S24–14 , CAS No.2348566-35-6

CAS: 2348566-35-6 Cat. No.: S1450781 Summenformel: C₁₉H₁₂N₂O₃S Molekulargewicht: 348.38

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Übersicht

S24–14 is a potent antiosteoporosis agent. S24–14 inhibits osteoclastogenesis with an IC 50 value of 0.40 µM. S24–14 induces osteoblast differentiation. S24–14 suppresses bone loss.

Specifications

Storage

Store at -20°C

Verschickt in

Ice chest + Ice pads

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Namen und Kennungen

• Molekulargewicht: 348.38

Application Protocols

Tested application data are not provided for S24–14 in this listing.

General protocol guidance (to be adapted once the product class is known):

• Small-molecule tool compound: Prepare a concentrated DMSO stock (e.g., 10–50 mM) if compatible; perform a dilution series; include vehicle controls; preincubate with target enzyme/cells as appropriate; readout by enzymatic rate, reporter signal, or viability.
• Peptide reagent: Reconstitute in suitable buffer; quantify by UV or weight; store aliquots at −20°C; avoid repeated freeze–thaw; use low-bind plastics to minimize adsorption.
• Antibody/affinity reagent (only if applicable): Follow manufacturer-recommended dilutions for WB/IHC/IF/FC; none are provided here, so titrate empirically.

Because no application details are supplied, users should validate conditions in pilot experiments and consult the CoA/SDS for any specific instructions.

Biological Roles

This listing does not specify a molecular identity, target, or mode of action for S24–14; therefore, definitive biological roles cannot be assigned.

General considerations for life science reagents (to be adapted once identity is known):

• Small-molecule tools: Often used to modulate enzyme or receptor function to dissect pathways; typical readouts include phosphorylation states, reporter assays, or transcriptional changes.
• Peptides: May function as substrates, inhibitors, mimetics, or antigens; roles depend on sequence, modifications, and conformation.
• Oligonucleotides: Can act in hybridization assays, gene regulation studies, or as qPCR standards.

Best practices:

• Confirm the reagent’s exact identity, salt form, and counter-ions to calculate accurate dosing (µM or mg/kg in model organisms—if permitted under your IACUC/ethics protocols; note this product is for research use only and not for clinical use).
• Validate pathway engagement with orthogonal assays (e.g., biochemical vs. cellular) to avoid off-target interpretations.

Note: For target-specific usage, consult the lot’s CoA, any associated technical bulletins, or peer-reviewed articles referencing “S24–14” with the same CAS (2348566-35-6) to ensure you are matching the reagent identity.

Buffer Applications

No buffer system or reconstitution buffer is specified for S24–14 in this listing.

Guidance once identity is known:

• Small molecules: Prepare concentrated stocks (e.g., 10–50 mM) in DMSO, then dilute into assay buffer (PBS, HEPES, Tris) while keeping final DMSO ≤0.5–1% to minimize biological effects.
• Peptides/proteins: Choose buffer based on isoelectric point and stability—common options are PBS (pH 7.2–7.4), HEPES (pH 7.2–7.6), or ammonium bicarbonate for MS workflows. Additives such as 0.1% BSA or 0.05% Tween-20 can reduce adsorption, if compatible.
• Oligonucleotides: Nuclease-free water or TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) is typical.

Practical tips (general):

• Filter buffers (0.22 µm) and degas if required by the assay (e.g., ITC, SPR).
• Avoid metal chelators if your assay requires metal-dependent enzymes; conversely, include EDTA if nuclease inhibition is needed.

Because the compound class is unknown here, select buffers only after consulting the lot-specific CoA/Spec Sheet and aligning with your assay’s requirements.

Green Alternatives

Because the identity and solvent recommendations for S24–14 are not provided, a tailored green-alternatives analysis is not possible for this item.

