GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Accession #
Q13641-1
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Immobilized Human TPBG at 1 μg/mL (100 μL/well) can bind TPBG Antibody with a linear range of 0.1-2 ng/mL
Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, ≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Purity ≥95% (SDS-PAGE) Endotoxin level ＜0.1 EU/μg Function 5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling (2). 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5). Human 5T4 is a 420 amino acid (aa) protein that includes a 31 aa signal peptide, a 324 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD, human 5T4 shares 78% and 77% aa sequence identity with mouse and rat 5T4, respectively. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain. 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles. Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1. In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8.
50 - 70 kDa, under reducing conditions; 50 -70 kDa, under non-reducing conditions.
Molekültyp
Protein
Lagerung und Versand
Form
Lyophilized
Rekonstitution
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder
Recombinant Human 5T4 Protein (rp142398) - SDS-PAGE 3 μg/lane of Recombinant Human 5T4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 50 - 70 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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