Trypsase Activity Assay Kit (TAME, Micro Method)

Cat. No.: T1515849

QUALITÄT & REINHEIT BioReagent

Übersicht

Trypsin selectively hydrolyzes peptide bonds formed by the carboxyl groups of lysine or arginine in denatured proteins, making it an important digestive enzyme. Additionally, trypsin is widely used as an adjunct therapy for conditions such as local edema, hematomas, and abscesses resulting from empyema, hemothorax, surgical inflammation, ulcers, traumatic injuries, and similar cases. In this assay kit, trypsin catalyzes the hydrolysis of the ester bond in TAME, releasing free carboxyl groups. These groups undergo a neutralization reaction with sodium hydroxide present in the reaction system, leading to a decrease in the pH of the solution. Using phenol red as an indicator, the change in absorbance at 555 nm is measured, enabling rapid determination of trypsin activity. Within a certain range, trypsin activity exhibits a well-defined linear relationship with the decrease in absorbance at 555 nm.

Spezifikationen

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Anwendung

Enzyme activity assay

Probentyp

Pflanzengewebe, Tierische Gewebe

Methode des Nachweises

Colorimetric Method

Detection instrument

Microplate reader,Visible Spectrophotometer

Detection wavelength

555nm

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light

Verschickt in

Wet ice

Stabilität und Lagerung

Store at 2-8℃ for 6 months. Store in the dark.

Häufig gestellte Fragen

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.