(S)-V-0219 , CAS No.3031984-78-5

CAS: 3031984-78-5 Cat. No.: S1433193 Summenformel: C₂₀H₂₅F₃N₄O₂ Molekulargewicht: 410.43

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Übersicht

(S)-V-0219 is an enantiomer of V-0219. V-0219 is an orally active and positive allosteric modulator (PAM) of the GLP Receptor -1 ( GLP-1R ). (S)-V-0219 activates calcium fluxes in HEK cells stably expressing hGLP-1R. (S)-V-0219 is orally active and ameliorates high glucose levels in mice and inhibits feeding behavior in fasted mice.

Spezifikationen

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Aktionsart

MODULATOR

Namen und Kennungen

Molekulargewicht

410.43

Application Protocols

No vendor-validated application protocols are provided for this item.

General handling protocol for small-molecule screening stocks:

• Allow vial to equilibrate to room temperature before opening to avoid condensation ingress.
• Prepare a concentrated DMSO stock (e.g., 10–50 mM) after confirming solubility; record exact concentration by weight-in or quantitative NMR where applicable.
• Aliquot into low-bind, inert vials or plates to minimize freeze–thaw cycles; cap with PTFE-lined closures.
• Store working stocks at recommended temperature; protect from light if the compound contains chromophores (unknown here—err on the side of using amber vials).
• Before use, visually inspect for precipitation or discoloration; verify purity by LC–MS/HPLC at defined intervals during study.

Assay setup notes (generic):

• Titrate the compound across a suitable concentration range; include vehicle controls and, if available, a known positive control for benchmarking.
• Track final vehicle content and ensure compatibility with assay components.

Biological Roles

The specific biological roles, targets, or pathway interactions of (S)-V-0219 are not provided.

General considerations for small-molecule library members used in research:

• Potential roles include acting as enzyme modulators, receptor ligands, signaling pathway probes, or chemical biology tools; definitive function requires empirical data specific to this compound.
• If the compound is chiral, enantioselective interactions with biomacromolecules may lead to distinct potency/affinity profiles between enantiomers. Verify enantiomeric purity and consider testing the antipode as a selectivity control if available.
• For cellular studies, determine permeability (e.g., PAMPA, Caco-2), metabolic stability (microsomes, S9), and protein binding (plasma or assay media) to contextualize observed activity.
• Orthogonal target deconvolution approaches (thermal shift, CETSA; chemical proteomics; CRISPR knockouts) can be employed once an activity phenotype is observed.

No clinical or therapeutic claims are made. All uses are for research purposes only. Please consult assay data, SDS, and CoA for compound-specific guidance.

Buffer Applications

This product is not described as a buffering agent, and no acid/base properties are disclosed. Therefore, buffer formulation guidance is not typically applicable.

If you plan to dose (S)-V-0219 into buffered media for biological assays:

• Prepare concentrated DMSO stocks and dilute into the desired buffer (e.g., PBS, HEPES, Tris) keeping final DMSO ≤0.5–1% to reduce vehicle effects.
• Monitor for precipitation upon dilution; pre-warm solutions or add slowly with mixing. If necessary, include minimal co-solvent compatible with your assay.
• Verify that the buffer components do not chemically react with the compound (e.g., primary amines in Tris may interfere with activated ester chemistry if derivatization is performed in-buffer).

Green Alternatives

Because the chemical identity and solvent needs of (S)-V-0219 are not disclosed here, specific greener-substitute recommendations cannot be made for the compound itself. Nevertheless, the following general principles can improve the sustainability profile of handling small-molecule library members:

• Prefer greener solvents for workup and purification when compatible: ethyl acetate, methyl tert-butyl ether (MTBE), cyclopentyl methyl ether (CPME), and 2-methyltetrahydrofuran (2-MeTHF) over chlorinated solvents.
• For analytical HPLC, consider MeCN–water gradients; if substitutable, ethanol–water can be explored for certain analytes, acknowledging pressure/viscosity tradeoffs.
• Minimize DMSO waste by preparing concentrated master stocks and aliquoting; avoid repeated freeze–thaw to reduce discarded material.
• Adopt microscale screening where possible to cut solvent usage, and use 96/384-well formats with automated low-volume dispensing.

Illustrative tradeoffs (general):

• 2-MeTHF vs THF: 2-MeTHF offers bio-based origin and lower peroxide formation risk but has different polarity and miscibility; verify solubility and chromatographic behavior.
• EtOAc vs DCM: Ethyl acetate is less hazardous and more biodegradable; however, DCM may provide superior solvency for highly lipophilic analytes. Validate extraction efficiency before substitution.

Pharmaceutical Uses

No pharmacopeial status, excipient function, or formulation role is provided for (S)-V-0219. This product is supplied strictly for research use only.

General notes (non-clinical, research context):

• Compounds from screening libraries may be used as tool molecules in preclinical research, assay development, or mechanism studies. Any development toward dosage forms requires extensive characterization not covered by this listing (solid-state form, polymorphism, stability, impurities, residual solvents).
• If exploring preformulation in a research context, assess solubility in biorelevant media, pH solubility profile, and basic stability (pH 1–8, oxidative stress, light) to guide selection of enabling technologies (e.g., amorphous dispersions, lipid vehicles) purely for experimental purposes.

