Recombinant Human CNDP2 Protein

Cat. No.: rp188331

GRADE & PURITY Carrier Free, His Tag, ≥90%(SDS-PAGE), expressed in E. coli; See COA

Accession #

Q96KP4

Protein Tag

N-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Specifications

Product Name

Recombinant Human CNDP2 Protein

Synonyme

Cytosolic non-specific dipeptidase | CNDP dipeptidase 2 | CN2 | CPGL | HsT2298 | PEPA | Glutamate carboxypeptidase-like protein 1 | Peptidase A | Cytosolic non-specific dipeptidase | CNDP dipeptidase 2 | CN2 | CPGL | HsT2298 | PEPA | Glutamate carb

Note

Carrier Free, His Tag

Spezifikationen & Reinheit

Carrier Free,His-Tag,≥90%(SDS-PAGE),expressed in E. coli; See COA

Biochemische und physiologische Mechanismen

CNDP Dipeptidase 2 (CNDP2), is a cytosolic, non-specific dipeptidase which is a part of the peptidase M20A protein family. CNDP2 is a secreted peptidase homologous to M20 peptidases. CNDP2 expresses through all adult and fetal tissue, though, an isoform m

Endotoxin-Konzentration

<1.0 EU/μg

Aminosäuren

2-475 aa

Sequenz

MHHHHHHAALTTLFKYIDENQDRYIKKLAKWVAIQSVSAWPEKRGEIRRMMEVAAADVKQLGGSVELVDIGKQKLPDGSEIPLPPILLGRLGSDPQKKTVCIYGHLDVQPAALEDGWDSEPFTLVERDGKLYGRGSTDDKGPVAGWINALEAYQKTGQEIPVNVRFCLEGMEESGSEGLDELIFARKDTFFKDVDYVCISDNYWLGKKKPCITYGLRGICYFFIEVECSNKDLHSGVYGGSVHEAMTDLILLMGSLVDKRGNILIPGINEAVAAVTEEEHKLYDDIDFDIEEFAKDVGAQILLHSHKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVPNMTPEVVGEQVTSYLTKKFAELRSPNEFKVYMGHGGKPWVSDFSHPHYLAGRRAMKTVFGVEPDLTREGGSIPVTLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRYNYIEGTKMLAAYLYEVSQLKD​

Beitritt #

Q96KP4

Voraussichtliches Molekulargewicht

53.7 kDa

SDS-PAGE

50.2 kDa, under reducing conditions; 46.7 & 52.3 kDa, under non-reducing conditions.

Molekültyp

Protein

Product Line

Biologische Produkte

Lagerung und Versand

Konzentration

expressed in E. coli; See COA

Rekonstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at -20℃ for 6 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

SDS-PAGE: Recombinant Human CNDP2 Protein (rp188331)

Recombinant Human CNDP2 Protein (rp188331) - SDS-PAGE 

3 μg/lane of Recombinant Human CNDP2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 50.2 kDa under reducing conditions and 46.7 & 52.3 kDa under non-reducing conditions.

Application Protocols

The following are general, literature-based protocols suited to recombinant CNDP2 and are not item-specific. Optimize for your system.

• CNDP2 activity assay (HPLC readout)

  1. Prepare assay buffer: 25 mM HEPES, 150 mM NaCl, 1 mM MnCl2, pH 7.5.
  2. Make substrate solution (e.g., dipeptide at 0.5–2 mM).
  3. Equilibrate to 30°C. Initiate reaction by adding enzyme (final 50–200 nM).
  4. At defined times, quench aliquots with 1–2% formic acid or 50 mM EDTA (post-sampling) and place on ice.
  5. Analyze by RP-HPLC/LC–MS to quantify substrate depletion and amino acid products.

• Immobilized CNDP2 microreactor (His-tag)

  1. Charge Ni-NTA resin; equilibrate with HEPES/NaCl buffer containing 0.5–1 mM MnCl2.
  2. Load enzyme; wash to remove unbound protein; verify activity with a small test reaction.
  3. Pass substrate solution through at controlled residence time; collect effluent and analyze.

• SDS-PAGE identity check

  1. Mix protein with Laemmli buffer; heat mildly (e.g., 60–70°C, 5 min) to preserve activity in parallel samples.
  2. Run alongside MW standards; stain to assess purity and apparent MW.

Note: Exact enzyme concentration and specific activity for this item are not specified; refer to CoA/Spec Sheet and titrate empirically.

