Recombinant NEDD4 Antibody - Primärer Antikörper, specific to NEDD4, Rabbit IgG

Cat. No.: Ab117482

QUALITÄT & REINHEIT Recombinant, ExactAb™, Validated, 0.2 mg/mL

Application

WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human NEDD4 Antibody, Recombinant, could be used for WB and so on.
Application
WB: 1/500-1/1000
Protein Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2.

Spezifikationen

Product Name

Recombinant NEDD4 Antibody - Primärer Antikörper, specific to NEDD4, Rabbit IgG

Synonyme

NEDD4-1|Cell proliferation-inducing gene 53 protein|E3 ubiquitin-protein ligase NEDD4|KIAA0093NEDD4-1MGC176705|NEDD4|NEDD-4|Neural precursor cell expressed developmentally down-regulated protein 4|neural precursor cell expressed, developmentally down-regulated 4|receptor-potentiating factor 1|RPF1|Cell proliferation-inducing gene 53 protein antibody|E3 ubiquitin protein ligase Nedd4 antibody|E3 ubiquitin-protein ligase NEDD4 antibody|HECT-type E3 ubiquitin transferase NEDD4 antibody|KIAA0093 antibody|NEDD 4 antibody|NEDD-4 antibody|Nedd4 antibody|Neural precursor cell expressed developmentally down regulated 4 antibody|Neural precursor cell expressed developmentally down-regulated protein 4 antibody|neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody|PIG53 antibody|receptor-potentiating factor 1 antibody|RPF1 antibody|HECT-type E3 ubiquitin transferase NEDD4

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 0.2 mg/mL

Wirtsspezies

Kaninchen

Spezifität

NEDD4

Immunogen

A synthetic peptide derived from human NEDD4 (AA 1286-1306)

Positive Kontrolle

WB: HeLa, A549, U2OS, K562, MCF7, LNCaP, NIH/3T3 and C6 cell lysates.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified

Konzentration

0.2 mg/mL

Hinweis

This product is nearing end-of-life and will be discontinued. The replacement product is Ab117480

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant NEDD4 Antibody (Ab117482)

Recombinant NEDD4 Antibody (Ab117482) - Western Blot
All lanes: Recombinant NEDD4 Antibody (Ab117482) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 149 kDa
Observed band size: 113,128 kDa
Exposure time: 3.9 s

Anwendung

Anwendung

Dilution info

WB

1/500-1/1000

Zugehörige Ziele (menschlich)

MFAP4 Tbio Microfibril-associated glycoprotein 4 (0 Activities)

Discover Target: MFAP4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

NEDD4 Tchem E3 ubiquitin-protein ligase NEDD4 (0 Activities)

Discover Target: NEDD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant NEDD4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.