No medical, clinical, or therapeutic claims are made.

Physical Properties

Item-specific physicochemical properties are not provided in this record. Use the CoA/Spec Sheet for lot-specific values.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• logP/logD, pKa: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/general):

• Many chiral small molecules are shipped as solids or concentrated solutions. If solid, assess solubility in DMSO first for screening; if solution, check stabilizers and concentration.
• If the compound is moisture-sensitive or base/acid-labile, handle under dry inert atmosphere; perform a Karl Fischer or use known anhydrous solvents if sensitivity is suspected.
• For poorly soluble analytes, consider cosolvent systems (e.g., DMSO stock diluted into aqueous buffer with 0.5–2% final DMSO) or non-aqueous media (MeCN, MeOH) for analytical characterization.
• Measure UV–vis profile if you intend to use HPLC-UV quantitation; confirm no interfering absorbance from stabilizers if present.

Quality and Grades

Grade/purity are not specified in the current listing; consult the CoA/Spec Sheet for the supplied lot.

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
• Intended use: Research use only (as declared). Not for human or veterinary use.

Guidance on interpreting grades (general):

• Screening compound/library grade typically emphasizes identity and purity suitable for biochemical and cell-based assays (often ≥95% by HPLC/LC–MS), but acceptance criteria vary by supplier. Verify by CoA.
• Chiral compounds often include enantiomeric excess (ee) specifications and chiral HPLC traces. If not listed, request them when stereochemical fidelity is critical.
• Residual solvent and water content may impact solubility and bioassay performance. If not specified, consider independent KF and residual solvent GC headspace analysis.
• If UV transparency is important (e.g., HPLC method development), request a UV profile or ensure your detection wavelength avoids compound absorbance maxima.
• Documentation: Ensure CoA includes structure, CAS, analytical method, purity assay, and any stabilizer/additive. Keep lot traceability for screening datasets.

Reaction and Applications

No manufacturer application notes are provided for this item. Without a disclosed structure, reaction-specific guidance cannot be given.

General considerations for chiral small molecules:

• If used as a building block or reference standard, confirm stereochemical integrity by chiral chromatography and, if needed, optical rotation against literature or vendor data.
• For library screening, maintain a compound management workflow that mitigates freeze–thaw cycles (aliquoting) and limits ambient exposure to reduce degradation.
• If derivatization is intended (e.g., tagging for target engagement studies), select chemistries compatible with the presumed functional groups after structural confirmation (e.g., amide coupling for carboxylic acids/amines; click chemistry for azides/alkynes).
• When planning synthesis or late-stage diversification, assess potential liability motifs (e.g., anilides, benzyl ethers, esters) for hydrolysis/oxidation, and design stability-indicating LC methods.

Please consult the CoA/Spec Sheet for structural details to enable reaction planning (functional groups, protecting groups, and any salt/counterion information).

Reaction Conditions

No reaction condition guidance can be provided without the structure and functional groups of (S)-V-0219.

General practices (literature/general) when handling unknown or newly acquired chiral small molecules:

• Conduct a small stability screen: dissolve in intended solvent(s) and monitor by LC–MS/HPLC over 24–72 hours at room temperature and 37°C; test sensitivity to acid/base and air/light.
• If performing coupling chemistry, begin with mild conditions at low temperature and short reaction times; monitor for epimerization by chiral HPLC.
• Choose inert atmospheres for reactions prone to oxidation/hydrolysis; dry solvents and degas as appropriate.
• Typical analytical setup: reversed-phase HPLC (C18) with water–MeCN (0.1% formic acid or ammonium formate as needed) for purity and stability tracking.

For specific conditions, obtain the structural details from the CoA and consult the primary literature for the identified functional motifs.

Safety and Handling

GHS hazard information is not provided in this record. Always consult the product’s SDS for authoritative safety data and follow institutional EHS policies.

• Signal word / H-statements / Pictograms: Not specified for this item; refer to SDS.
• Primary risks: Unknown without SDS. Treat as potentially harmful if swallowed, inhaled, or in contact with skin/eyes. Avoid dust or aerosol formation.
• Personal protective equipment: Laboratory coat, safety glasses or goggles, and suitable chemical-resistant gloves (e.g., nitrile). Use a certified fume hood when weighing, dissolving, or transferring.
• Handling: Minimize exposure. Avoid incompatible materials until identified (oxidizers, strong acids/bases, reducing agents). Use tools dedicated to chiral compounds to avoid cross-contamination in stereochemical studies.
• First aid (general):
  • Inhalation: Move to fresh air; seek medical advice.
  • Skin contact: Wash with soap and water; remove contaminated clothing.
  • Eye contact: Rinse cautiously with water for several minutes; remove contacts if easy; seek medical attention.
  • Ingestion: Rinse mouth; do not induce vomiting unless directed; seek medical attention.
• Spill/accident: Contain solids with inert absorbent, avoid dust, collect in appropriate chemical waste.
• Fire-fighting: Use CO2, dry chemical, or foam; combustion products unknown—self-contained breathing apparatus recommended.

