Synthetic Blocking Buffer, Blotting - Ready-to-use (RTU)

Cat. No.: S494457

GRADE & PURITY Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Übersicht

Product Characteristics 

The Synthetic Blocking Buffer, Blotting is a readyto-use solution for blocking of the remaining free binding sites after coating or transfer of biomolecules to a membrane. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal. 

The Synthetic Blocking Buffer, Blotting eliminates the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA

Composition & Properties 

The Synthetic Blocking Buffer, Blotting is a readyto-use buffer. The buffer contains no proteins, and cross reactivity is therefore avoided.

Working Procedure 

1.Follow the normal procedure for transfer of the biomolecules to the membrane. 

2.Dry the membrane. 

3.Block the membrane by incubation in the Synthetic Blocking Buffer, Blotting for 5-10 minutes at room temperature and gentle shaking. 

4.Wash the membrane in a standard washing buffer for blotting. 

5. Continue the processing as usual.

Tips & Tricks 

• In ELISA, the Synthetic Blocking Buffer, ELISA (cat. no. S494401) is recommended.

Handling & Storage 

• Store solution at 2-8 °C.

Specifications

Spezifikationen & Reinheit

Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Verschickt in

Wet ice

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Chemische und physikalische Eigenschaften

• Empfindlichkeit: Light sensitive

Häufig gestellte Fragen

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.