Ro 32-7315 , CAS No.219613-02-2

CAS: 219613-02-2 Cat. No.: R1441914 Summenformel: C₂₂H₃₅N₃O₅S Molekulargewicht: 453.60

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Übersicht

Ro 32-7315 is aTNF-α converting enzyme (TACE) inhibitor.

Specifications

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Namen und Kennungen

Molekulargewicht

453.60

Application Protocols

• Item-specific (from Product Data)

  • Tested applications, recommended dilutions, and positive controls: Not specified for this item.

• General usage guidance for small-molecule probes (context only; optimize empirically)

  • Stock preparation: Dissolve in anhydrous DMSO to a convenient concentration (e.g., 10 mM). Vortex and, if necessary, briefly sonicate. Filter through 0.2 µm PTFE to remove particulates if compatible.
  • Plate-based assays: Prepare serial dilutions (e.g., 1:3 or 1:2) in DMSO, then transfer into assay buffer/media to achieve final test concentrations with constant vehicle % across wells.
  • Cellular dosing: Typical starting ranges are 0.01–10 µM with 24–72 h exposure, adjusted per cytotoxicity and target engagement readouts. Always include vehicle controls and a known pathway reference compound when available.
  • Biochemical assays: Pre-incubate compound with enzyme for 5–15 min to assess rapid binding; extend pre-incubation for suspected time-dependent inhibitors and evaluate reversibility by dilution/washout.
  • Data quality: Use triplicates, include inter-plate calibrators, and fit concentration–response curves with appropriate models (four-parameter logistic). Inspect for precipitation or edge effects.

Note: These are general best practices. No application has been validated or certified for Ro 32-7315 in this listing.

Biological Roles

• Item-specific (from Product Data)

  • Biological/target annotations: Not specified for this item.

• Literature/general context (for background; verify for your application)

  • Ro-series small molecules are widely referenced as modulators of protein kinase C (PKC) isoforms, a family of serine/threonine kinases that regulate cell signaling pathways including proliferation, differentiation, secretion, and cytoskeletal dynamics.
  • Chemical probes in this class are used to acutely perturb kinase activity, enabling cause–effect studies of phosphorylation networks and feedback loops. Depending on the compound and system, outcomes may include changes in substrate phosphorylation states, altered gene expression programs, or modulation of ion channel activities (all system-dependent).

• Experimental considerations

  • Off-targets: Many ATP-competitive kinase inhibitors exhibit polypharmacology. It is prudent to profile against a kinase panel or use complementary genetic perturbations (siRNA/CRISPR) to validate mechanism in your system.
  • Time- and concentration-dependence: Establish fast vs slow onset and reversibility with washout studies; some inhibitors display time-dependent binding.
  • Matrix effects: Protein binding and intracellular accumulation can shift apparent potency between biochemical and cellular assays.

• Controls and standards

  • Include positive controls (e.g., a well-characterized PKC modulator) and negative controls (inactive analog or vehicle) to calibrate dynamic range.

Note: No biological role is certified for this specific catalog item; consult peer-reviewed literature and the lot-specific CoA before definitive mechanistic claims.

Buffer Applications

This product is a small-molecule probe, not a buffering agent. However, its successful use in biochemical or cellular assays depends on compatible buffer systems.

• Item-specific (from Product Data)

  • Buffer compatibility/recipes: Not specified for this item; refer to CoA/Spec Sheet.

• General assay buffer guidance (context only)

  • Enzymatic PK/PD assays: Common buffers include HEPES (pH 7.4–7.6) or Tris (pH 7.4–8.0) with Mg2+/ATP as required for kinase reactions; add 0.01–0.05% Tween-20 to reduce nonspecific adsorption if compatible.
  • Cellular assays: Use culture medium appropriate for the model system; dose compound from a concentrated DMSO stock to limit final DMSO to ≤0.1–0.5% v/v unless higher levels are validated.
  • Solubilization aids: If precipitation occurs on dilution, consider stepwise addition, gentle warming (≤37°C), or inclusion of low levels of co-solvent/surfactant validated not to interfere with the readout.

• Practical tips

  • Always run vehicle controls that exactly match the final buffer and co-solvent composition.
  • Verify compound stability in your buffer by LC at the incubation temperature and time.

Note: No specific buffer recipes or pH ranges are claimed for Ro 32-7315 here; optimize conditions empirically for your assay.

Green Alternatives

Because this product is a finished small-molecule probe rather than a process solvent or reagent, “green alternative” considerations mainly concern solvent and handling choices in your assays and sample preparation.

• Greener handling choices (general guidance)

  • Prefer aqueous assay buffers with minimal organic co-solvent. Keep DMSO at ≤0.1–0.5% v/v when assay sensitivity allows.
  • Where feasible, replace DMF/NMP with ethanol or propylene glycol as intermediate co-solvents for stock dilution, balancing solubility and biological compatibility.
  • Use micro-scale formats to reduce chemical use and waste; pre-dispense single-use aliquots to extend shelf-life and minimize repeated thawing.