Biological Roles

• Overview (literature; not item-specific)

  • CNDP2 encodes cytosolic non-specific dipeptidase 2, a metallopeptidase (M20 family) that hydrolyzes dipeptides into constituent amino acids. It contributes to intracellular dipeptide turnover, including histidine-containing dipeptides such as carnosine.

• Enzymatic mechanism and cofactors

  • Requires divalent metal ion(s) for catalysis; activity is typically enhanced by Mn2+ or Co2+ and inhibited by chelators like EDTA (literature). The catalytic site coordinates metal ion(s) to activate a water nucleophile for peptide bond cleavage.

• Cellular and biochemical context

  • Localized primarily in the cytosol (literature). Functions in amino acid recycling, nitrogen economy, and modulation of dipeptide pools that can serve as pH buffers, antioxidants, or osmolytes.

• Research relevance

  • Model enzyme for studying metal-dependent peptidase catalysis, substrate specificity in dipeptide metabolism, and regulation of intracellular histidine dipeptides.

Note: The biological roles described above summarize general knowledge of human CNDP2 and are not functional claims for a specific lot. Verify activity and cofactor dependence experimentally under your assay conditions.

Buffer Applications

CNDP2 work is typically performed in aqueous buffers optimized for metallopeptidase stability and activity.

• Common buffer systems (general guidance; not item-specific)

  • HEPES, 20–50 mM, pH 7.2–7.6; 100–200 mM NaCl; optional 0.5–2 mM MnCl2/CoCl2 for activity assays.
  • Tris-HCl, 25–50 mM, pH 7.5–8.0; 150 mM NaCl; note Tris pH varies with temperature.
  • Phosphate, 10–50 mM, pH 7.0–7.4; be mindful of potential interactions with divalent cations at higher concentrations.

• Additives

  • Glycerol (5–20%) for cryoprotection and improved stability during storage and freeze–thaw.
  • Detergents: avoid unless necessary; if used, choose mild nonionic types at ≤0.01–0.05% and assess activity impact.

• Incompatible buffer components for activity assays

  • Chelators (EDTA, EGTA), strong reducing agents at high concentrations, heavy-metal contaminants, and high phosphate with certain metal ions.

• Practical recipes (illustrative)

  • Activity buffer: 25 mM HEPES, 150 mM NaCl, 1 mM MnCl2, pH 7.5; 25–37°C.
  • Storage buffer: 25 mM HEPES, 300 mM NaCl, 10–20% glycerol, pH 7.5; aliquot and store at −20°C or below.

Exact buffer composition for this item is not specified; consult the CoA/Spec Sheet.

Green Alternatives

This recombinant protein is used in aqueous media and does not generally require organic solvents, aligning well with green chemistry principles.

• Greener aspects (general)

  • Aqueous buffers at ambient temperature minimize VOC use and energy consumption.
  • Enzymatic peptide hydrolysis offers high chemoselectivity, reducing need for protecting groups and harsh reagents typical of chemical hydrolysis.

• Practical guidance to maintain a green profile

  • Favor HEPES or phosphate buffers prepared in-house from concentrated stocks; avoid single-use plastic where feasible (use low-bind, reusable glass when compatible).
  • Limit disposal of metal-containing buffers; when metal ions (e.g., Mn2+/Co2+) are used, collect waste appropriately.
  • Avoid DMSO except for substrate/inhibitor stocks; keep final organic cosolvent ≤1–5% where possible.

• Comparison (general; not item-specific)

  • Enzymatic dipeptide cleavage vs. acid/base hydrolysis
    • Conditions: neutral pH, ambient temperature vs. strong acids/bases and heat
    • Selectivity: high vs. low
    • Waste: minimal aqueous salt solutions vs. acidic/basic neutralization salts

Overall, employing CNDP2 in buffered water is already a greener alternative to traditional chemical peptide bond cleavage strategies.

Pharmaceutical Uses

This product is supplied for research use only.

• Non-therapeutic, research-focused roles

  • Reference enzyme for developing and qualifying analytical assays that monitor dipeptide hydrolysis in bioprocess samples (e.g., LC–MS methods), or to benchmark inhibitor potency in discovery workflows.
  • Potential tool enzyme in process development studies exploring enzymatic peptide trimming under mild aqueous conditions.

• Excipient/formulation status

  • No pharmacopeial excipient role is indicated or implied for this product.

