Total Sugar Content Assay Kit (DNS, Micro Method)

Cat. No.: T1527579

QUALITÄT & REINHEIT BioReagent, Standard Curve Method

Übersicht

Carbohydrates are one of the important components of animal and plant tissues, and serve as both primary metabolites and storage substances. Total sugar mainly includes reducing sugars such as glucose, fructose, and lactose; disaccharides such as sucrose and maltose, which can be hydrolyzed to reducing monosaccharides under the assay conditions; and starches that may be partially hydrolyzed. This product is suitable for the determination of total sugar content in animal and plant tissues, as well as in liquid samples such as serum and plasma. The assay principle is as follows: Total sugars are first hydrolyzed to reducing sugars. The reducing sugars are then heated with DNS reagent under alkaline conditions, reducing DNS to an amino compound, which appears reddish‑brown in alkaline solution and exhibits a characteristic absorption peak at 540 nm. Within a certain concentration range, the reducing sugar content is linearly correlated with the absorbance at 540 nm. The total sugar content in the sample can be calculated using a standard curve.

Spezifikationen

Synonyme

Total Carbohydrate Content Assay Kit

Spezifikationen & Reinheit

BioReagent, Standard Curve Method

Note

BioReagent, Standard Curve Method

Anwendung

Carbohydrate Metabolism

Probentyp

Pflanzengewebe, Plasma, Serum, Tierische Gewebe

Erfassungsbereich

0.1-2 mg/mL

Methode des Nachweises

Spectrophotometry

Detection instrument

Microplate reader

Detection wavelength

540nm

Empfindlichkeit

Light sensitive

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ long term (12 months).

Contents & Storage

Component No.	Component Name	Appearance	48T	96T	Storage
T1527579A	Hydrochloric Acid	Liqiud	60mL	120mL	2-8℃.
T1527579B	Sodium Hydroxide	Liqiud	60mL	120mL	2-8℃.
T1527579C	DNS Reagent	Liqiud	2.4mL	4.2mL	2-8℃.Store at dark.
T1527579D	Standard	Solid	1 vial	1 vial	2-8℃.