• Comparison of common co-solvents (general)

  • DMSO: High solvency, widely accepted in screening; not “green” but effective at low percentages.
  • Ethanol/IPA: More benign; moderate solvency; typically tolerated at higher final percentages by cells/enzymes.
  • PEG 400 or Captisol (sulfobutyl ether β-cyclodextrin): Can enhance apparent solubility in vivo/in vitro contexts; assess impacts on assay readouts.

• Waste minimization

  • Use sealed 96-/384-well plates to limit volatilization and worker exposure.
  • Consolidate and segregate organic waste streams; adopt on-instrument low-volume injection methods for LC-MS quantification to reduce solvent consumption.

Note: No greener “alternative compound” to Ro 32-7315 is suggested here; selection of a different probe should be driven by target profile and data quality needs.

Pharmaceutical Uses

• Item-specific (from Product Data)

  • Regulatory status/formulary listings: Not specified for this item.
  • Research Use Note: For research use only

• General context (no therapeutic claims)

  • Role: Ro 32-7315 is supplied as a research-use-only small molecule, typically employed as a chemical probe in discovery biology and assay development. It is not an excipient and is not intended for human or veterinary use.
  • Preformulation considerations (research scale): For in vivo exploratory studies (e.g., preclinical models), researchers often screen solubilization vehicles such as DMSO/PEG 400/saline, ethanol/cremophor/saline, or cyclodextrin complexes. Such uses require independent verification of purity, stability, and safety; none are specified for this item.
  • Analytics: Quantify exposure using validated LC-MS/MS methods with stable isotope-labeled internal standards where possible; confirm compound integrity in formulated vehicles and biological matrices.

• Quality notes

  • In the absence of pharmacopeial monographs or GMP status, this material should not be used for manufacturing or clinical applications.

Disclaimer: Aladdin Scientific supplies this compound strictly for research use. No claims are made regarding clinical safety, efficacy, or suitability for therapeutic applications.

Physical Properties

• Item-specific (from Product Data)

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

• Not specified for this item; refer to CoA/Spec Sheet (do not assume)

  • Melting point (MP)
  • Boiling point (BP)
  • Density
  • Solubility in water/organic solvents
  • LogP, pKa, refractive index, UV cutoff, residual solvents, metal content

• Literature/general expectations for small-molecule bioactives (context only; verify experimentally)

  • Physical state: Many kinase-focused tool compounds are crystalline or amorphous solids at ambient temperature.
  • Solubility handling: Initial dissolution is commonly attempted in anhydrous DMSO (e.g., 10–50 mM stocks), followed by dilution into aqueous buffers with 0.1–1% final DMSO (literature). Actual solubility for Ro 32-7315 is not established here.
  • Hygroscopicity/photolability: Several aromatic heterocycles and conjugated molecules exhibit light sensitivity and slow oxidative degradation; protect from light and air as prudent practice until item-specific data are reviewed.

Practical guidance

• Perform a micro-scale solubility screen (DMSO, DMF, ethanol, acetonitrile, PBS with surfactant) before committing to a working stock.
• Record observed MP by DSC or capillary as an internal QC if the CoA value is unavailable.
• For quantitative work, determine an extinction coefficient by UV-Vis after weighing and verified assay purity; do not assume a literature value without confirmation.

Quality and Grades

• Item-specific (from Product Data)

  • Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

• How to interpret grade/purity (general guidance)

  • Research-use small molecules are typically supplied with an assay purity value (e.g., HPLC area %) and identity confirmation (NMR/HRMS). In the absence of a stated grade here, consult the lot-specific CoA for: analytical method (HPLC/UPLC), detection wavelength(s), purity cutoff, and residual solvent profile.
  • Low UV-absorbing solvent systems and validated gradient methods are preferred for accurate purity of aromatic kinase probes; an orthogonal second method (e.g., ELSD/MS) can help detect non-UV impurities.
  • If the compound requires a stabilizer or specific salt form, it will be declared explicitly on the CoA/label. No stabilizer or salt information is specified for this item.

• Recommended incoming QC (good practice)

  • Verify identity by 1H NMR and MS against literature where available.
  • Confirm purity by HPLC at two wavelengths (e.g., 220/254 nm) or by UPLC-MS.
  • Check water content by Karl Fischer if hygroscopicity is suspected.
  • If your application is biology-facing (enzymology/cellular), consider endotoxin testing of stock solutions when exceptionally low background is required.

Reaction and Applications

• Item-specific (from Product Data)

  • Manufacturer Applications: Not specified beyond category listing in a small-molecule/compound library.