• Regulatory note

  • Not for use in humans or clinical diagnostics. Any application in regulated environments would require independent qualification/validation by the user.

Item-specific regulatory certifications, viral safety, host cell protein/DNA levels, and endotoxin specifications are not provided for this item; refer to the CoA/Spec Sheet if available.

Physical Properties

• Item-specific physical properties

  • Appearance/formulation: Not specified for this item; refer to CoA/Spec Sheet.
  • Concentration: Not specified for this item; refer to CoA/Spec Sheet.
  • Extinction coefficient (A280), pI, and exact MW of the tagged construct: Not specified for this item; refer to CoA/Spec Sheet.
  • Solubility: Recombinant proteins are generally soluble in compatible physiological buffers; specific solubility for this lot is not specified; refer to CoA/Spec Sheet.

• General/literature properties for human CNDP2 (not item-specific)

  • Monomeric polypeptide length ~475 aa; calculated MW ~52–53 kDa; His-tag adds ~0.8–3 kDa depending on design (literature)
  • Isoelectric point (theoretical): typically in the neutral range for CNDP2 sequences; precise value depends on construct (literature)
  • Solubility: water-soluble; prefers near-neutral pH buffers; stability often enhanced with salt (100–300 mM NaCl) and cryoprotectants (glycerol) (general protein practice)
  • Thermal behavior: activity measurable at 25–37°C; denaturation on heating; freeze–thaw can reduce activity (literature/general)

• Non-applicable classical small-molecule properties

  • Boiling point, melting point, logP, refractive index, SMILES/InChIKey are not applicable to proteins.

Note: Use the CoA/Spec Sheet for any lot-specific protein concentration, buffer composition, and molecular mass confirmation (SDS-PAGE/SEC/MS).

Quality and Grades

• Item-specific grade

  • Carrier Free: Supplied without carrier proteins (e.g., BSA, gelatin). Advantages: precise mass measurements, cleaner assay backgrounds, compatibility with functional assays and labeling chemistries. Considerations: may adsorb to plastics at low µg/mL concentrations—minimize surface losses using low-bind plastics or include inert protein only if assay permits.
  • His Tag: Engineered polyhistidine tag enables immobilized metal affinity chromatography (IMAC) purification and facile detection/capture on Ni2+/Co2+ resins. Tag position/length is not specified for this item; refer to CoA/Spec Sheet.

• What this means for performance

  • Lower risk of carrier-related interference in enzymatic and binding assays.
  • His-tag may slightly alter pI or surface properties; in most cases, does not impede activity, but confirm empirically for sensitive applications.

• Typical QC for recombinant enzymes (informational; not item-specific)

  • SDS-PAGE for purity and approximate MW; activity assay with dipeptide substrates; identity by mass spectrometry or Western blot (anti-His, anti-CNDP2).

• Not provided for this item (consult CoA/Spec Sheet)

  • Purity percentage, endotoxin level, host system, residual imidazole, and exact buffer composition are not specified for this item; refer to CoA/Spec Sheet.

Reaction and Applications

As a recombinant metallopeptidase, CNDP2 is broadly useful in enzymology, metabolism studies, and biocatalysis.

• Enzymology and metabolism (literature; not item-specific)

  • CNDP2 (CN2) hydrolyzes various dipeptides, including histidine-containing substrates such as carnosine (β-alanyl-L-histidine), releasing free amino acids. Activity depends on divalent metal cofactors and near-neutral pH.
  • Useful for kinetic characterization (Km, kcat) across substrate panels to map specificity and catalytic preferences.

• Assay development and standards

  • Reference enzyme for validating dipeptidase activity assays (HPLC/LC–MS monitoring of dipeptide cleavage; colorimetric/fluorometric amino acid detection via derivatization reagents such as OPA or fluorescamine).
  • Tool for evaluating inhibitor/activator structure–activity relationships (SAR) in the M20 peptidase family.

• Biocatalysis and processing

  • Selective hydrolysis of dipeptide bonds under mild aqueous conditions; potential utility in peptide trimming workflows and analytical peptide mapping.

• Protein interaction and capture

  • His-tag enables immobilization on Ni2+/Co2+-NTA resins for flow-reactor formats or pulldown experiments (ensure retention of activity post-immobilization).

• Practical tips (general)

  • Remove residual chelators; include requisite metal ions if needed for catalysis.
  • Assess activity at 25–37°C; pre-equilibrate enzyme and substrate in assay buffer; avoid repeated freeze–thaw by aliquoting.