General guidance (apply once class/solvent is known):

• If DMSO is the primary stock solvent, consider alternatives like glycerol, PEG 400, or aqueous buffers when compatible with solubility and biological assays to reduce VOC use.
• When chromatographic purifications are required (for method development or QC), explore greener eluents (ethyl acetate/ethyl lactate/isopropanol in place of dichloromethane or hexanes where feasible).
• For routine rinses and cleaning steps, replace chlorinated solvents with alcohols or water-based detergents when compatible.
• Scale considerations: minimize stock concentrations and prepare just-in-time aliquots to reduce waste and repeated freeze–thaw cycles.

Trade-offs (general):

• Greener solvents may alter solubility, assay background, or permeability. Validate bioactivity and stability in the alternative medium before adoption.

Recommendation: Once the chemical class and use-case are confirmed via CoA or internal documentation, perform a brief solvent and waste assessment to identify realistic greener substitutions without compromising data quality.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or formulation details are provided for S24–14 in this listing. This product is supplied strictly for research use only and is not intended for human or veterinary use, diagnostic procedures, or as an API.

General considerations for formulation scientists (if applicable to identity):

• Preformulation: Determine solubility across pH, pKa, polymorph/salt form, and stability (oxidative, hydrolytic, photolytic). For peptides/biologics, assess aggregation propensity and storage buffer compatibility.
• Excipients: Select biocompatible carriers (e.g., cyclodextrins for poorly soluble small molecules; stabilizing sugars or amino acids for peptides/proteins) only if aligned with your non-clinical research objectives.
• Delivery for in vivo research: Follow institutional approvals. Record vehicle composition and osmolality; filter-sterilize when necessary.

Note: In the absence of grade/purity and identity, no claims can be made regarding compliance with USP/EP/JP or ICH guidelines for clinical development.

Physical Properties

Item-specific properties (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Commonly requested specs (this listing):

• Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (BP): Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• pKa / logP (or logD): Not specified for this item; refer to CoA/Spec Sheet.
• UV/Vis characteristics (λmax, cutoff): Not specified for this item; refer to CoA/Spec Sheet.
• Solubility profile: Not specified for this item; refer to CoA/Spec Sheet.

Practical guidance (general/literature-style; select as applicable once identity is known):

• Small organic molecules are often reconstituted in dry DMSO, DMF, ethanol, or water/buffer depending on ionization state; check CoA for recommended solvent and target stock concentrations.
• Peptides typically dissolve in water, dilute acetic acid, basic aqueous buffer, or DMSO depending on net charge and hydrophobicity (consult sequence and pI).
• Oligonucleotides are generally supplied lyophilized and are reconstituted in nuclease-free water or TE buffer; concentration verified by A260.
• Hygroscopic materials should be handled rapidly under dry atmosphere; photosensitive materials stored ambered.

Quality and Grades

Item-specific grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret grades (general guidance):

• Research grade indicates suitability for laboratory R&D but is not certified for clinical or diagnostic applications. Purity is typically established by HPLC/UPLC and/or NMR/HRMS.
• If the item is a small-molecule modulator, “≥95% (HPLC)” or similar may be reported on the CoA; peptides often list purity as % area by RP-HPLC.
• Residual solvent levels, water content (Karl Fischer), counter-ion content (for salts), and inorganic impurities may be provided in the Spec Sheet; these define fitness for sensitive assays (biochemical or cell-based).
• UV-Vis background and fluorescence impurity profiles can matter for optical readouts; HPLC- or spectrophotometry-grade solvents and materials are recommended for such assays.

What to check on receipt:

• Match lot number and CAS with your purchasing record.
• Review analytical data: chromatogram, MS, NMR (if available), peptide sequence verification, endotoxin/bioburden (if applicable).
• Confirm stabilizers, salt form, and water of hydration if relevant to accurate dosing.

Note: All values not present in this catalog entry must be taken from the lot-specific CoA/Spec Sheet to avoid over-interpretation.

Reaction and Applications

Manufacturer Applications (from catalog): Not specified for this item.

Given the Life Science categorization and lack of disclosed structure, synthetic reaction applications cannot be assigned confidently. If S24–14 is a biochemical tool compound (e.g., enzyme modulator, probe, or peptide), applications would center on in vitro or cell-based assays rather than bench-scale chemical transformations.

