Recombinant SET7 Antibody - Primärer Antikörper, specific to SET7, Rabbit IgG

Cat. No.: Ab127239

QUALITÄT & REINHEIT Recombinant, ExactAb™, Validated, 1.0 mg/mL

Application

WB

Host species

Kaninchen

Reactive species

Maus, Mensch, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Übersicht

Rabbit anti Human SET7 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function:
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.

Spezifikationen

Product Name

Recombinant SET7 Antibody - Primärer Antikörper, specific to SET7, Rabbit IgG

Synonyme

FLJ21193 antibody | H3 K4 HMTase antibody | H3-K4-HMTase SETD7 antibody | H4 lysine 4 specific antibody | Histone H3 K4 methyltransferase antibody | Histone H3 lysine 4 specific methyltransferase antibody | Histone H3-K4 methyltransferase SETD7 antibody |

Spezifikationen & Reinheit

ExactAb™, Validated, Recombinant, 1.0 mg/mL

Wirtsspezies

Kaninchen

Spezifität

SET7

Immunogen

A synthetic peptide derived from human SETD7 (AA 1-30)

Positive Kontrolle

WB: Jurkat, HeLa, U-2 OS and C6 cell lysates.

Konjugation

Unconjugated

Note

ExactAb™, Recombinant, Validated

Isotyp

Rabbit IgG

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Recombinant SET7 Antibody (Ab127239)

Recombinant SET7 Antibody (Ab127239) - Western Blot
All lanes: Recombinant SET7 Antibody (Ab127239) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 41 kDa
Observed band size: 44 kDa
Exposure time: 30.0 s

Anwendung

Anwendung

Dilution info

WB

1/500-1/1000

Zugehörige Ziele (menschlich)

SETD7 Tchem Histone-lysine N-methyltransferase SETD7 (0 Activities)

Discover Target: SETD7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant SET7 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.