Manufacturer application notes: Not provided for this item; see sections above for common research uses.

Reaction Conditions

General guidance for in vitro CNDP2 activity assays and preparative hydrolyses (literature; not item-specific):

• Buffer and pH

  • 20–50 mM HEPES or Tris buffers, pH 7.2–8.0. Activity is typically optimal near neutral to slightly basic conditions.

• Metal cofactors

  • Include 0.5–2 mM MnCl2 or CoCl2 to ensure maximal activity when required; avoid EDTA/EGTA. Some M20 peptidases can bind Zn2+ but may show altered activity; determine empirically.

• Temperature and time

  • 25–37°C for kinetic assays. Reaction times vary from minutes to hours depending on enzyme and substrate concentrations.

• Typical concentrations

  • Enzyme: 5–500 nM for kinetic assays; up to µM levels for preparative hydrolysis.
  • Substrate: 10 µM to several mM depending on solubility and detection method.

• Detection

  • Quench with acid or chelator (only after sampling is complete), then analyze by HPLC/LC–MS for dipeptide depletion and amino acid formation; or derivatize amino acids (e.g., OPA) for fluorescence.

• Controls

  • No-enzyme, heat-inactivated enzyme, and no-metal controls to confirm enzymatic, metal-dependent turnover.

Note: Exact optimal conditions depend on the specific construct and buffer composition. This item’s formulation and activity specs are not provided; consult CoA/Spec Sheet and perform pilot screens.

Safety and Handling

• GHS classification and hazard statements for this item: Not specified for this item; refer to SDS.

• Anticipated hazard profile (general guidance for recombinant proteins; not item-specific)

  • Typically considered low hazard for laboratory handling; avoid inhalation, ingestion, and skin/eye contact.
  • Handle as a potential sensitizer; repeated exposure to protein aerosols can cause allergic responses.

• Personal protective equipment (PPE)

  • Lab coat, disposable nitrile gloves, and safety glasses.
  • Use a biosafety cabinet when preparing aerosols/lyophilizates or when sterility is required.

• Handling cautions specific to metalloproteases (general)

  • Avoid chelators (e.g., EDTA, EGTA) when catalytic activity must be preserved; these can strip essential metal cofactors.
  • Avoid proteases and harsh surfactants that may degrade or denature the protein.

• First-aid (general)

  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical attention if irritation persists.
  • Inhalation: Move to fresh air; seek medical attention if symptoms develop.
  • Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

• Storage incompatibilities and stability

  • Store at −20°C as specified for this item; avoid repeated freeze–thaw cycles.
  • Keep away from strong acids/bases, oxidizers, and proteolytic enzymes.

Always consult the product SDS and institutional EHS policies for authoritative guidance.

Solvent Selection

For recombinant CNDP2, solvent selection centers on aqueous buffer systems compatible with metallopeptidase structure and activity.

• Buffer and solvent compatibility (general guidance)

  • Preferred: Near-neutral pH aqueous buffers (e.g., 20–50 mM HEPES, Tris, or phosphate; pH ~7.0–8.0).
  • Ionic strength: 100–300 mM NaCl often enhances solubility and stability without impairing activity (literature/general protein practice).
  • Additives: 5–20% glycerol for cryoprotection; 0.5–2 mM MnCl2 or CoCl2 may be used when activity assays require metal reconstitution; avoid chelators.
  • Detergents: If needed for solubility, use mild nonionic agents at low levels (e.g., 0.01% Tween-20); evaluate impact on activity.

• Avoid

  • EDTA/EGTA and phosphate at high concentrations if metal reconstitution is desired (metal chelation/precipitation risks).
  • Organic cosolvents >10% (v/v), strong acids/bases, and denaturants (urea/guanidine) except for analytical unfolding.

• When to choose one buffer over another

  • Tris-HCl (pH 7.5–8.0): Good for activity screens; note Tris is temperature-dependent in pKa.
  • HEPES (pH 7.2–7.6): Excellent pH stability across temperature variations.
  • Phosphate (pH 7.0–7.4): Common storage buffer; monitor compatibility with divalent cations.

Note: Exact buffer used for this item is not specified; consult the CoA/Spec Sheet for formulation details.

Storage and Reconstitution

• Item-specific storage and shipping

  • Storage: Store at −20°C; avoid repeated freezing and thawing (per Product Data).
  • Shipping: Ice chest + ice pads (per Product Data).

