Recombinant E2F6 Antibody - Primärer Antikörper, specific to E2F6, Rabbit IgG

Cat. No.: Ab100799

QUALITÄT & REINHEIT Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

Kaninchen

Reactive species

Mensch

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human E2F6 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.

Spezifikationen

Product Name

Recombinant E2F6 Antibody - Primärer Antikörper, specific to E2F6, Rabbit IgG

Synonyme

E2F binding site modulating activity protein | E2F transcription factor 6 | E2F transcription factor 6 isoform 1 | EMA | MGC111545 | Transcription factor E2F6 | E2F6_HUMAN | E2F 6 antibody | E2F 6 | E2F binding site modulating activity protein antibody |

Spezifikationen & Reinheit

Recombinant, ExactAb™, Validated, See COA

Wirtsspezies

Kaninchen

Spezifität

E2F6

Immunogen

A synthetic peptide derived from human E2F6 (AA 100-281)

Positive Kontrolle

WB: K562, Jurkat, Hep G2 and HeLa cell lysates.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant E2F6 Antibody (Ab100799)

Recombinant E2F6 Antibody (Ab100799) - Western Blot
All lanes: Recombinant E2F6 Antibody (Ab100799) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 100.0 s

Anwendung

Anwendung

Dilution info

WB

1/500-1/2000

Zugehörige Ziele (menschlich)

E2F6 Tbio Transcription factor E2F6 (0 Activities)

Discover Target: E2F6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F6 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.