Yeast RNA Extraction Kit (Alkaline Method)

Cat. No.: Y1527228

GRADE & PURITY BioReagent

Übersicht

RNA sources are diverse, and various extraction and preparation methods have been established. Commonly used methods include the phenol method (i.e., the Trizol method), detergent‑based methods, and the guanidinium hydrochloride method, among which the Trizol method is the most widely used. Trizol reagent contains phenol, guanidinium thiocyanate, and other components that rapidly lyse cells or tissues and inactivate nucleases (RNases), thereby preserving RNA integrity. In industrial settings, dilute alkali and concentrated salt methods are commonly employed for RNA extraction. Both of these approaches yield denatured nucleic acids and are primarily used for the production of nucleotide raw materials, with relatively simple procedures. This product employs a low‑concentration alkaline solution to disrupt yeast cells, followed by neutralization with acid. After removal of proteins and cell debris, the RNA in the supernatant is collected via ethanol precipitation or by adjusting the pH to 2.5 to precipitate it at its isoelectric point. Yeast cells contain 2.5%–10% RNA but only 0.03%–0.5% DNA, making yeast a commonly used raw material for RNA extraction. This reagent is intended for research use only and is not suitable for clinical diagnosis or other applications.

Specifications

Spezifikationen & Reinheit

BioReagent

Note

BioReagent

Empfindlichkeit

Light Sensitive

Lagerung und Versand

Storage

Room temperature

Verschickt in

Normal

Stabilität und Lagerung

Store at room temperature long term (12 months).

Contents & Storage

Component No.	Component Name	Appearance	50T	Storage
Y1527228A	RNA Alkaline Lysis Solution (20×)	Liquid	100ml	RT
Y1527228B	RNA Acidic Neutralization Solution	Liquid	30ml	RT
Y1527228C	RNA Precipitation Solution (5×)	Liquid	100ml	RT