• Research applications (literature/general, not item-certified)

  • Chemical probe usage: Compounds in the Ro-series are frequently used to interrogate protein kinase C (PKC) signaling and related phosphorylation cascades in biochemical and cellular assays. Ro 32-7315 is commonly cited as a PKC-pathway modulator (literature). Verify target engagement and off-target profile for your system before drawing mechanistic conclusions.
  • Assay types: Enzyme inhibition assays (radiometric, TR-FRET, mobility shift), cellular reporter assays (phospho-substrate readouts), and chemoproteomic target validation.

• Practical tips

  • Prepare concentrated DMSO stocks (e.g., 10 mM), filter through 0.2 µm PTFE when feasible, and aliquot to prevent freeze–thaw cycles.
  • Run vehicle-matched controls and a minimal concentration-response (8–12 points, 3-fold dilution) to establish potency and window.
  • Confirm pathway modulation by orthogonal methods (e.g., Western blot for phospho-substrates, kinase panel counterscreens) to mitigate off-target artifacts.

• Not a synthetic reagent

  • Ro 32-7315 is supplied as a finished small-molecule tool; it is not typically used as a reagent or building block in preparative organic synthesis. For synthetic chemistry content, see the Synthetic Utility section.

Reaction Conditions

Not typically applicable. Ro 32-7315 is supplied as a finished bioactive small molecule rather than a synthetic reagent or catalyst.

• Item-specific (from Product Data)

  • No reaction-condition data are provided for this item.

• If you are performing biochemical “reactions” (assays) with this compound (general guidance)

  • Enzymatic assays: Optimize buffer, temperature (often 25–37°C), cofactors (e.g., Mg2+, ATP), and incubation time according to the enzyme system. Include a DMSO vehicle control matching the highest compound concentration.
  • Solubility/stability: Confirm absence of precipitate at the working concentration and time. Assess stability by LC at assay temperature.

For preparative organic reactions, please select appropriate reagents/solvents from our synthesis catalog; this product is not intended for that purpose.

Safety and Handling

• Item-specific (from Product Data)

  • GHS Classification: Not specified for this item; refer to SDS.
  • Signal Word: Not specified for this item; refer to SDS.
  • Hazard Statements (H-Statements): Not specified for this item; refer to SDS.
  • Pictograms: Not specified for this item; refer to SDS.
  • Storage Conditions: Store at -20°C
  • Shipped In: Ice chest + Ice pads

• General laboratory safety (not a substitute for SDS)

  • PPE: Lab coat, safety glasses, and appropriate chemically resistant gloves (e.g., nitrile). Handle in a fume hood to avoid inhalation of dust or aerosols.
  • Incompatibilities: Avoid strong oxidizers and strong acids/bases until compatibility is known. Keep away from moisture if compound is moisture-sensitive; reseal promptly after opening.
  • First aid (general): If skin/eye contact occurs, rinse with water for ≥15 minutes and seek medical attention. If inhaled, move to fresh air. If ingested, rinse mouth; do not induce vomiting; obtain medical attention.
  • Spill response: Avoid dust generation. Collect with inert absorbent or damp disposable towels; dispose in accordance with institutional and local regulations.
  • Waste: Treat as organic laboratory waste (halogenated vs non-halogenated stream per solvent used). Deactivate bioassay test solutions by appropriate chemical means before disposal.

• Special risks (general for bioactive small molecules)

  • Unknown toxicology: Handle on a need-to-know, minimum-exposure basis. Use dedicated tools, weigh boats, and lined containers to prevent cross-contamination of shared equipment.
  • Light/oxygen sensitivity: Store in amber, tightly sealed vials under inert headspace when feasible.

Solvent Selection

• Item-specific (from Product Data)

  • Solubility/miscibility: Not specified for this item; refer to CoA/Spec Sheet.

• Practical solvent strategy for small-molecule probes (general guidance)

  • Primary solvent: Anhydrous DMSO is the default for initial dissolution due to broad solvating power for aromatic/heteroaromatic scaffolds and compatibility with biological assays at ≤0.1–1% v/v.
  • Secondary solvents: DMF and NMP offer high solvency but present higher cytotoxicity; use sparingly and dilute promptly into assay buffers. Ethanol or isopropanol can work for moderately lipophilic compounds when DMSO is undesirable.
  • Aqueous systems: Direct dissolution in water or PBS is usually poor for hydrophobic kinase inhibitors; use co-solvent strategies (DMSO or ethanol seed) and surfactants (e.g., 0.01–0.05% Tween-20) if biologically acceptable.

