10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 4 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
TAME Tosyl-L-Arginine Methyl Ester (TAME) is an APC inhibitor.
In vitro
TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 µM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. TAME is hydrolysed by trypsin with Km of 0.328 mM. TAME accelerates the ATP hydrolysis process about 12-fold. TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M.
In vivo
Cell Data
cell lines：HUVEC cells
Concentrations：
Incubation Time：
Powder Purity:≥99%
| Isomere SMILES | CC1=CC=C(C=C1)S(=O)(=O)N[C@@H](CCCN=C(N)N)C(=O)OC |
|---|---|
| Molekulargewicht | 342.41 |
| Reaxy-Rn | 26018177 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=26018177&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
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| Lot Number | Certificate Type | Datum | Artikel |
|---|---|---|---|
 A2303673 | Certificate of Analysis | Sep 18, 2026 | T409236 |
| 1. Yiyang Chen, Hao Ren, Xiangyu Kong, Hao Wu, Zhenmei Lu. (2023) A multicomponent propane monooxygenase catalyzes the initial degradation of methyl tert-butyl ether in Mycobacterium vaccae JOB5. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, [PMID:37823642] [10.1128/aem.01187-23] |
| 2. Yejun Zhong, Lei Yang, Ziyi Zhu, Hongyu Chen, Chengmei Liu, Taotao Dai, Er Sheng Gong. (2022) Protective effect of ovalbumin-flavonoid hydrogel on thrombolytic activity and stability of nattokinase. FOOD RESEARCH INTERNATIONAL, [PMID:35651046] [10.1016/j.foodres.2022.111188] |
| 3. Liu Ting, Chen Xiaopeng, Xing Jinfeng, Ge Zhiqiang. (2018) Effects of acetazolamide on the conformations and activities of digestive enzymes: pepsin and trypsin. MEDICINAL CHEMISTRY RESEARCH, 27 (5): (1549-1557). [PMID:] [10.1007/s00044-018-2172-2] |
| 4. Xiong Zheng, Ying Zhang, Maogang He. (2015) Speed of sound measurement in ethyl tert-butyl ether and tert-amyl methyl ether by Brillouin light scattering. FLUID PHASE EQUILIBRIA, [PMID:] [10.1016/j.fluid.2015.10.026] |
