Recombinant Keap1 Antibody - Primärer Antikörper, specific to KEAP1, Rabbit IgG

Cat. No.: Ab111970

QUALITÄT & REINHEIT Recombinant, ExactAb™, Validated, High Performance, See COA

Application

IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human Keap1 Antibody, Recombinant, could be used for WB, IHC and so on.

Spezifikationen

Product Name

Recombinant Keap1 Antibody - Primärer Antikörper, specific to KEAP1, Rabbit IgG

Synonyme

Cytosolic inhibitor of Nrf2 antibody | INrf 2 antibody | INrf2 antibody | Keap 1 antibody | KEAP1 antibody | KEAP1_HUMAN antibody | Kelch like ECH associated protein 1 antibody | Kelch like family member 19 antibody | Kelch like protein 19antibody | Kelch

Spezifikationen & Reinheit

Recombinant, ExactAb™, Validated, High Performance, See COA

Biochemische und physiologische Mechanismen

Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its tran

Wirtsspezies

Kaninchen

Spezifität

KEAP1

Immunogen

Recombinant protein of human Keap1 (AA 321-609)

Positive Kontrolle

WB: RAW 264.7, CTLL-2, HeLa, A549, MCF7 and Hep G2 cell lysates. IHC: Human breast cancer tissue.

Konjugation

Unconjugated

Note

ExactAb™, High Performance, Recombinant, Validated

Isotyp

Rabbit IgG

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant Keap1 Antibody (Ab111970)

Recombinant Keap1 Antibody (Ab111970) - Western Blot
All lanes: Recombinant Keap1 Antibody (Ab111970) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 64 kDa
Exposure time: 10.0 s

Western Blot: Recombinant Keap1 Antibody (Ab111970)

Recombinant Keap1 Antibody (Ab111970) - Western Blot
All lanes: Recombinant Keap1 Antibody (Ab111970) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 64 kDa
Exposure time: 20.0 s

IHC: Recombinant Keap1 Antibody (Ab111970)

Recombinant Keap1 Antibody (Ab111970) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Keap1 Antibody (Ab111970). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Anwendung

Anwendung

Dilution info

IHC

1/50 - 1/100

WB

1/1000 - 1/5000

Zugehörige Ziele (menschlich)

KEAP1 Tclin Kelch-like ECH-associated protein 1 (0 Activities)

Discover Target: KEAP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

KEAP1 Tclin Kelch-like ECH-associated protein 1 (1736 Activities)

Discover Target: KEAP1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Keap1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.