Recombinant Human IGFBP5 Protein

Cat. No.: rp222575

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His Tag, ≥90%(SDS-PAGE), expressed in HEK293; See COA

Accession #

P24593

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 1.68 µg/mL in the presence of 14 ng/mL rhIGF-II.

Specifications

Product Name

Recombinant Human IGFBP5 Protein

Note

ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag

Spezifikationen & Reinheit

Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His Tag, ≥90%(SDS-PAGE), expressed in HEK293; See COA

Biochemische und physiologische Mechanismen

Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubM

Bioaktivität

Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 1.68 µg/mL in the presence of 14 ng/mL rhIGF-II.

Endotoxin-Konzentration

<1.0 EU/μg

Aminosäuren

1-272 aa

Sequenz

MVLLTAVLLLLAAYAGPAQSLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVEHHHHHH

Beitritt #

P24593

Voraussichtliches Molekulargewicht

29.4 kDa

SDS-PAGE

30.1-35.5 kDa, under reducing conditions; 29.2-34.9 kDa, under non-reducing conditions.

Molekültyp

Protein

Product Line

Biologische Produkte

Lagerung und Versand

Konzentration

expressed in HEK293; See COA

Rekonstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Protein Bioactivity: Recombinant Human IGFBP5 Protein (rp222575)

Recombinant Human IGFBP5 Protein (rp222575) - Protein Bioactivity 

Measured by its ability to inhibit the biological activity of IGF-II (rp147265) on MCF‑7 human breast cancer cells. The ED50 for this effect is 1.68 µg/mL in the presence of 14 ng/mL rhIGF-II.

SDS-PAGE: Recombinant Human IGFBP5 Protein (rp222575)

Recombinant Human IGFBP5 Protein (rp222575) - SDS-PAGE 

3 μg/lane of Recombinant Human IGFBP5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 30.1-35.5 kDa under reducing conditions and 29.2-34.9 kDa under non-reducing conditions.

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥90% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.