Application
Dilution info
Flow Cytometry
≤0.5 µg per million cells in 100 µl volume
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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