Recombinant Human EphB4 Protein

Cat. No.: rp184032

QUALITÄT & REINHEIT Animal Free, Carrier Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Accession #

P54760

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Übersicht

Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor tyrosine kinase family and binds Ephrin-B2. The A and B class Eph proteins have a common structural organization. The human EphB4 cDNA encodes a 987 amino acid precursor that includes a 15 amino acid (aa) signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 427 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM). Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. The ECD of human EphB4 shares 89% aa sequence identity with mouse EphB4 and 42 - 45% aa sequence identity with human EphB1, 2, and 3. EphB4 is expressed preferentially on venous endothelial cells (EC) and inhibits cell-cell adhesion, chemotaxis, and angiogenesis. Opposing effects are induced by signaling through Ephrin-B2 expressed on arterial EC: adhesion, endothelial cell migration, and vessel sprouting. EphB4 signaling contributes to new vascularization by guiding venous EC away from Ephrin-B2 expressing EC. Ephrin-B2 signaling induces arterial EC to migrate towards nascent EphB4 expressing vessels. The combination of forward signaling through EphB4 and reverse signaling through Ephrin-B2 promotes in vivo mammary tumor growth and tumor-associated angiogenesis. EphB4 promotes the differentiation of megakaryocytic and erythroid progenitors but not granulocytic or monocytic progenitors.

Spezifikationen

Product Name

Recombinant Human EphB4 Protein

Synonyme

soluble EPHB4 variant 2 | soluble EPHB4 variant 3 | Tyro11 | Tyrosine-protein kinase receptor HTK | Tyrosine-protein kinase TYRO11 | EC 2.7.10 | EC 2.7.10.1 | EPH receptor B4 | EphB4 | ephrin type-B receptor 4 | hepatoma transmembrane kinase | Htk | HTKep

Note

Animal Free, Carrier Free, His Tag, PBS Only

Spezifikationen & Reinheit

Animal Free, Carrier Free, His Tag, PBS Only, ≥95%(SDS-PAGE)

Bioaktivität

Testing in progress

Endotoxin-Konzentration

<1.0 EU/μg

Expression System

HEK293

Aminosäuren

1-539 aa

Sequenz

MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLAHHHHHH

Protein Tag

C-His

Beitritt #

P54760

Voraussichtliches Molekulargewicht

57.9 kDa

SDS-PAGE

59.4-77.7 kDa, under reducing conditions; 52.4-65.0 kDa, under non-reducing conditions

Molekültyp

Protein

Product Line

Biologische Produkte

Lagerung und Versand

Form

Lyophilized

Rekonstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at -20°C for 1 year. Avoid freeze/thaw cycle.

Bilder

SDS-PAGE: Recombinant Human EphB4 Protein (rp184032)

Recombinant Human EphB4 Protein (rp184032) - SDS-PAGE
3 μg/lane of Recombinant Human EphB4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 59.4-77.7 kDa under reducing conditions and 52.4-65.0 kDa under non-reducing conditions.

Binding Activity​: Recombinant Human EphB4 Protein (rp184032)

Recombinant Human EphB4 Protein (rp184032) - Binding Activity​ 

Immobilized Recombinant Human EphB4 Protein (rp184032) at 2.0 μg/mL can bind Recombinant Human Ephrin-B2 Protein (rp186546) with the EC50 of 52.41 ng/mL.