• Selection considerations

  • Polarity and dielectric: Choose higher-dielectric solvents (DMSO, DMF) for polar heterocycles; choose mid-polar protic (EtOH, i-PrOH) for moderately lipophilic compounds.
  • Assay compatibility: Keep final organic content low to avoid confounding biological readouts. Include vehicle controls matching the final solvent concentration.
  • Stability: Evaluate chemical stability by LC over time in your chosen solvent at storage and assay temperatures; some motifs undergo hydrolysis or oxidation in aqueous media.

• Quick comparison (general)

  • DMSO: Excellent solvency; good assay compatibility at ≤1%.
  • Ethanol: Lower solvency; higher allowable final % in some assays.
  • DMF/NMP: High solvency; limit exposure for cells/enzymes.

Storage and Reconstitution

• Item-specific (from Product Data)

  • Storage Conditions: Store at -20°C
  • Shipped In: Ice chest + Ice pads

• General storage guidance for small-molecule bioactives (context; not item-specific specifications)

  • Protect from light and moisture. Store in amber vials with PTFE-lined caps. If feasible, backfill with inert gas and include desiccant.
  • Once opened, promptly prepare single-use aliquots to minimize freeze–thaw and headspace oxygen exposure.

• Reconstitution (general guidance)

  • Solvent: Anhydrous DMSO is commonly used to prepare concentrated stocks (e.g., 10–50 mM). Actual solubility for this item is not specified; verify experimentally.
  • Procedure: Allow vial to warm to room temperature in a desiccator before opening to avoid condensation. Weigh quickly, add measured DMSO, vortex/sonicate until fully dissolved. If needed, gently warm (≤37°C) briefly.
  • Filtration: If particles persist, pass through a 0.2 µm PTFE syringe filter.
  • Aliquoting: Dispense single-use volumes into low-bind, amber microtubes. Store aliquots at -20°C to -80°C as appropriate.
  • Freeze–thaw: Avoid repeated cycles; thaw on ice or at room temperature just before use. Do not refreeze partially used aliquots.

• Stability checks

  • Periodically verify integrity of frozen stocks by LC or 1H NMR. Discard if degradation or precipitation is observed after thawing.

• Labeling

  • Record concentration, solvent, date of preparation, and operator initials on each aliquot. Maintain chain-of-custody documentation for regulated studies.

Structure and Identity

Brief description: Ro 32-7315 is listed as a small-molecule research compound in our small-molecule/compound library category.

• Item-specific (from Product Data)

  • SKU: R1441914
  • Product Name: Ro 32-7315
  • CAS: 219613-02-2
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

• Literature/general information (for context; not item certification)

  • Compound type: Small-molecule bioactive tool compound frequently cited as a kinase-pathway modulator (literature). Specific structure and stereochemistry vary by reported source; consult primary references for definitive identity.
  • Structural features (descriptive, literature): Many “Ro-” series protein kinase C (PKC) modulators share polyaromatic and heteroatom-containing pharmacophores enabling ATP-competitive or allosteric interactions (literature). Exact ring systems/functional groups for Ro 32-7315 should be verified from an authenticated reference or the item’s CoA.

• 2D structure (verbal description)

  • Not specified for this item; refer to CoA/Spec Sheet. If structural confirmation is required, we recommend acquiring NMR (1H/13C), HRMS, and, where available, comparison to literature spectra.

Notes: Because structural identifiers are not provided here, users should rely on the lot-specific CoA or independently confirm identity prior to critical experiments.

Synthetic Utility

• Item-specific (from Product Data)

  • This product is offered as a finished small-molecule probe. No functional handles, building-block intent, or derivatization pathways are specified.

• General remarks

  • Small-molecule kinase probes are not typically used as substrates or reagents in preparative organic synthesis due to their complexity and lack of orthogonally protected functional groups.
  • If analog synthesis is of interest, consult primary literature for Ro-series structure–activity relationships (SAR) to identify modifiable positions and known bioisosteres. Such work would involve custom synthesis rather than use of this catalog item as a reagent.

• Recommendations

  • For medicinal chemistry campaigns, request the CoA and, if available, limited structural information to design analytical comparators (LC, NMR) and to guide scaffold hopping.
  • For bioconjugation or immobilization studies, evaluate whether a linker-bearing analog is documented; direct modification of this item is not advised without confirmed structure and reactivity data.

Target Specificity

• Item-specific (from Product Data)

  • Target(s), isoform selectivity, Ki/IC50: Not specified for this item.
  • Species reactivity/biological panel: Not specified for this item.

• Literature/general context

  • Ro-series compounds are commonly reported as modulators of PKC family kinases; however, potency and selectivity vary by analog and assay format. Do not assume a profile for Ro 32-7315 without consulting primary literature or generating in-house data.

• Recommendations

  • Verify target engagement using orthogonal readouts (biochemical potency, cellular phospho-biomarkers, chemoproteomics, or CETSA/DSF).
  • Run a focused counterscreen against related kinases to quantify selectivity if your conclusions depend on target specificity.