General possibilities (context-dependent; verify via CoA/SDS or primary literature for this specific code):

• Small-molecule modulators: target engagement in biochemical assays, selectivity profiling, thermal shift assays (DSF), and cell signaling readouts.
• Peptides/biologics: binding studies, pull-downs, ELISA standards, or as positive controls in blotting/IF if tagged.

Tips for reproducibility:

• Establish a validated dilution series (e.g., 10-point 1:3) in an assay-compatible vehicle with appropriate negative/vehicle controls.
• Confirm identity and purity by independent analytics (HPLC/HRMS; peptide mass mapping) before critical studies.
• Track freeze–thaw cycles and time-in-solution to mitigate degradation.

Note: Do not infer specific reaction chemistry (e.g., cross-coupling, Grignard) without structural data; this section is intentionally generic due to missing item details.

Reaction Conditions

No reaction conditions are specified for S24–14 in this listing.

General guidance (conditional on identity):

• Small molecules: Optimize solvent (DMSO, MeCN, MeOH, toluene), temperature (0–120°C as needed), and catalysts/bases/acids according to functional groups. Monitor by TLC, LC-MS, or UPLC. Typical scales prioritize micro- to millimole for tool compounds.
• Peptides: If modifications are intended, use mild conditions compatible with amide backbones (e.g., carbodiimide couplings, orthogonal protecting-group strategies). For labeling, NHS-esters (pH 8.3) or maleimides (pH 6.5–7.0) target Lys/Cys respectively.
• Bioconjugations: Perform under aqueous conditions with controlled stoichiometry and pH; confirm by MS and SDS-PAGE (for proteins).

Always verify solvent compatibility and stability against hydrolysis/oxidation before attempting any transformations. In the absence of structural data, do not assume standard conditions apply.

Safety and Handling

GHS classification (item-specific):

• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (always defer to the SDS and institutional EHS policies):

• Wear appropriate PPE: lab coat, safety glasses or face shield, and chemical-resistant gloves suitable for the solvent system used.
• Handle in a fume hood if there is potential for volatile, dust, or aerosol exposure. Avoid inhalation, ingestion, and skin/eye contact.
• Storage incompatibilities should be assessed once the chemical class is known (e.g., strong oxidizers, acids/bases, reactive metals). In the absence of details, store segregated in a closed, labeled container at the specified temperature.
• Spills: Contain and absorb with inert material. For powders, avoid creating dust; gently cover and collect. Dispose according to local regulations.
• First aid (overview): Rinse eyes/skin with water for at least 15 minutes; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth; do not induce vomiting unless directed. Seek medical attention in all exposure cases.
• Special risks: If the reagent is an organic peroxide former, azide, or other energetic class, specialized handling applies—verify via SDS. For peptides/biologics, avoid repeated exposure to minimize sensitization risk.
• Documentation: Obtain and review the lot-specific SDS and CoA prior to use; these contain authoritative hazards, handling, and disposal information.

Solvent Selection

Item-specific solvent recommendations are not provided in this listing; consult the CoA/Spec Sheet for definitive guidance.

General selection framework (use once identity/class is known):

• Small organic molecule (neutral or weakly polar): Start with anhydrous DMSO for stock solutions (10–50 mM typical), then dilute into assay buffer with ≤1% DMSO final. Alternatives: ethanol, DMF, or acetonitrile depending on solubility and assay tolerance.
• Ionic/ionizable compounds: Consider water, PBS, or buffers adjusted to favor the ionized state; use minimal organic cosolvent. For bases, 0.1 N HCl or acetate buffer; for acids, 0.1 N NaOH or Tris/borate (small volumes) can aid dissolution.
• Peptides: Begin with water; if insoluble, try dilute acetic acid (0.1–1%), basic buffers (e.g., 10–50 mM NH4HCO3), or DMSO for highly hydrophobic sequences. Sonication and gentle warming can help.
• Oligonucleotides: Use nuclease-free water or TE buffer; avoid organic solvents.

