Recombinant RUNX2 Antibody - Primärer Antikörper, specific to RUNX2, Rabbit IgG

Cat. No.: Ab126131

QUALITÄT & REINHEIT Recombinant, ExactAb™, Validated, See COA

Application

IF/ICC, IHC, WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human RUNX2 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Spezifikationen

Product Name

Recombinant RUNX2 Antibody - Primärer Antikörper, specific to RUNX2, Rabbit IgG

Synonyme

AML3 | CCD | Acute myeloid leukemia 3 protein | CBFA1 | CBF-alpha-1 | CCD1 | CCDAML3 | CLCD | Core-binding factor subunit alpha-1 | core-binding factor, runt domain, alpha subunit 1 | MGC120023 | ML3 | oncogene AML-3 | OSF2 | OSF-2 | osteoblast-specific t

Spezifikationen & Reinheit

Recombinant, ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhanc

Wirtsspezies

Kaninchen

Spezifität

RUNX2

Immunogen

A synthetic peptide derived from human RUNX2 (AA 300-450)

Positive Kontrolle

WB: MDA-MB-231 cell lysate. IHC: Human colon, Human Hodgkin's lymphoma tissue. IF/ICC: Saos-2 cells.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - Western Blot
All lanes: Recombinant RUNX2 Antibody (Ab126131) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 57 kDa
Observed band size: 59 kDa. The RUNX2 protein, also known as Core-binding factor 1, is a transcription factor that plays a crucial role in cell differentiation and skeletal development. Transcription factors with DNA-binding motifs influence migration resulting in the generation of multiple bands.
Exposure time: 8.1 s

IF/ICC: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - IF/ICC
Immunofluorescent analysis of Saos-2 cells, using Recombinant RUNX2 Antibody (Ab126131).

IHC: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - IHC
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using Recombinant RUNX2 Antibody (Ab126131) at 1/300 dilution. 

IHC: Recombinant RUNX2 Antibody (Ab126131)

Recombinant RUNX2 Antibody (Ab126131) - IHC
Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant RUNX2 Antibody (Ab126131).

Anwendung

Anwendung

Dilution info

IHC

1/100-1/500

WB

1/1000 - 1/2000

IF/ICC

1/50 - 1/200

Zugehörige Ziele (menschlich)

RUNX2 Tbio Runt-related transcription factor 2 (0 Activities)

Discover Target: RUNX2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant RUNX2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.