GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥90%(SDS-PAGE)≥10 units/mg
Accession #
P13134
Protein Tag
No tag
Expression system
Pichia pastoris
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Specific Activity: ≥10U/mg pro. UNIT DEFINITION: Under the conditions of 25°C and pH 8.0, the amount of enzyme needed to catalyze the conversion of 1 μmol Boc-QR R-pNA into products per minute is one enzyme activity unit (U).
Carrier Free, Bioactive, ActiBioPure™, EnzymoPure™, ≥90%(SDS-PAGE), ≥10 units/mg ActiBioPure™,Bioactive,Carrier Free,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Übersicht
Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α,factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0). The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition. Kex2 Protease Recommended Method: Recommended reaction buffer: 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0. If it is not used immediately after dissolution, it is recommended to use 20mM NaAc-HAc, 2mM Ca2+ (pH 5.0~5.5) buffer to dissolve the lyophilized powder. During the reaction, use 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0 as the reaction diluent. Recommended conditions:
Catalytic activity: Cleavage of -Lys-Arg-|-Xaa- and -Arg-Arg-|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors. Cofactor Ca2+. Note: Binds 3 Ca2+ ions per subunit.
Post-translational: O-glycosylated.
Bioaktivität
Specific Activity: ≥10U/mg pro. UNIT DEFINITION: Under the conditions of 25°C and pH 8.0, the amount of enzyme needed to catalyze the conversion of 1 μmol Boc-QR R-pNA into products per minute is one enzyme activity unit (U).
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Lyophilized powder store at -20℃ for 24 months. Dissolve in buffer (20mM NaAc-HAc, 2 mM Ca2+, pH 5.0~5.5), subpackage and store at -20℃. It is recommended that it should be stored at 2~8℃ after dissolution for no more than 24 hours. Upon receipt, it is re
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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