Recombinant RING2/RING1B/RNF2 Antibody - Primärer Antikörper, specific to RING2/RING1B/RNF2, Rabbit IgG

Cat. No.: Ab125386

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.8 mg/mL

Application

IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human RING2/RING1B/RNF2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function:
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in × chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random × inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.

Specifications

Product Name

Recombinant RING2/RING1B/RNF2 Antibody - Primärer Antikörper, specific to RING2/RING1B/RNF2, Rabbit IgG

Synonyme

EC 2.3.2.27 | HIP2-interacting protein 3 | Huntingtin-interacting protein 2-interacting protein 3 | Protein DinG | RING finger protein 1B | RING finger protein 2 | RING finger protein BAP-1 | RING1b | RING-type E3 ubiquitin transferase RING2 | BAP 1 antib

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 0.8 mg/mL

Wirtsspezies

Kaninchen

Spezifität

RING2/RING1B/RNF2

Immunogen

A synthetic peptide derived from human RING2 / RING1B / RNF2 (AA 118-131)

Positive Kontrolle

WB: HeLa, A549, U-2 OS, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Antigen affinity purified

Konzentration

0.8 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386)

Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - Western Blot
All lanes: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 38 kDa
Observed band size: 41 kDa
Exposure time: 137.7 s

Western Blot: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386)

Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - Western Blot
All lanes: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 38 kDa
Observed band size: 41 kDa
Exposure time: 60.0 s

IHC: Recombinant RING2/RING1B/RNF2 Antibody (Ab125386)

Recombinant RING2/RING1B/RNF2 Antibody (Ab125386) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RING2/RING1B/RNF2 Antibody (Ab125386). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

Zugehörige Ziele (menschlich)

RNF2 Tbio E3 ubiquitin-protein ligase RING2 (0 Activities)

Discover Target: RNF2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant RING2/RING1B/RNF2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.