Practical notes:

• Filter stock solutions (0.22 µm) if assay-compatible and if the compound is not adsorptive/unstable.
• Record solvent lot and water content; anhydrous conditions may be critical for moisture-sensitive reagents.
• Verify concentration by weight/volume and, where possible, by UV or quantitative NMR.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Store at −20°C (per Product Data).
• Shipped In: Ice chest + ice pads (per Product Data).

Additional item-specific details (appearance, stabilizers, recommended solvent, concentration): Not specified for this item; refer to CoA/Spec Sheet.

General reconstitution guidance (adapt to identity once known):

• Small molecules: If solid, allow vial to equilibrate to room temperature in a desiccator before opening to avoid condensation. Prepare a concentrated stock in a suitable solvent (commonly anhydrous DMSO) and aliquot into low-adsorption tubes. Record exact concentration by weight and, if feasible, by quantitative NMR or UV.
• Peptides/biologics: Centrifuge vial briefly to collect material. Start with sterile water; adjust pH with minimal acid/base if necessary or use appropriate buffer. For hydrophobic peptides, consider adding 10–50% acetonitrile or DMSO, then dilute with buffer. Filter-sterilize (0.22 µm) if compatible with downstream use.
• Oligonucleotides: Reconstitute in nuclease-free water or TE buffer; quantify by A260 and store in aliquots at −20°C.

Stability and handling:

• Protect from repeated freeze–thaw cycles; prepare single-use aliquots.
• Protect from light if chromophores are present (unknown here—use amber storage as a precaution).
• Observe any lot-specific expiry or retest date on the CoA; discard if precipitation, discoloration, or unexpected odor appears upon thawing (unless expected by solubility).

Structure and Identity

Short description: Item S24–14 is listed under Life Science reagents. The structural identity and composition are not disclosed in this catalog entry.

• Item-specific identifiers
  • SKU: S1450781
  • Product Name: S24–14
  • CAS: 2348566-35-6
  • Category Path: 全部 / 可售 / 生命科学
  • Research Use Note: For research use only
• Structure-related data (item-specific)
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general guidance)
  • Because no structural string or formula is provided, specific functional groups, ring systems, or stereochemical elements cannot be detailed for this listing.
  • If you have a lot-specific CoA or an internal project code for S24–14, align that with analytical confirmation (e.g., 1H/13C NMR, HRMS, HPLC/UPLC purity, and, where applicable, peptide sequence or oligonucleotide composition) before experimental use.
• 2D/functional description (general)
  • Without a structure, the compound class (small molecule, peptide, nucleic acid, etc.) cannot be inferred from the catalog entry. Please consult the lot’s CoA, SDS, or project documentation to determine the exact identity and handle accordingly.

Synthetic Utility

Synthetic utility cannot be detailed because the structure and functional groups of S24–14 are not provided in this catalog entry.

General remarks (choose based on identity once known):

• If S24–14 is a small organic intermediate: Evaluate handles for diversification (e.g., aryl halides for cross-coupling, carbonyls for condensations/reductions, amines for acylation/alkylation). Confirm protecting-group pattern and chemoselectivity.
• If a peptide: Consider it as a standard or building block for SAR studies—fragment coupling, N-to-C extensions, or side-chain modifications, guided by sequence and protecting groups.
• If an oligonucleotide: It may serve as a template or control; chemical modification (e.g., phosphorothioate, 2′-OMe) would define downstream reactivity and stability.

Recommendations:

• Obtain structural data (NMR, MS, elemental analysis, sequence confirmation) from the CoA/Spec Sheet before planning any transformations.
• Map stability domains (acid/base, redox, heat, light) to prevent decomposition during synthetic steps.

Target Specificity

Target, pathway, and specificity data are not provided for S24–14. No antigen, epitope, clone, or species reactivity information is listed.

• If this product is a biochemical tool: Consult the CoA/Spec Sheet or associated technical notes for the intended molecular target and any reported selectivity panel results.
• If this product is an antibody or affinity reagent: Target name, isotype, clonality, and cross-reactivity must be verified from product-specific documentation; none are supplied in this entry.

Until the identity is confirmed, no claims about specificity or selectivity should be made.