VII-31 - 10mM in DMSO , CAS No.2305757-96-2

CAS: 2305757-96-2 Cat. No.: V656322 Summenformel: C₂₃H₂₅NO₅S Molekulargewicht: 427.51 PubChem CID: 146673076

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Übersicht

VII-31 is a potent NEDDylation pathway activator to inhibit the tumor progression in vitro and in vivo. VII-31 induces apoptosis via intrinsic and extrinsic pathways

In Vitro

VII-31 (100 nM, 200 nM; 48 hours) inhibits the cell viability of gastric cell line MGC803 with an IC 50 of 0.09±0.01 μM. VII-31 also inhibits the cell viability of MCF-7 and PC-3 with IC 50 s of 0.10±0.006 and 1.15±0.28μM , respectively. VII-31 (50-150 nM; 24 hours) arrests MGC803 cells cycle in G2/M phase. VII-31 (50-150 nM; 48 hours) induces apoptosis via intrinsic and extrinsic pathways. VII-31 (50-150 nM; 24 hours) activates NEDDylation in MGC803 cells. VII-31 (50-150 nM; 48 hours) up-regulates pro-apoptotic proteins FADD, Fasl, PIDD, Bax, Bad; while down-regulates anti-apoptotic proteins Bcl-xL, Bcl-2, XIAP, c-IAP1. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Gastric cancer MGC803 cells Concentration: 100, 200 nM Incubation Time: 48 hours Result: Inhibited the cell viability in dose-depend manner. Cell Cycle AnalysisCell Line: MGC803 cells Concentration: 50, 100, 150 nM Incubation Time: 24 hours Result: Arrested cells in G2/M phase, and a clear sub-G1 peak was observed in the high dose group. Apoptosis AnalysisCell Line: MGC803 cells Concentration: 50, 75, 100, and 150 nM Incubation Time: 48 hours Result: High dose (150 nM) treatment significantly elevated the early and late apoptosis rate to 92.8% from 4.8%. Western Blot AnalysisCell Line: MGC803 cells Concentration: 50, 100, 150 nM Incubation Time: 24 hours Result: Resulted in NEDDylation activation of MGC803 cells, the NEDDylation of 3 important proteins NAE1, Ubc12 and CUL1 has been activated.

In Vivo

VII-31 inhibits the tumor progression in vivo, while showing no obvious toxicity to mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Mice bearing MGC803 xenograft tumors Dosage: 50, 100, 150 mg/kg Administration: Subcutaneous injection; daily for 28 days Result: The mice had a much smaller tumor compared with vehicle control. The tumor volumes of middle/high dose treated mice at certain time points were evidently decreased comparing with untreated group.

IC50& Target:NEDDylation

Specifications

Spezifikationen & Reinheit

10mM in DMSO

Biochemische und physiologische Mechanismen

VII-31 is a potent NEDDylation pathway activator to inhibit the tumor progression in vitro and in vivo. VII-31 induces apoptosis via intrinsic and extrinsic pathways.

Storage

Store at -80°C

Verschickt in

Dry ice packs + Cold packs

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Namen und Kennungen

• Isomere SMILES: COC1=CC=C(C=C1)CN(C2=CC(=C(C(=C2)OC)OC)OC)C(=O)CC3=CC=CS3
• PubChem CID: 146673076
• Molekulargewicht: 427.51

Häufig gestellte Fragen

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2305757-96-2, the molecular formula is C23H25NO5S, and the molecular weight is 427.51 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.