• Reconstitution (general guidance; not item-specific)

  • If supplied lyophilized: Reconstitute with sterile buffer (e.g., 20–50 mM HEPES or Tris, 150–300 mM NaCl, pH 7.4–7.8). Aim for ≥0.1–1 mg/mL to minimize adsorption in carrier-free formulations.
  • If supplied in solution: Gently mix by inversion; avoid vigorous vortexing or foaming.
  • Optional additives for stability: 10–20% glycerol; 1–5 mM DTT or TCEP only if reducing conditions are required and confirmed compatible with activity. Avoid EDTA/EGTA if catalytic activity is desired.

• Aliquoting and freeze–thaw management

  • Prepare single-use aliquots in low-bind tubes; snap-freeze in liquid nitrogen or a dry ice/ethanol bath before transferring to −20°C or below.
  • Thaw on ice; keep on ice during use. Do not refreeze thawed aliquots.

• Long-term storage

  • For multi-month storage, consider −80°C in suitable buffer with cryoprotectant. Assess activity over time.

Exact formulation, concentration, and stabilizers for this item are not specified; consult the CoA/Spec Sheet.

Structure and Identity

Recombinant Human CNDP2 Protein (carrier-free, His-tag) corresponding to human cytosolic non-specific dipeptidase 2 (CNDP2, also known as CN2), a metallopeptidase of the M20 family.

• Item-specific identifiers

  • SKU: rp188331
  • Product name: Recombinant Human CNDP2 Protein (Carrier Free, His Tag)
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight (as supplied): Not specified for this item; refer to CoA/Spec Sheet.
  • Sequence length, construct boundaries, and precise tag position: Not specified for this item; refer to CoA/Spec Sheet.

• Literature identity (general, not item-specific)

  • Gene: CNDP2 (HGNC: 21547); UniProtKB: Q96KP4 (literature)
  • Typical length: ~475 amino acids; calculated MW ~52–53 kDa for the untagged monomer (literature)
  • Oligomeric state: commonly homodimeric metallopeptidase (M20 family) (literature)
  • Functional domains: metal-binding catalytic center with characteristic HxH/E motifs of M20 enzymes (literature)

• Structural features (general description)

  • Proteinaceous macromolecule; no simple SMILES or InChIKey representation applicable.
  • Contains an N-terminal or C-terminal polyhistidine affinity tag for IMAC purification and detection (tag position not specified for this item).
  • 2D/3D description: globular enzyme fold typical of M20 peptidases with a catalytic metal-binding pocket; surface-exposed His-tag segment enables Ni2+/Co2+ chelate binding (literature).

Synthetic Utility

Although CNDP2 is a biological macromolecule rather than a small-molecule reagent, it can serve as a selective biocatalyst.

• Functional capability (literature; not item-specific)

  • Hydrolyzes dipeptides to free amino acids with high chemoselectivity under neutral aqueous conditions. Useful for trimming terminal dipeptides from model substrates in method development and for generating authentic amino acid standards from defined dipeptides.

• Advantages vs. chemical routes

  • Operates at ambient temperatures and physiological pH; avoids strong acids/bases and protecting-group manipulations.
  • High substrate specificity minimizes side reactions and racemization.

• Retrosynthetic perspective

  • For workflows requiring conversion of dipeptides to constituent amino acids, CNDP2 provides a single-step, water-based route. Downstream separation can be achieved by ion exchange or HPLC.

• Practical implementation

  • Immobilize His-tagged CNDP2 on Ni-NTA agarose to create a reusable flow-through reactor for continuous dipeptide hydrolysis; verify retained activity and prevent metal leaching.

Note: Substrate scope and rates depend on sequence context and assay conditions; verify conversion profiles experimentally.

Target Specificity

This section typically documents antibody epitope/clone specificity, which is not applicable to a recombinant enzyme.

• For CNDP2 (general enzymology context; not item-specific)
  • Substrate class: dipeptides, including histidine-containing dipeptides such as carnosine (literature).
  • Specific sequence preferences and turnover rates vary with substrate and assay conditions; determine empirically.

No antibody-related target specificity (antigen, clone, isotype, species reactivity) applies to this product.

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥90% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What is His Tag?

His Tag refers to a recombinant protein product fused with the His affinity tag, suitable for recombinant protein expression and affinity purification. The tag enables one-step affinity purification, immobilization or detection without affecting downstream activity in most cases.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.