GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.3 mg/mL
ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Rabbit anti Human DDIT3 Antibody, Recombinant, could be used for WB, IHC and so on.
Specifications
Product Name
Recombinant DDIT3 Antibody - Primärer Antikörper, specific to DDIT3, Rabbit IgG
Synonyme
C/EBP zeta | C/EBP-homologous protein | C/EBP-homologous protein 10 | CCAAT/enhancer-binding protein homologous protein | CHOP | CHOP-10 | DDIT-3 | Growth arrest and DNA damage-inducible protein GADD153 | C/EBP-homologous protein 10 | C/EBP-homologous pro
Spezifikationen & Reinheit
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Biochemische und physiologische Mechanismen
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
Wirtsspezies
Kaninchen
Spezifität
DDIT3
Immunogen
A synthetic peptide derived from human DDIT3 (AA 145-160)
Positive Kontrolle
WB: K562, THP-1 and HEK293 cell lysates. IHC: Human breast cancer tissue.
Konjugation
Unconjugated
Note
ExactAb™, Recombinant, Validated
Isotyp
Rabbit IgG
Lichterketten-Typ
kappa
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein A purified
Konzentration
0.3 mg/mL
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Recombinant DDIT3 Antibody (Ab099377) - Western Blot All lanes: Recombinant DDIT3 Antibody (Ab099377) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 19 kDa Observed band size: 28 kDa Exposure time: 7.6 s
Recombinant DDIT3 Antibody (Ab099377) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant DDIT3 Antibody (Ab099377). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Anwendung
Anwendung
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Bewertungen
Kundenbewertungen
Häufig gestellte Fragen
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant DDIT3 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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