Recombinant MMP14 Antibody - Primärer Antikörper, specific to MMP14, Rabbit IgG

Cat. No.: Ab115644

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.2 mg/mL

Application

IHC, WB

Host species

Kaninchen

Reactive species

Hamster, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human MMP14 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
Protein Function
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.

Specifications

Product Name

Recombinant MMP14 Antibody - Primärer Antikörper, specific to MMP14, Rabbit IgG

Synonyme

Matrix metallopeptidase 14 (membrane inserted) antibody | Matrix metalloproteinase 14antibody | Matrix metalloproteinase-14 antibody | Membrane type 1 matrix metalloproteinaseantibody | Membrane type 1 metalloprotease antibody | Membrane type matrix metal

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Wirtsspezies

Kaninchen

Spezifität

MMP14

Immunogen

A synthetic peptide derived from human MMP14 (AA 161-174).

Positive Kontrolle

WB: A431, HUVEC and NIH/3T3 cell lysates. IHC: Human breast cancer tissue.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified

Konzentration

0.2 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant MMP14 Antibody (Ab115644)

Recombinant MMP14 Antibody (Ab115644) - Western Blot
All lanes: Recombinant MMP14 Antibody (Ab115644) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 103.0 s

Western Blot: Recombinant MMP14 Antibody (Ab115644)

Recombinant MMP14 Antibody (Ab115644) - Western Blot
All lanes: Recombinant MMP14 Antibody (Ab115644) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 5.0 s

IHC: Recombinant MMP14 Antibody (Ab115644)

Recombinant MMP14 Antibody (Ab115644) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant MMP14 Antibody (Ab115644). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

Zugehörige Ziele (menschlich)

MMP14 Tchem Matrix metalloproteinase-14 (0 Activities)

Discover Target: MMP14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MMP14 Tchem Matrix metalloproteinase 14 (1592 Activities)

Discover Target: MMP14

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant MMP14 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.