Universal Nuclease, CAS No.9025-65-4

CAS: 9025-65-4 Cat. No.: B1523051 EG-Nummer: 964-102-5

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, Pharmaceutical grade, EnzymoPure™, ≥99%(SDS-PAGE), expressed in E.coli;≥250 U/μL; Protein concentration: See COA

Accession #

P13717

Expression system

E. coli

Bioactivity

≥250 U/μL

Übersicht

Universal Nuclease is a highly purified recombinant nuclease expressed in Escherichia coli. It can degrade all types of DNA and RNA, including single-stranded, double-stranded, linear and circular forms. Experimental results show that it exhibits excellent degradation activity against sperm DNA, viral nucleic acids and eukaryotic genomic DNA. The end products of complete digestion are oligonucleotide fragments of 2~5 bases, which are below the hybridization detection limit.

This product remains active at pH 6.0~10.0 and 0~42 ℃ in the presence of 1-2 mM Mg ²⁺ , and its activity is not affected by surfactants or reducing agents.

Main Applications:

1.Reduce solution viscosity during protein extraction;

2.Sample preparation for two-dimensional gel electrophoresis;

3.Removal of residual nucleic acids in biological products.

Activity Unit

One unit (U) is defined as the amount of enzyme required to increase the A260 absorbance of DNA by 1.0 when incubated at 37 ℃ for 30 min, equivalent to complete digestion of 37 μg DNA.

Recommended Digestion Conditions

Universal Nuclease	2000  U/L
pH	8.0-8.5
Temperature(℃)	37 ℃
Reaction Time(h)	1-2 h

Reagent Tolerance

Reagent	Experimental Result	Reagent	Experimental Result
DTT	≤500 mM, no effect on enzyme activity	PO 4 3-	≤100 mM, no effect on enzyme activity
BME	≤500 mM, no effect on enzyme activity	Mg 2+	Essential for enzyme activity; 100 mM does not inhibit enzyme activity
Na +	≤1 M, no effect on enzyme activity	Mn 2+	≤15 mM, no effect on enzyme activity
K +	≤500 mM, no effect on enzyme activity	Ca 2+	≤25 mM, no effect on enzyme activity
EDTA	≥0.1 mM, inhibits enzyme activity	Zn 2+	≤10 mM, no effect on enzyme activity
Triton X-100	≤5%, no effect on enzyme activity	Tween-80	≤5%, no effect on enzyme activity
SDS	≥0.1%, affects enzyme activity	(NH 4 ) 2+ SO 4	≤100 mM, no effect on enzyme activity
Urea	≤2 M, no effect on enzyme activity	GuHCl	≤0.5 M, no effect on enzyme activity
β-Propiolactone	≤0.05%, no effect on enzyme activity	Formalin	≤0.05%, no effect on enzyme activity

MS Analysis of Enzymatic Hydrolysis Products of Nucleic Acids 

MS analysis was performed on the products of plasmid DNA hydrolyzed by nuclease for 1 hour. The main hydrolysis products were 2-base and 6-base fragments, while other low-abundance fragments ranged from 3 to 20 bases. 

Specifications

Product Name

Universal Nuclease, CAS No.9025-65-4

Synonyme

Serratia marcescens nuclease

Note

ActiBioPure™, Bioactive, Pharmazeutische Qualität, Recombinant, EnzymoPure™

Spezifikationen & Reinheit

Bioactive, Recombinant, ActiBioPure™, EnzymoPure™, pharmaceutical grade, ≥99%(SDS-PAGE), expressed in E.coli;≥250 U/μL; Protein concentration: See COA

Biochemische und physiologische Mechanismen

Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.

Bioaktivität

≥250 U/μL

Beitritt #

P13717

CAS

9025-65-4

Enzym Kommissionsnummer

EC 3.1.30.2

Molekültyp

Enzyme

Lagerung und Versand

Konzentration

expressed in E.coli;≥250 U/μL; Protein concentration: See COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at -20℃ long term (36 months). Upon receipt, it is recommended to aliquot. Avoid storage under -70°C.

Definition der Einheit

One unit of activity is defined as the amount of enzyme required to increase the absorbance of DNA at A260 by 1.0 when incubated at 37°C for 30 minutes (equivalent to the amount of enzyme that completely digests 37 μg of DNA).

Referenzen

1. Yu Li, Miaomiao Cai, Wenwen Zhang, Ying Liu, Xiaoqing Yuan, Na Han, Jing Li, Shengnan Jin, Chunming Ding.  (2023)  Cas12a-based direct visualization of nanoparticle-stabilized fluorescence signal for multiplex detection of DNA methylation biomarkers.  BIOSENSORS & BIOELECTRONICS,      [PMID:37924654] [10.1016/j.bios.2023.115810]

2. Ruopeng Yin, Tao Wang, Jingzu Sun, Huanqin Dai, Yuting Zhang, Ningning Liu, Hongwei Liu.  (2024)  Postbiotics From Lactobacillus Johnsonii Activates Gut Innate Immunity to Mitigate Alcohol-Associated Liver Disease.  Advanced Science,      [PMID:39574408] [10.1002/advs.202405781]