GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.See COA
Recombinant, ExactAb™, KD Validation, Validated, See COA ExactAb™,KD Validation,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Rabbit anti Human E2F1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant E2F1 Antibody - Primärer Antikörper, specific to E2F1, Rabbit IgG
Recombinant, ExactAb™, KD Validation, Validated, See COA
Biochemische und physiologische Mechanismen
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF
Wirtsspezies
Kaninchen
Spezifität
E2F1
Immunogen
A synthetic peptide derived from human E2F1 (AA 365-400)
Positive Kontrolle
WB: HeLa, A375, NIH/3T3 and C6 cell lysates. IHC: Human ovary carcinoma tissue. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
ExactAb™, KD Validation, Recombinant, Validated
Isotyp
Rabbit IgG
Lichterketten-Typ
kappa
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein A purified
Konzentration
See COA
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Recombinant E2F1 Antibody (Ab100782) - Western Blot All lanes: Recombinant E2F1 Antibody (Ab100782) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 66, 74 kDa Exposure time: 3.8 s
Recombinant E2F1 Antibody (Ab100782) - Western Blot All lanes use Recombinant E2F1 Antibody (Ab100782) at 1/1000 dilution for 1 hour at room temperature.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782).
Recombinant E2F1 Antibody (Ab100782) - IHC Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using Recombinant E2F1 Antibody (Ab100782).
Anwendung
Anwendung
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Bewertungen
Kundenbewertungen
Häufig gestellte Fragen
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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