Recombinant Human Amyloid Precursor Protein, ≥95% (SDS-PAGE)

Cat. No.: rp142805

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, ≥95%(SDS-PAGE)

Expression System

HEK293

Accession #

P05067

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<0.1 EU/μg

Bioactivity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is < 0.24 nM.

Overview

Purity
≥95% (SDS-PAGE)
Endotoxin level
＜0.1 EU/μg
Function
Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).

Specifications

Product Name

Recombinant Human Amyloid Precursor Protein, ≥95% (SDS-PAGE)

Synonyms

ABPP | Alzheimer disease amyloid protein | Amyloid precursor protein | Amyloid-beta A4 protein | APP | APPI | Cerebral vascular amyloid peptide | CVAP | PN-II | PreA4 | Protease nexin-II | A4 amyloid protein | A4_HUMAN | AAA | ABETA | ABPP | AD1 | AICD-50

Grade

ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, His Tag

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, ≥95%(SDS-PAGE)

Purity

≥95% (SDS-PAGE)

Bioactivity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is < 0.24 nM.

Endotoxin Concentration

<0.1 EU/μg

Expression System

HEK293

Species

Human

Amino Acids

1-688 aa

Sequence

MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLHHHHHH

Protein Tag

C-His

Accession #

P05067

Predicted molecular weight

79 kDa

SDS-PAGE

100 - 125 kDa, under reducing conditions; 100 - 125 kDa, under non-reducing conditions.

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

SDS-PAGE: Recombinant Human Amyloid Precursor Protein (rp142805)

Recombinant Human Amyloid Precursor Protein (rp142805) - SDS-PAGE
3 μg/lane of Recombinant Human Amyloid Precursor Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 100 - 125 kDa.

References

1. Xiaoming Zha, Doriano Lamba, Lili Zhang, Yinghan Lou, Changxu Xu, Di Kang, Li Chen, Yungen Xu, Luyong Zhang, Angela De Simone, Sarah Samez, Alessandro Pesaresi, Jure Stojan, Manuela G. Lopez, Javier Egea, Vincenza Andrisano, Manuela Bartolini.  (2015)  Novel Tacrine–Benzofuran Hybrids as Potent Multitarget-Directed Ligands for the Treatment of Alzheimer’s Disease: Design, Synthesis, Biological Evaluation, and X-ray Crystallography.  JOURNAL OF MEDICINAL CHEMISTRY,      [PMID:26632651] [10.1021/acs.jmedchem.5b01119]

2. Xu Shao, Chao Wang, Chaoli Wang, Mengyao Bai, Tongtong Hou, Xin Wang, Chaoren Yan, Ping Guan, Xiaoling Hu.  (2024)  Novel photocatalytic carbon dots: efficiently inhibiting amyloid aggregation and quickly disaggregating amyloid aggregates.  Nanoscale,  16  (16): (8074-8089).  [PMID:38563405] [10.1039/D3NR06165E]