Urease (URH), CAS No.9002-13-5

CAS: 9002-13-5 Cat. No.: U1492281 EC Number: 232-656-0

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, >150 U/mg protein; expressed in E.coli

Accession #

Q03282,Q03283,Q03284

Expression system

E. coli

Bioactivity

>150 U/mg protein

Overview

Ureases, functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Reaction Equation

Specification
Specific Activity: >150 U/mg protein
Contaminants:
NADPH oxidase: <0.001%
Properties
pH stability: pH 8.0-9.5 (37°C, 1 weeks)
Thermal stability: ≤ 65°C (pH 8.0, 10 min.)
Optimum pH: 6.0
Optimum temp.: ≥37°C
Km value: 1.94× 10⁻⁵ mmol/L (Urea)
Molecular weight: 60.3 kDa  α subunit, 11.7 kDa β subunit, 11.1 kDa λ subunit (SDS-PAGE)
Assay Procedure
I Spectrophotometric Method
Wavelength: 340 nm, Light path length: 1 cm
Temperature: 25°C
Pipette the following reagents into a cuvette:
3.00 mL: Triethanolamine-HCl buffer (0.1 mol/L, pH 7.0) containing Urea (1 mol/L)、α-Ketoglutarate (5 mmol/L)、NADPH (0.24 mmol/L)、GIDH (20 U/mL)
0.02 mL: rUrease (approx. 1.5 U/mL)
II Calculation

ΔA/min: The change in absorbance at 340 nm per minute
V: Total volume of reaction mixture (3.02 mL)
D: Enzyme dilution factor
6.2: mmol/L extinction coefficient of NADPH (L⋅mmol −1⋅cm −1)
d: Light path length (1 cm)
v: Volume of enzyme sample (0.02 mL)
Reference Data

Specifications

Product Name

Urease (URH), CAS No.9002-13-5

Synonyms

ureA | ureB | ureC

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

EnzymoPure™, ActiBioPure™, Recombinant, Bioactive, High Performance, >150 U/mg protein; expressed in E.coli

Bioactivity

>150 U/mg protein

Accession #

Q03282, Q03283, Q03284

Predicted molecular weight

60.3 kDa α subunit, 11.7 kDa β subunit, 11.1 kDa λ subunit (SDS-PAGE)

CAS

9002-13-5

Enzyme Commission Number

EC 3.5.1.5

Molecule Type

Enzyme

Storage and Shipping

Concentration

>150 U/mg protein; expressed in E.coli

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term.Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

References

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