GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Rabbit anti Human EPF Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
Recombinant EPF Antibody - Primärer Antikörper, specific to HSPE1, Rabbit IgG
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
Wirtsspezies
Kaninchen
Spezifität
HSPE1
Immunogen
A synthetic peptide derived from human Cpn10 (AA 92-102).
Positive Kontrolle
WB: Mouse liver, Rat kidney, K562, HeLa, Hep G2, NIH/3T3 and C6 lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
Konjugation
Unconjugated
Note
ExactAb™, Recombinant, Validated
Isotyp
Rabbit IgG
Lichterketten-Typ
kappa
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein A purified
Konzentration
See COA
Product Line
biological_products
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Recombinant EPF Antibody (Ab101869) - Western Blot All lanes: Recombinant EPF Antibody (Ab101869) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 11 kDa Observed band size: 12 kDa Exposure time: 9.2 s
Recombinant EPF Antibody (Ab101869) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant EPF Antibody (Ab101869). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant EPF Antibody (Ab101869) - IF/ICC IF analysis of Recombinant EPF Antibody (Ab101869) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant EPF Antibody (Ab101869) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Anwendung
Anwendung
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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