Solvent Selection

Item-specific solvent preferences are not provided. Selection should be guided by your application (analytical vs biological screening) and preliminary solubility tests.

General guidance for small-molecule library members:

• Stock preparation for screening: Start with DMSO at 10–50 mM; confirm solubility visually and by LC–MS/HPLC. Filter sterilize if needed for biological assays.
• Alternative organic solvents: Acetonitrile or methanol are common for LC methods; DMF or DMA may rescue solubility for polar/poorly soluble compounds but consider assay compatibility.
• Aqueous compatibility: When diluting into buffers, keep final DMSO ≤0.5–1% for sensitive cell assays; include nonionic surfactant (e.g., 0.01–0.05% polysorbate 20) if precipitation occurs, subject to assay tolerance.
• Inert handling: If the compound is oxidation- or hydrolysis-prone (unknown here), prepare solutions under nitrogen/argon in dry solvent and use amber vials.

Comparison (general):

• DMSO: Highest solvency; may affect biology at >1–2% v/v.
• MeCN: LC-friendly, volatile; lower solvency than DMSO.
• MeOH/EtOH: Good solvency for many heterocycles; potential assay interference.
• 2-MeTHF/EtOAc: Preferable for workups/extractions; less common for assay stocks.

Storage and Reconstitution

Item-specific storage and shipping information is provided; reconstitution details are not disclosed.

• Storage conditions: Store at -20°C (as provided in Product Data). Keep container tightly closed. Consider desiccation and inert atmosphere if moisture/oxygen sensitivity is suspected.
• Shipped in: Ice chest + ice pads (as provided in Product Data).
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet.

General best practices:

• If supplied as a solid: Equilibrate to room temperature in a desiccator before opening; quickly weigh and prepare concentrated stock (e.g., in DMSO) to minimize ambient exposure.
• If supplied as a solution: Verify concentration, solvent, and any stabilizers; store as indicated and avoid unnecessary freeze–thaw.
• Aliquoting: Prepare single-use aliquots to prevent degradation from repeated temperature cycling.
• Stability monitoring: Periodically check purity by LC–MS/HPLC; note any changes in color, precipitation, or retention time.
• Light and moisture: When in doubt, use amber vials and include desiccant. Maintain accurate inventory with lot numbers and receipt dates for traceability.

Structure and Identity

Brief overview: (S)-V-0219 is listed as a small-molecule library member. Item-specific structural identifiers are not disclosed in the current record.

• Product name: (S)-V-0219 (SKU: S1433193)
• CAS: 3031984-78-5
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChI / InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• Stereochemistry: The (S)- prefix indicates a single defined stereocenter with S-configuration; the exact stereocenter location is not disclosed in this record.

2D structure description (general): Without a disclosed structure, functional groups, ring systems, and substituent patterns cannot be summarized. For accurate structural interpretation, please consult the CoA/Spec Sheet or reach out to Aladdin Scientific technical support with the lot-specific documentation.

Identity verification suggestions (general guidance):

• Acquire vendor CoA containing structure, analytical method, and acceptance criteria.
• Confirm by orthogonal techniques where possible (e.g., HRMS for exact mass; 1H/13C NMR; chiral HPLC to verify enantiomeric configuration and purity; IR for functional-group checks).
• For screening libraries, consider LC–MS to establish retention time and purity relative to the supplied reference.

Synthetic Utility

The synthetic utility of (S)-V-0219 cannot be detailed without a disclosed structure. As a chiral small molecule, it may serve as a building block, reference standard, or screening hit, but such roles are speculative without functional-group information.

General strategy if using as a synthetic intermediate (after confirming structure):

• Map functional handles (e.g., amine, alcohol, carboxyl, halide) and plan protective-group strategy compatible with the chiral center to avoid racemization.
• For carboxylic acids or amino acids, use coupling conditions that preserve stereochemistry (e.g., EDCI/HOBt analogs or modern uronium salts with base control, low temperature). For secondary alcohols, Mitsunobu-like inversions or activation conditions should consider neighboring-group participation and epimerization risks.
• If late-stage diversification is desired, leverage C–H functionalization or cross-coupling on aryl/heteroaryl motifs, verifying the tolerance of the stereocenter.

Please consult the CoA/Spec Sheet for definitive structural information before planning transformations.

Target Specificity

No target, epitope, or selectivity information is provided for (S)-V-0219.

• Target(s): Not specified for this item; refer to CoA/Spec Sheet or screening data.
• Assay validation: Not provided.
• Species reactivity: Not applicable/Not provided.

If used as a chemical probe candidate, establish specificity empirically via:

• Counter-screens against close homologs or off-target panels.
• Orthogonal assay formats (biochemical vs cellular) to confirm on-target mechanism.
• Structure–activity relationship (SAR) using analogs or stereochemical controls where available.

Häufig gestellte Fragen

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 3031984-78-5, the molecular formula is C20H25F3N4O2, and the molecular weight is 410.43 g/mol.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.