V5 tag Mouse mAb - Primärer Antikörper, specific to V5, Mouse IgG2a

Cat. No.: Ab133823

GRADE & PURITY Carrier Free, ExactAb™, Validated, High Performance, 1.0 mg/mL

Application

IP, WB

Host species

Maus

Reactive species

Artenunabhängig

Isotype

Mouse IgG2a

Clone Number

K05002_3F6

Conjugation

Unconjugated

Übersicht

Mouse anti V5 tag Antibody, Monoclonal (K05002_3F6), could be used for WB, IP and so on.
Application:
WB: 1/3000-1/4000
IP: 1/100

Specifications

Product Name

V5 tag Mouse mAb - Primärer Antikörper, specific to V5, Mouse IgG2a

Synonyme

GKPIPNPLLGLDST tag antibody | Protein Rev antibody | Regulator of expression of viral proteins antibody | rev antibody | V5 epitope tag antibody | GKPIPNPLLGLDST Epitope Tag | Parainfluenza virus 5 V/P | Paramyxovirus SV5 | GKPIPNPLLGLDST epitope tag anti

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Validated, High performance, 1.0 mg/mL

Wirtsspezies

Maus

Spezifität

V5

Immunogen

A fusion protein containing V5.

Positive Kontrolle

WB: HEK293 cells transfected with T7 tag. IP: HEK-293 cell lysates transfected with V5 tagged CDA at C-terminus; HEK-293 cell lysates transfected with V5 tagged CDA at C-terminus and KT82 was used as IgG isotype control antibody; HEK-293 cell lysates tran

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, High Performance, Validated

Isotyp

Mouse IgG2a

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: V5 tag Mouse mAb (Ab133823)

V5 tag Mouse mAb (Ab133823) - Western Blot
All lanes: V5 tag Mouse mAb (Ab133823) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 50, 112 kDa
Exposure time: 1.16 s 

IP: V5 tag Mouse mAb (Ab133823)

V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged CDA at C-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag antibody at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: HEK293 lysate (transfected with V5 tagged CDA at C-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane1. Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.

IP: V5 tag Mouse mAb (Ab133823)

V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with 200 µg of lysate extracted from HEK293 transfected with V5 tagged ARF3 at N-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag protein at 1.0 µg/mL was used as the V5 tag Mouse mAb (Ab133823) and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: HEK-293 lysate (transfected with V5 tagged ARF3 at N-terminus).Lane 2: V5 tagged protein immunoprecipitated from the same lysate as Lane 1.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: V5 tag Mouse mAb (Ab133823) can immunoprecipitate V5 tag.

IP: V5 tag Mouse mAb (Ab133823)

V5 tag Mouse mAb (Ab133823) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of V5 tag Mouse mAb (Ab133823) with untransfected HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag Mouse mAb (Ab133823) at 1.0 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.Lane 1: Untransfected HEK293 lysate.Lane 2: Sample immunoprecipitated from untransfected HEK-293 lysate by KT25.Lane 3: IgG isotype control antibody instead of V5 tag Mouse mAb (Ab133823) in HEK293 lysate.Result: Unspecific immunoprecipitation by V5 tag Mouse mAb (Ab133823) was not obsearved.

Anwendung

Anwendung

Dilution info

IP

1/100

WB

1/3000-1/4000

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (V5 tag Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.